42 results about "Taxus wallichiana var. mairei" patented technology

The invention provides a method for breeding and cultivating Taxus chinensis var mairei, and aims at improving the survival rate of Taxus chinensis var mairei bred through asexual cuttage. According to the method, Taxus chinensis seedlings are asexually bred through cuttage, and then large seedlings of Taxus chinensis and sapling grove are obtained through transplanting and planting to enlarge the cultivated area of Taxus chinensis var mairei. By adopting the specific cultivation management measures of Taxus chinensis var mairei, the technology system for breeding and transplanting Taxus chinensis var mairei is established, and an approach for the artificial reproduction of Taxus chinensis var mairei is hewed out, so that large-scale industrial production of Taxus chinensis var mairei becomes possible, thereby satisfying the market demand for Taxus chinensis var mairei, and providing a good test system for the protection of endangered wild plant resources.

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

The present invention relates to a yew branch and leaf extract, an extraction method and applications thereof. The yew branch and leaf extract is extracted by adopting branches and leaves of Taxus chinensis var. mairei as raw materials, and comprises cephalomannine and 7-epitaxol, wherein the active ingredients provide an inhabitation effect for squamous cells in skin cancers, and especially provide significant treatment effects for anal squamous cell carcinoma, esophagus squamous carcinoma and tonsil squamous cell carcinoma. According to the present invention, resource advantages of the yew are utilized to deep develop, utilize and research the Taxus chinensis var. mairei; and a yew anti-skin cancer component extraction method with a characteristic of simple operation is provided, and an effect of the component in anti-skin cancer application is determined.

The invention discloses a method for induction and subculture of taxus chinensis leaf callus. The method comprises the following steps of: disinfecting tender leaves (which are taken as an explants) of south taxus chinensis growing in the current year, inoculating to a specific induced culture medium, selecting loosen and flaxen callus after culturing for 20 days in dark at the temperature of 20-22 DEG C, transferring into a specific subculture medium, and subculturing 20 days at the temperature of 20-22 DEG C, thus obtaining the taxus chinensis leaf callus. The method for the induction and subculture of the taxus chinensis leaf callus provided by the invention has the characteristics of high inductivity, short callus time, rapid growth rate, low pollution rate and the like; and the method achieves the purpose that the taxus chinensis callus is cultured on large scale through a plant cell culture technology.

The invention discloses a taxus mairei planting method, belongs to the technical field of agriculture, and more specifically, relates to a taxus mairei planting method. The taxus mairei planting method with high survival rate and strong sapling vitality comprises the steps of 1, seed treatment: seeds are soaked in water, are rubbed with hands and are trod by feet; after rubbing, the seeds are washed clean and no impurity is left; 2, seed storage: the seeds of which seed coats are removed are immediately subjected to sand storage treatment, sand which is screened by a sieve is surrounded by bricks to form a square, the height is 20-40 cm, one layer of seeds is covered by one layer of fine sand, the bottom sand is 20 cm high, and the last layer of seeds is covered by one layer of sand which is 20 cm high; thatch is paved on the sand, and standing treatment lasts for 18-24 months; 3, seeding: after 18-24 month sand storage, whether seed embryos are revealed is detected, if so, the culture of seedlings can be performed in the spring or early spring: 4, seedling management; 5, water and fertilizer management.

The invention relates to a nucleotide sequence, a molecular probe and a method for identifying a taxus media seedling. The nucleotide sequence for identifying the taxus media seedling is represented as SEQ ID NO.1; upstream MHSF of the nucleotide molecular probe for identifying the taxus media seedling is represented as SEQ ID NO.2, and downstream MHSR is represented as SEQ ID NO.3; and by means of the nucleotide molecular probe MHSF / MHSR, taxus media is identified with a conventional PCR (polymerase chain reaction) method. The sample consumption is small, and the overall operation can be completed only by a small quantity of samples; accuracy and sensitivity are high; MHSF / MHSR is a specific molecular probe for the taxus media, and negative reaction is caused if T. chinensis or T. cuspidata is detected; and the method is simple, the PCR technology is adopted for detection, time is short, and the detection can be finished by half day.

