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41 results about "Well differentiated" patented technology

The words "well-differentiated", "moderately differentiated, and "poorly differentiated" describe tumor grade. "Well differentiated" usually means a lower grade tumor in which the cells look more like normal cells. "Poorly differentiated" usually means a higher grade tumor in which the cells look more abnormal.

Method for the determination of a nucleic acid using a control

The present invention is directed to a method for the determination of a target nucleic acid using a special control nucleic acid, a method for the amplification of a partial sequence of said target nucleic acid using primers, a special control and a kit containing said control. The sequence of these control nucleic acids are at least in part parallel-complementary to the sequence of the target nucleic. These controls have similar properties as the target nucleic acid in hybridization and amplification methods, but can be well differentiated from the target nucleic acid by their different sequence.
Owner:ROCHE MOLECULAR SYST INC

Use of mycobacterium brumae to treat bladder cancer

InactiveUS20150224151A1Reduce riskOrganic active ingredientsBiocideMycobacterium brumaeLymphatic Spread
Use of Mycobacterium brumae for the treatment of bladder cancer. The present invention relates to the treatment of bladder cancer, preferably superficial, non-invasive bladder cancer by using mycobacteria Mycobacterium brumae. Therapy described in this invention is particularly effective in grade 1 (well differentiated) and grade 2 (moderately differentiated) tumor cells. This makes it possible to treat the disease, which is still in an early stage, by reducing the risk of disease progression to a more harmful stage and / or preventing the risk of metastasis.
Owner:AUTONOMOUS UNIVERSITY OF BARCELONA

Methods to enhance rnai oligonucleotide delivery to respiratory epithelial cells

ActiveUS20130281372A1Reducing level of targetReduced mRNA levelOrganic active ingredientsPeptide/protein ingredientsCell biologyWell differentiated
The present invention relates to methods of reducing a level of a target mRNA in a well-differentiated airway epithelial cell by contacting the cell with a sensitizing agent followed by contacting the cell with a therapeutic RNAi agent.
Owner:UNIV OF IOWA RES FOUND

Colon tumor specific binding peptides

Phage display was used to screen peptide libraries that distinguish between well-differentiated (HCT116) and poorly-differentiated colon carcinoma cells (HT29). The screening protocol used selection and subtraction on intact, viable cells, resulting in phage libraries exhibiting high binding selectivity for the poorly-differentiated HT29 cells. A nine amino acid, disulfide-constrained peptide (RPM) was identified that selectively bound and was internalized into colon cancer cells. The peptide may be used to detect colon cancer cells and also may be used to selectively deliver therapeutic agents to the cells.
Owner:UNIV OF UTAH RES FOUND

Horn antenna combining horizontal and vertical ridges

A horn antenna combining horizontal and vertical corrugations. It is made up of two well differentiated parts, the first part being an antenna with horizontal corrugations, i.e. parallel to the axis of propagation, and a second part with vertical corrugations, i.e. transverse to the axis of propagation. The aperture of the arrangement of the corrugations, in the two parts, can preferably follow linear or Gaussian functions.
Owner:UNIV PUBLICA DE NAVARRA PAMPLONA

Rhizosphere simulated culture device and method for culturing rhizosphere by using same

The invention provides a rhizosphere simulated culture device and a method for culturing rhizosphere by using the same, belongs to the technical field of agriculture and aims to solve the problems that by the conventional method, the plants are cultured in one type of culture box, the rhizosphere environment can only be studied as a whole, the microenvironments of different rhizospheres cannot be well differentiated, and the workload is huge, the interference factors are too many, the error is big, the precision is low and the like. A culture bowl (1) of the rhizosphere simulated culture device is provided with seeding holes (2), an organic glass plate (3) is fixed on the bottom surface of the culture bowl (1), the culture bowl (1), on which the organic glass plate (3) is fixed, is put into a groove (5), and the groove (5) is embedded into a base (4). The culture method comprises the following steps of: 1, using the rhizosphere culture device; 2, loading soil; 3, fixing the organic glass plate; 4, sowing; 5, placing the culture bowl into the groove; and 6, culturing. The rhizosphere micro-ecological culture device can be used for detecting the micro-ecological environment of the rhizosphere of plants.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Human well-differentiated liver cancer cell line HL1017 and construction method thereof

