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Medium composition for in vitro culturing polygoni multiflori radix and method for mass production of seedling using same

A technology for in vitro culture and mass production, applied in cell culture medium, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of undisclosed medium composition, achieve good differentiation, good soil acclimatization, Excellent growth rate effect

Pending Publication Date: 2018-11-23
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In addition, as an example of technologies related to cultivating or cultivating Polygonum multiflorum, Korean Authorized Patent No. 1272092 discloses a cultivation method of Polygonum multiflorum, Journal of the Korean Forestry Society (J.Korean For.Sor.(2011) Vol.100.No. 2, pp172~177) discloses the in vitro propagation of wild Polygonum multiflorum by cultivating axillary buds. Korean Laid-Open Patent No. 2010-0114741 discloses the cultivation method of Polygonum multiflorum and the Polygonum multiflorum cultivated by this method, but does not disclose information about the present invention. Medium composition for in vitro culture of Radix Polygoni Multiflori and method for mass production of Radix Polygonum multiflorum using same

Method used

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  • Medium composition for in vitro culturing polygoni multiflori radix and method for mass production of seedling using same
  • Medium composition for in vitro culturing polygoni multiflori radix and method for mass production of seedling using same
  • Medium composition for in vitro culturing polygoni multiflori radix and method for mass production of seedling using same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1. Induction of Polygonum multiflorum isolated aseptic plant body

[0040] In order to induce isolated sterile plants of Radix Polygoni Multiflori, after removing the outer skin of the seeds, immerse them in 70% (v / v) ethanol for 30 seconds, then wash them with sterilized water, and soak them in 3% (v / v) ethanol. Surface sterilization was carried out twice in NaOCl (sodium hypochlorite, sodium hypochlorite) solution, 5 minutes each time, for a total of 10 minutes. Seeds that were thoroughly washed with sterilized water 5 or more times and dried on filter paper were inoculated into 20 ml of MS (Murashige & Skoog) solid medium. The basal medium uses the MS medium added with 3% (w / v) sucrose and 0.45% (w / v) plant agar (phyto agar) (MB Cell Company, U.S.), and the pH is adjusted to 5.6 Autoclave at 121°C for 15 minutes after ~5.8 and use. The cultivation is carried out in an incubator with an internal temperature of 25±1°C and a luminosity of 5,000 lux (lux) fo...

Embodiment 2

[0042] Embodiment 2. the production of the Radix Polygoni Multiflori in vitro culture body according to the composition of culture medium

[0043] In order to know the suitable medium composition for the production of Polygonum multiflorum in vitro culture, the in vitro culture of Polygonum multiflorum was carried out under various medium conditions. The media used in the present invention were all purchased from Duchefa, and were added with 3% (w / v) sucrose and 0.45% (w / v) phyto agar.

[0044] In Example 2, in order to culture Radix Polygoni Multiflori in vitro, 10 types of media (medium 1 to 10) were used for culturing. The media used in the present invention were all purchased from Duchefa, and were added with 3% (w / v) sucrose and 0.45% (w / v) phyto agar.

[0045] The medium 1 used in the present embodiment 2 is made of 1650mg / l NH 4 NO 3 , 1900mg / l of KNO 3 , 440mg / l of CaCl 2 2H 2 O, 370mg / l of MgSO 4 ·7H 2 O, 170mg / l of KH 2 PO 4 , 0.83mg / l KI, 6.2mg / l H 3 BO ...

Embodiment 3

[0059] Example 3. Confirmation of Polygonum multiflorum differentiation ability according to the type and concentration of plant growth regulator

[0060] In addition to growth rate for optimal cultivation, the type and concentration of plant growth regulators used for tuber differentiation were optimized.

[0061] Among the plant growth regulators, as a cytokinin (Cytokinin) plant hormone that effectively enhances the differentiation ability of Polygonum multiflorum, it has been confirmed that 1.0ppm of kinetine is effective for root differentiation ( image 3 ). The cytokinins are biosynthesized at the terminal ends of the roots and promote cell division of the plant meristems and are therefore utilized together with auxins in tissue culture. The cytokinins function to break dormancy and inhibit senescence of plant tissues. In addition, it promotes the growth of leaves and lateral buds, and prevents aging of leaves and fruits.

[0062] In addition, the roots of Polygonum ...

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Abstract

The present invention relates to a medium composition for in vitro culturing Polygoni Multiflori Radix and a method for mass production of seedlings using the same. In detail, media that exhibited excellent growth rates upon in vitro culturing Polygoni Multiflori Radix were selected from among 10 different media, and the selected media, when supplemented with the plant growth regulator kinetin incombination with IAA or NAA, were found to allow tuberous roots of seedlings to differentiate well. Further, it was found that culturing in the media under the control of contents of inorganic salts therein led to the growth of plants having well-differentiated tuberous roots and that the plants were well-acclimated to soil.

Description

technical field [0001] The invention relates to a culture medium composition for cultivating Radix Polygoni Multiflori in vitro and a method for mass producing seedlings using it. Background technique [0002] Polygoni Multiflori Radix (Polygoni Multiflori Radix) refers to the root tuber of Polygonum multiflori Radix, a plant belonging to the family Polygonaceae. Polygonum multiflorum is a perennial herb, and is a vine, the root is spindle-shaped or tuberous, with a length of 5-15 cm and a diameter of 3-12 cm. The stem is vine and has joints, the lower part of the stem is woody and hollow, the leaves are staggered and single, and the petiole is long and ovate or heart-shaped. The margins of the leaves are flat and hairless on both sides. The flowers are large conical inflorescences at the end of the stem or on the veins, and greenish-white flowers bloom in October with 5 petals. [0003] The root tuber of Polygonum multiflorum before cutting is 6-12cm in length, 3-12cm in...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G22/25C12N5/00
CPCA01H4/00C12N5/00A01G22/00A01H4/001C12N5/0025C12N2501/30C12N2500/05C12N2500/30
Inventor 姜泳珉文炳哲李佳衍崔芝银
Owner KOREA INST OF ORIENTAL MEDICINE
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