The invention relates to a taxus chinensis cutting orchard construction method. According to the method, a nursery land is selected at a moist dryland or farmland with deep and loose soil, high organic matter amount, good drainage, and no serious pest and disease; the land is prepared and fertilized, and is ditched for preparing beds; biennial seedlings bred from taxus chinensis seeds from high-quality provenance are selected, wherein robustly and vigorously growing seedlings with full buds an with an average seedling height of 50-55cm and an average base diameter of 0.8-1cm are selected for field planting; and managements such as shading, weeding and fertilizing are carried out in the cutting orchard. With the construction method provided by the invention, cutting orchard construction period is short, operation is simple, and cost is relatively low. A taxus chinensis large-area propagation and cultivation technical problem is well solved. With the method, taxus chinensis fine breed propagation level can be improved.

The invention relates to a taxus chinensis var. mairei cutting seedling-raising method comprising the following steps: a taxus chinensis var. mairei cutting seedbed is constructed; automatic spraying equipment and a sunshade net are mounted; annual or biennial branches newly germinated from the base are selected, and are cut into small sections with lengths of 12-15cm; 2-3 of pinnate compound leaves are left on the upper part of each small section; the sections are soaked for 2-3h in an indolebutyric acid solution with a concentration of 90-100ppm, and are subjected to cutting insertion; after cutting insertion, the humidity should be maintained at 70-80%, and the temperature is set at 25-28 DEG C; 30-40 days after rooting, the sections are provided with a solution with 0.15-0.25% urea and 0.2-0.3% potassium dihydrogen phosphate. The method is simple, and has high operability and low cost. With the method, the taxus chinensis var. mairei rooting rate is high, and cutting seedling-raising effect is significant. The method facilitates development and utilization of taxus chinensis var. mairei.

The invention discloses a Taxus chinensis var. maire embryo in-vitro seedling forming technique, which comprises the following steps: removing aril from Taxus chinensis var. maire seeds, disinfecting by using alcohol and sodium hypochlorite in sequence, removing hard testa, taking embryos out, cleaning the embryos in sterile water, inoculating on a specific test-tube culture medium, performing dark culture, culturing under a lamp into seedlings after the embryos are sprouted, putting test-tube plantlets in an environment at the temperature of 25DEG C, light intensity of 1,500lx, illumination of 12h / d and relative humidity (RH) of about 80 percent for 7 days to complete in-bottle seedling hardening, transplanting the test-tube plantlets to a specific medium, controlling the temperatures atday and night to 20-25DEG C and 15-18DEG C, the light intensity at noon to 25-40klx and the RH to 70-85 percent, completing out-bottle medium seedling hardening after 25 days, and transplanting the test-tube plantlets to the field. By the Taxus chinensis var. maire embryo in-vitro seedling forming technique, the Taxus chinensis var. maire embryos are sprouted to form seedlings in the same year, and a quick method is provided for quickly propagating Taxus chinensis var. maire seedlings and protecting resources.

The invention relates to a taxus chinensis var maire potting substrate and a potting method of Taxus chinensis var maire. The potting substrate comprises, by volume, 15 percent to 25 percent to surface layer mellow soil, 35 percent to 45 percent of grass carbon ash, 15 percent to 25 percent of organic fertilizer and 15 percent to 25 percent of river sand. The potting method of the taxus chinensis var maire comprises the steps that after the potting substrate is stirred evenly, a purple sand pot is selected, the inner diameter of the pot is 5 cm to 10 cm larger than the root system of the taxus chinensis var maire, a drainage hole is formed in the bottom of the pot, the potting substrate accounting for 1 / 3-1 / 2 of the height of the pot is placed at the bottom of the purple sand pot, then a transplanted taxus chinensis var maire nursery stock is placed in the pot, the planting depth needs to be proper, the rootstock of the taxus chinensis var maire is covered with the potting substrate with the thickness of 1 cm to 1.5 cm, and space of 3 cm to 5 cm is reserved between the potting substrate and the face of the pot. The potting substrate comprises organic matter, soil is loose, the ventilation and moisture holding effect is good, the potting method is adopted, and the taxus chinensis var maire can grow well indoors.