The present invention discloses a human well-differentiated liver cancer cell line HL1017, wherein a preservation number is CCTCC NO:C201357, and the cell line has the most important functions of synthesis, metabolism and detoxification of liver. In addition, the present invention further discloses a construction method of the human well-differentiated liver cancer cell line HL1017.
Owner:NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV

Parts with different surface finishes and the procedure to obtain them

Disclosed are pieces that are produced by sintering, which have well-differentiated surface finish areas including rough areas resulting from the sintering process, the roughness being determined by the size of grain used in sintering, and polished areas that have undergone a three-dimensional machining process. Also, dislosed is a method that, after obtaining a file containing a representation of the piece to be produced, comprises: selecting the parts to be raised; producing the piece by means of sintering; and subsequently subjecting the piece to automatic, semi-automatic or directed programming generation so that, by means of machining, the raised parts are reduced. Consequently, the resulting piece is economical in terms of costs and exhibits a very high degree of precision in the machined areas, a tolerance 9, and improved finish, texture and finishing in the sensitive areas of the piece.
Owner:PHIBO CAD CAM

A transmission method of physical broadcasting channel aerial information

The invention discloses a method for transmitting the antenna information of a physical broadcast channel and is applied in a wireless communication system, and according to the transmission antenna number of the physical broadcast channel and which time the current sending belongs to in a period, a sending end selects a corresponding scrambling sequence for implementing the scrambling of original information bits, generates a cyclical redundancy check (CRC) code, and sends out the CRC code together with the data; wherein, two antennas and four antennas are corresponding to different scrambling sequences. The method of the invention can well detect the antenna information of the physical broadcast channel and is characterized in that the two situations with two antennas and four antennas are well differentiated, thus ensuring the decoding performance of other physical channels.
Owner:ZTE CORP

Single-polarization synthetic aperture radar image pseudo colorization method

InactiveCN107392877AFacilitate interpretationStrong visual distinctionImage enhancementImage analysisColor imageChannel data
The present invention discloses a single-polarization synthetic aperture radar image pseudo colorization method. The method includes the following steps that: 1, the amplitude data of a single-view SAR image are obtained, negative exponential transformation is performed on the amplitude data, the transformed amplitude data are normalized to an interval of [0, 1]; 2, the grayscale u of the interval of [0,1] is corresponding to the grayscale U of an interval of [0,255]; 3, the grayscale of the interval of [0,255] is divided into 4 layers, and each layer of pixel grayscale is transformed differently, so that RGB data can be obtained; and 4, the RGB-channel data obtained in the step 3 are synthetized into a pseudo-color image. With the method of the invention adopted, the obtained pseudo-color image has a unique noctilucent effect; an artificial target with strong scattering and a natural region with weak scattering can be well differentiated, in specific, the weak scattering region embodies deep blue, the strong scattering target embodies orange; and since evident visual discrimination can be realized, target judgment and interpretation can be benefitted.
Owner:中国科学院电子学研究所苏州研究院

Method for improving audio classification accuracy through mixed component clustering Fisher scoring algorithm

The invention discloses a method for improving the audio classification accuracy through a mixed component clustering Fisher scoring algorithm. The method includes the steps that all class of GMMs are united, and Gaussian components are combined into one Gaussian; a CGMM is formed; Fisher transform is performed on the CGMM; the Fisher score is solved to obtain the equal length characteristic. According to the method for improving the audio classification accuracy through the mixed component clustering Fisher scoring algorithm, each class of GMMs are united; the Gaussian components are combined into one Gaussian; the CGMM is formed; Fisher transform is performed on the CGMM; and the Fisher score is solved to obtain the equal length characteristic. The method combines the advantages of a generative mode and the advantages of a discriminant model, the differentiating characteristics among classes can be described, details can be well differentiated, and particularly when the fragment length of an extracted characteristic is small, high classification accuracy can still be achieved. Through the method, the classification accuracy of six voices can reach 77 percent.
Owner:DEZHOU UNIV

Esophageal cancer marker

As a result of immunohistochemical staining of human cancer pathological tissue sections and normal tissue sections with various marker candidates, the antibody against the SLC38A4 protein, out of 80 spontaneously developed types of antigen-specific antibodies against a transporter, was found to react specifically to esophageal cancer tissue and in particular to show high reactivity in esophageal cancer tissue in early well-differentiated stages.
Owner:BEIJING B&M BIOTECH CO LTD +1