The present invention discloses a composite microbial fertilizer, which comprises Pseudomonas fluorescens and Glomus versiforme, wherein the preservation number of the Pseudomonas fluorescens in the China Center for Type Culture Collection is CCTCC M 2010158, the preservation number of the Glomus versiforme in the Bank of Glomales in China (BGC) is BGC NM03C, and the resource number of the Glomus versiforme in the national Natural Science & Technology Resources is 1511C0001BGCAM0032. The present invention further discloses applications of the composite microbial fertilizer in Taxus chinensis var.mairei growth promotion and taxol accumulation. The composite microbial fertilizer can provide excellent strain resources for future development of the special microbial fertilizer for the Taxus chinensis var.mairei.

The invention discloses a Method for removing dormancy of seeds of Taxus chinensis var. mairei and increasing their survival rate, which comprises the steps: (1) selecting seeds; (2) storing with sand; (3) soaking the seeds; and (4) accelerating germination at a constant temperature. The dormancy of seeds is broken through sand storage, seed soaking, constant-temperature germination accelerating and other methods, the seed germination time is shortened, the seed germination rate and the seedling survival rate are increased, the method is scientific and safe, the time consumption is low, and the method has good market popularization and application value.

The invention provides a novel coding sequence for coding 2-C-Methyl-D-erythritol4-phosphatecytidyltransferase protein (2-C-Methyl-D-erythritol4-phosphatecytidyltransferase, MCT) expressed in taxus chinensis and application for raising plant terpenoid content. The coding sequence of the invention contains clone, function verification and fusion construction body of the gene, and a novel recombination expression carrier with the construction body. The invention also relates to the conversion of plant cell by the expression vector, and the transgenic plant and the progeny thereof generated by the converted cell, including plant seeds and plant tissue. The gene of the invention is expressed in a plant to raising terpenoid content in the obtained transgenic plant.

The invention discloses a tissue culture method of taxus chinensis, belongs to the technical field of plant culture, and provides the tissue culture method of the taxus chinensis to solve the technical problems of long introduction culture period, high cost and high technical difficulty of taxus chinensis. Middle layer cells of new branches of the taxus chinensis are selected, callus culture and rooting culture are performed, so that the tissue culture period of the taxus chinensis is short, and the survival rate of explants is high; a selected culture medium has good water retention propertyand air permeability, fixation of plants is facilitated, the survival rate of the taxus chinensis planted in forests can be significantly increased to 84% or above, the yield of the taxus chinensis can be significantly increased, and the occurrence rate of disease and pest damage and use amount of pesticides can be reduced.

The invention discloses a preparation method for taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) formula granules. The preparation method comprises the following steps of: after water is added into taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) for dipping, heating to 50-70 DEG C, carrying out stirring and extraction for 1.5-5 h, collecting extracting solutions, adding water into residual medicine residues for repeated extraction, and combining the extracting solutions; after the obtained extracting solution is filtered, carrying out vacuum concentration to obtain a clear cream; drying the clear cream to obtain a dry cream; and after the dry cream is smashed and sieved, obtaining a dry cream powder, then, adding auxiliary materials, evenly mixing, and carrying out granulation. While 10-deacetylation Baccatin III and 7-xyloside-10-deacetylation taxol obtained by the preparation method have a high content transfer rate, a cream yield is relatively high, and 1g of the prepared taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) granules isequivalent to 2.5g of taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) decoction pieces. The invention also provides a control characteristic atlas ofthe taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) granules, and a construction method for the control characteristic atlas, and is used for controlling the quality of the taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) granules, specificity is good, and a repetitive rate is high and stable.

The invention discloses a forest genetic breeding method for taxus chinensis var mairei. The method comprises the following steps of pollen collecting, pollination, fertilizer applying, fruit bearing, sand storage, seeding and transplanting. The method solves the problem that pollination and breeding are difficult due to desynchronization of flower phases among taxus chinensis plant seeds, and is easy and convenient to implement, and a large amount of production practice is carried out in a spraying mode. The method has important and profound significance on deep development and wide application of taxus chinensis var mairei plant resources, and the application prospects are wide.

The invention discloses a preparation and application method of a taxus chinensis var mairei fast-growing synergistic organic fertilizer, the fast-growing synergistic organic fertilizer is applied in the taxus chinensis var mairei vegetative growth period, the growth of taxus chinensis var mairei is promoted, and the medicinal component content of taxus chinensis var mairei leaves is increased. The organic fertilizer prepared by combining resource recycling of the biogas residues, the straw and the rice straw is balanced in nutrition, can improve soil properties, provides comprehensive nutrient elements for growth of taxus chinensis var mairei, promotes growth of taxus chinensis var mairei, and can effectively increase the medicinal component content of taxus chinensis var mairei. The method effectively solves the problems of high energy consumption, recycling of plant waste residues and environmental protection in the medicinal material planting and treatment process, and has good popularization and application prospects.