Preparation method of clinical-grade neural stem cell

The disclosure provides a preparation method of a clinical-grade neural stem cell. The preparation method comprises the following steps of a), providing a single neural stem cell; b), inoculating theneural stem cell into a conventional culture flask, and subsequently replacing with a low-adsorption culture flask; c), adding a tumor necrosis factor-alpha, albumin, B27 and an animal-free protogonocyte factor into a culture medium to stimulate the multiplication of the neutral stem cell; d), carrying out half-amount medium change according to a growth status; e), carrying out digestion and passage according to the growth status; f), harvesting to obtain a high-purity, high-activity and further high-differentiation-potential clinical-grade neural stem cell. The method provided by the disclosure is simple to operate; the obtained cell is high in purity and further high in multiplication capacity, and the neutral stem cell prepared through the method provided by the disclosure meets a clinical-grade requirement.
Owner:JILIN TUO HUA BIOTECH

Method for efficiently producing DC-CIK cells through induction of polyinosinic: polycytidylic acid copolymer

The invention relates to the technical field of immunization, in particular to a polyinosinic: polycytidylic acid copolymer (Poly I: C). The polyinosinic: polycytidylic acid copolymer is a ligand of atype-3 Toll-like receptor in an animal body, can mediate a series of immune reactions of the body after TLR-3 is activated, and has a good promoting effect on specific immunity and non-specific immunity of the body. Compared with a control group, DC-CIK induced by the polyinosinic: polycytidylic acid copolymer has higher proliferation, high differentiation (especially CD3+CD4-CD8+, CTL) and hightumor killing activity. The autologous efficient DC-CIK induced by the polyinosinic: polycytidylic acid copolymer and other immune cells are applied to treatment of clinical tumor patients. The polyinosinic: polycytidylic acid copolymer aims to recover or improve the immune function of the tumor patients, and the immune system of the body is improved to kill and inhibit proliferation of tumor cells. The tumor cell load is reduced, tiny residual lesions are removed, or the proliferation mode of residual tumor / cancer cells is obviously inhibited, factors such as relapse and metastasis are eliminated, the cure possibility is increased, the survival time is prolonged, and the life quality is improved. Therefore, the purpose of treating tumors / cancers is achieved.
Owner:成都源泉生物科技有限公司 +1

Method and an apparatus for determining well or fully differentiated mammal cells

A method and apparatus for the in vitro determination and typification of well-differentiated or fully differentiated mammalian cells is disclosed. The method comprises placing a cell sample on a sampling device, irradiating the cells with infrared light, preparing an FT-IR spectrum from the absorption spectrum, calculating derivatives of the FT-IR spectrum and comparing the derivatives to reference FT-IR spectra for known cell types. The apparatus comprises an infrared source and measuring apparatus capable of recording an absorption spectrum, and software for measurement, evaluation, documentation, and derivation of the absorption spectra. Fourier transformation he method is particularly useful for typing cells from tissue biopsies in preparation for cell transplantation.
Owner:TETEC TISSUE ENG TECH

Molecular markers of well-differentiated early liver cancer and use thereof

The invention provides a group of molecular markers of well-differentiated early liver cancer and a use thereof. The group of molecular markers of well-differentiated early liver cancer comprises phosphoglucomutase-1(PGM1) and sequestra sequestrum-1 (SQSTM1). The invention further provides a use of phosphoglucomutase-1 and sequestra sequestrum-1 as a molecular marker combination of early liver cancer in preparation of liver cancer clinical diagnostic reagents. The invention further provides a liver cancer diagnostic kit, wherein the liver cancer diagnostic kit is characterized by comprising a phosphoglucomutase-1 antibody and a sequestra sequestrum-1 antibody. The invention has the advantage that relatively high detection sensitivity and specificity are provided.
Owner:ZHONGSHAN HOSPITAL FUDAN UNIV

Rotating machine fault diagnosis method based on peak index

The invention provides a rotating machine fault diagnosis method based on a peak index. A peak index is constructed based on the idea of signal separation to overcome the shortcomings of the traditional peak index diagnosis technology and improve the accuracy and reliability of diagnosis. Based on a standard vibration signal, vibration signals acquired in real time are divided into trouble-free vibration signals and mixed signals. A peak index is constructed using mixed signals and trouble-free vibration signals, the peak index is more sensitive to fault diagnosis, and weak fault feature signals can be better detected through signal change. There is less overlap between the value range of peak index under normal operation of rotating mechanical equipment and the value range of peak index under failure of the rotating mechanical equipment, and the peak index changes apparently under different conditions. The peak index is sensitive to the fault of an eccentric shaft, and the fault can be well differentiated from other faults. Rotating machine fault diagnosis is highly precise and reliable.
Owner:GUANGDONG UNIV OF PETROCHEMICAL TECH