The invention discloses a cultivation method of the southern yew, which comprises six steps: preparing a soil substrate; selecting a flower pot with drainage holes at the bottom; selecting a young plant of the southern yew; planting; maintenance in a greenhouse; and maintenance at home. The cultivation method of the southern yew of the present invention has the advantages of simple method, readily available raw materials, and low price. The soil substrate of the present invention is rich in organic matter, has air permeability and water retention effects, and can promote the cultivation of the southern yew as an ornamental potted plant indoors. growth, the method of the present invention has greatly improved the indoor maintenance survival rate and survival cycle of the southern yew.

The invention discloses a method for induction and subculture of taxus chinensis leaf callus. The method comprises the following steps of: disinfecting tender leaves (which are taken as an explants) of south taxus chinensis growing in the current year, inoculating to a specific induced culture medium, selecting loosen and flaxen callus after culturing for 20 days in dark at the temperature of 20-22 DEG C, transferring into a specific subculture medium, and subculturing 20 days at the temperature of 20-22 DEG C, thus obtaining the taxus chinensis leaf callus. The method for the induction and subculture of the taxus chinensis leaf callus provided by the invention has the characteristics of high inductivity, short callus time, rapid growth rate, low pollution rate and the like; and the method achieves the purpose that the taxus chinensis callus is cultured on large scale through a plant cell culture technology.

The invention provides a wild imitation method for artificially improving the germination rate of natural taxus chinensis var mairei population seeds and promoting seedling renewal, and relates to the technical field of seedling raising. According to the wild imitation method, in the process of artificially promoting natural taxus chinensis var mairei population recovery, taxus chinensis var mairei seeds stored in sand for one year are sown on the ground without herbaceous layers and with dry branches and fallen leaves removed, and earthing is conducted for 2-6cm. It is proved in the embodiment that the germination rate of the seeds can be remarkably increased (the germination rate of a control group is 0%) by sowing and earthing on the ground without the herbaceous layers and with the dry branches and the fallen leaves removed, and the germination rates of the seeds with the soil covering thicknesses of 2cm, 4cm and 6cm can reach 1.57%, 3.57% and 2.28% respectively. The wild imitating method disclosed is extremely important for promoting near-natural germination and updating of seeds of the natural taxus chinensis var mairei population and recovering and expanding of endangered natural populations.

The invention discloses a forest genetic breeding method for taxus chinensis var mairei. The method comprises the following steps of pollen collecting, pollination, fertilizer applying, fruit bearing, sand storage, seeding and transplanting. The method solves the problem that pollination and breeding are difficult due to desynchronization of flower phases among taxus chinensis plant seeds, and is easy and convenient to implement, and a large amount of production practice is carried out in a spraying mode. The method has important and profound significance on deep development and wide application of taxus chinensis var mairei plant resources, and the application prospects are wide.

The invention provides a taxus chinensis bonsai cultivation method. The method includes steps: soil matrix preparation; flowerpot selecting; seedling selection; planting; bonsai placement, management,flowerpot changing and transplanting; prevention and treatment; dwarfing; trimming and daily maintenance. The taxus chinensis bonsai cultivation method has advantages of simplicity, easiness in raw material acquisition and low cost, and a soil matrix is rich in organic, breathable, effective in water retention and capable of promoting indoor growth of taxus chinensis as an ornamental bonsai. Themethod has advantages that the indoor maintenance survival rate of taxus chinensis is greatly increased while a survival period is prolonged; after pot planting and trimming, the taxus chinensis is attractive in shape and high in ornamental value; besides, the taxus chinensis which belongs to evergreen trees can be put in offices and households to achieve a landscaping effect and is capable of purifying air and adsorbing harmful gases such as carbon monoxide and formaldehyde, an anti-cancer efficacy can be achieved, and accordingly human health is protected.

The invention relates to a pregermination method of taxus mairei seeds. The method comprises the following steps: adopting fresh fruits in November of each year; mixing the aired seeds with silver sand evenly according to the volume ratio of 1 to (2-3), and then storing in a storage pit to April 15 to 30th April in the following year; removing impurities from the seeds, putting into a plastic utensil, and soaking in warm water at 30-37 DEG C for 30-40 hours; continuously spraying warm water at 28-30 DEG C on the soaked seeds for 4-5 days; and storing the processed seeds with the silver sand again until a crack appears on a seed-case. The pregermination method disclosed by the invention is simple in steps, relatively strong in operability and relatively low in cost, and is capable of shortening the sleep time of the taxus mairei seeds, significantly promoting the germination rate of the taxus mairei seeds, and promoting the taxus mairei to grow and breed on a large area.

The invention discloses a method for extracting 10-deacetylbaccatin III from Taxus chinensis, and relates to the technical field of natural product extraction. The method comprises the following steps: (1) crushing; liquid nitrogen treatment; ammonia blasting treatment (2) Petroleum ether ultrasonic removal of impurities; (3) Ethanol high-pressure constant temperature extraction; Ethanol ultrasonic extraction; (4) Concentration under reduced pressure, dissolved in acetone, filtration, and concentration to obtain crude extract one; (5) Upper macroporous adsorption resin , followed by washing with alkaline solution and deionized water, and then eluting with ethanol, collecting the eluent, concentrating to obtain crude extract II; (6) primary recrystallization: recrystallization with acetonitrile; (7) secondary recrystallization Crystallization: Acetone / petroleum ether system is used for recrystallization; the present invention has strong operability, rationally sets each step and strictly controls operating parameters, and can obtain 10-deacetylbaccatin III with excellent purity and yield, with a purity reaching More than 99.3%, the yield is more than 0.0242%.

The invention provides a taxus chinensis var mairei coniferous endophytic fungus strain and application thereof, wherein the strain is Pseudodidymocyrtis lobariellae KL27 and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 14, 2021, and the preservation number is CGMCC NO.22459. According to the strain Pseudodidymocyrtis lobariellae KL27, cultured fermentation broth is sprayed on taxus chinensis var mairei nursery stocks, so that biosynthesis and accumulation of paclitaxel in coniferous leaves of taxus chinensis var mairei can be effectively promoted, and the strain Pseudodidymocyrtis lobariellae KL27 has a relatively high application value.

The invention relates to a building method of a taxus chinensis var mairei cutting orchard for producing erect panicle. The method comprises the steps of removing top tips, adjusting crown bodies, bending main trunks, conducting exposure to promote germination, picking scions and conducting management after picking the scions. By means of the building method, the cutting orchard is short in development cycle, easy to operate and low in cost and well solves the technical problem of common taxus chinensis var mairei cutting seedlings that crowns easily deflect due to a position effect; meanwhile, the yield of the scions of the cutting orchard is greatly increased, the quality of the scions is greatly improved, the method has the advantages of being easy to operate, easy to popularize and thelike, meets large-area breeding and cultivation requirements and can increase the good variety breeding level of taxus chinensis var mairei.

The invention discloses a method for regeneration under forest canopy of standing forest of Pinus elliottii Engelm. The method comprises the following steps: firstly, carrying out intermediate fellingon the standing forest of Pinus elliottii Engelm with the standing forest canopy density of 0.7 or above, carrying out forest land cleaning and land preparation, and then, selecting schima superba, taxus mairei and phoebe bournei as regeneration tree species, wherein the schima superba is planted in the upper middle portion of the forest land, the phoebe bournei and the taxus mairei are planted in the middle lower portion of the forest land, the regeneration tree species are cultivated once in October and November in the same year of afforestation, the regeneration tree species are hoed and subjected to stump cultivation once in September and October in the second year of afforestation, and the regeneration tree species are hoed once in the third year of afforestation. According to the method disclosed by the invention, key measures such as regeneration tree species selection, tree species configuration, intermediate felling of the standing forest and cultivation management are provided, the blank of selection and operation management of regeneration under forest canopy of the standing forest of Pinus elliottii Engelm is filled, and technical support is provided for regeneration of the standing forest of Pinus elliottii Engelm; and the method is popular and easy to understand in technology and high in operability, has a relatively large technical achievement transformation potential and is wide in application prospect.