Methods of improving rnai in well-differentiated airway epithelia

ActiveUS20150313924A1Organic active ingredientsTissue culturePlatelet-activating factor receptorTarget mrna
The present invention relates to methods of reducing a level of a target mRNA in a well-differentiated airway epithelial cell by contacting the cell with a facilitating agent followed by contacting the cell with a therapeutic RNAi agent, wherein the facilitating agent comprises a Platelet-activating factor receptor (PAFR) ligand.
Owner:UNIV OF IOWA RES FOUND

A Fault Diagnosis Method for Rotating Machinery Based on Peak Index

ActiveCN106017921BSensitive to diagnosisSmall range of valuesMachine gearing/transmission testingMachine bearings testingDiagnosis methodsPeak value
The invention provides a rotating machine fault diagnosis method based on a peak index. A peak index is constructed based on the idea of signal separation to overcome the shortcomings of the traditional peak index diagnosis technology and improve the accuracy and reliability of diagnosis. Based on a standard vibration signal, vibration signals acquired in real time are divided into trouble-free vibration signals and mixed signals. A peak index is constructed using mixed signals and trouble-free vibration signals, the peak index is more sensitive to fault diagnosis, and weak fault feature signals can be better detected through signal change. There is less overlap between the value range of peak index under normal operation of rotating mechanical equipment and the value range of peak index under failure of the rotating mechanical equipment, and the peak index changes apparently under different conditions. The peak index is sensitive to the fault of an eccentric shaft, and the fault can be well differentiated from other faults. Rotating machine fault diagnosis is highly precise and reliable.
Owner:GUANGDONG UNIV OF PETROCHEMICAL TECH

Tumor tissue in-vitro pathological staining kit

The invention discloses a tumor tissue in-vitro pathological staining kit. The tumor tissue in-vitro pathological staining kit is a kit developed aiming at leading Cetuximab to be used in new applications of tumor tissue in-vitro pathological staining, experiments verification finds that after the Cetuximab is subjected to fluorescent labeling and then used for tumor tissue in-vitro pathological staining, normal tissues and tumor tissues can be well distinguished, well-differentiated and poorly-differentiated tumor tissues can be distinguished to certain extent, and the kit can be used for tumor detection, thus realizing new use for approved drugs.
Owner:INST OF AUTOMATION CHINESE ACAD OF SCI

Monoclonal antibody resisting ovarian cancer and application thereof

The invention discloses a monoclonal antibody resisting the ovarian cancer and application thereof. A hybridoma cell strain NM003-1 secreting the monoclonal antibody resisting the human ovarian cancer is preserved in China Center for Type Culture Collection on September 26, 2011 with the preservation number of CCTCC NO: C201174. The hybridoma cell strain NM003-1 secretes the monoclonal antibody NJ003-1 resisting the human ovarian cancer. Expression of a specific antigen of the monoclonal antibody NJ003-1 in ovarian cancer tissue is higher than that in ovarian benign disease tissue; the positive expression rate of the antigen in a poorly differentiated patient is higher than that in a well differentiated patient. The monoclonal antibody NJ003-1 can effectively inhibit clone formation of ovarian carcinoma cells SK-OV-3 in soft agar and can remarkably inhibit growth of human ovarian cancer transplantation tumors in a nude mouse. Obviously, the monoclonal antibody is expected to become medicine for treating the ovarian cancer or to be prepared into a diagnostic reagent for detecting the ovarian cancer.
Owner:潘世扬

Medium composition for in vitro culturing polygoni multiflori radix and method for mass production of seedling using same

The present invention relates to a medium composition for in vitro culturing Polygoni Multiflori Radix and a method for mass production of seedlings using the same. In detail, media that exhibited excellent growth rates upon in vitro culturing Polygoni Multiflori Radix were selected from among 10 different media, and the selected media, when supplemented with the plant growth regulator kinetin incombination with IAA or NAA, were found to allow tuberous roots of seedlings to differentiate well. Further, it was found that culturing in the media under the control of contents of inorganic salts therein led to the growth of plants having well-differentiated tuberous roots and that the plants were well-acclimated to soil.
Owner:KOREA INST OF ORIENTAL MEDICINE
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