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Tissue culture method of taxus chinensis

A technology of tissue cultivation and yew, which is applied in the field of plant cultivation, can solve the problems that the amount of yew resources cannot meet paclitaxel, the scarcity of paclitaxel drug sources cannot be solved, and the requirements for cultivation substrates are harsh, so as to achieve good water retention and ventilation. The incidence of pests and diseases, the amount of pesticide usage, and the effect of reducing cultivation costs

Inactive Publication Date: 2019-05-17
QINGYANG DUNBO TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Taxus has strict requirements on the growth environment, especially the cultivation substrate, the introduction and cultivation period is long, the cost is high, and the technology is difficult. Therefore, the amount of artificially cultivated taxus resources at this stage is far from meeting the requirements of paclitaxel production enterprises.
According to the verification by the Ministry of Forestry, the stock of yew raw material forests in the country is very small, and the problem of paclitaxel's scarce drug source is still unsolved in the short term

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for tissue culture of Chinese yew, comprising the following steps:

[0014] ①Treatment of the culture bottle: Soak the culture bottle with 15mg / L nano-zinc oxide alcohol solution for 24 hours, then drain the culture bottle, and then irradiate with ultraviolet light for 40 minutes, and place it in a sterile environment for standby;

[0015] ②Callus cultivation: Select the middle layer cells of the new shoots of Taxus chinensis, process them into 2cm tissue slices, put them into a plant incubator with a wooden shell filled with medium, and cultivate them at 15°C for 15 days, and the light intensity is 10001x. 4 hours of light per day, until a 10cm callus is formed; the medium formula is: 15g / L sucrose, 600mg / L rice bran polysaccharide, 20mg / L γ-aminobutyric acid, 0.5mg / L gibberellin, 550mg / L L calcium hypochlorite, 400mg / L calcium hydroxide, 25mg / L osthole, 10mol / L L-glutamine, pH value is 5;

[0016] ③Rooting culture: select the middle 5cm callus, then steriliz...

Embodiment 2

[0018] A method for tissue culture of Chinese yew, comprising the following steps:

[0019] ①Treatment of the culture bottle: Soak the culture bottle with 15mg / L nano-zinc oxide alcohol solution for 24 hours, then drain the culture bottle, and then irradiate with ultraviolet light for 40 minutes, and place it in a sterile environment for standby;

[0020] ②Callus cultivation: Select the middle layer cells of the new branches of Taxus chinensis, process them into 2cm tissue slices, put them into a plant incubator with a wooden shell containing medium, and cultivate them at 18°C ​​for 20 days, with a light intensity of 12001x. 4 hours of light every day, until a 12cm callus is formed; the medium formula is: 20g / L sucrose, 750mg / L rice bran polysaccharide, 25mg / L γ-aminobutyric acid, 1mg / L gibberellin, 750mg / L Calcium hypochlorite, 550mg / L calcium hydroxide, 32mg / L osthole, 12.5mol / L L-glutamine, pH value is 6.5;

[0021] ③Rooting culture: select the middle 6cm callus, then ster...

Embodiment 3

[0023] A method for tissue culture of Chinese yew, comprising the following steps:

[0024] ①Treatment of the culture bottle: Soak the culture bottle with 15mg / L nano-zinc oxide alcohol solution for 24 hours, then drain the culture bottle, and then irradiate with ultraviolet light for 40 minutes, and place it in a sterile environment for standby;

[0025] ②Callus cultivation: Select the middle layer cells of the new shoots of Taxus chinensis, process them into 2cm tissue slices, put them in a plant incubator with a wooden shell filled with medium, and cultivate them at 16°C for 18 days, and the light intensity is 11001x, Illuminated for 4 hours a day, until a callus of 11 cm was formed; the medium formula was: 17g / L sucrose, 650mg / L rice bran polysaccharide, 22mg / L γ-aminobutyric acid, 0.8mg / L gibberellin, 600mg / L L calcium hypochlorite, 450mg / L calcium hydroxide, 28mg / L osthole, 11.5mol / L L-glutamine, pH value is 5.5;

[0026] ③Rooting culture: select the middle 5.5cm callus...

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PUM

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Abstract

The invention discloses a tissue culture method of taxus chinensis, belongs to the technical field of plant culture, and provides the tissue culture method of the taxus chinensis to solve the technical problems of long introduction culture period, high cost and high technical difficulty of taxus chinensis. Middle layer cells of new branches of the taxus chinensis are selected, callus culture and rooting culture are performed, so that the tissue culture period of the taxus chinensis is short, and the survival rate of explants is high; a selected culture medium has good water retention propertyand air permeability, fixation of plants is facilitated, the survival rate of the taxus chinensis planted in forests can be significantly increased to 84% or above, the yield of the taxus chinensis can be significantly increased, and the occurrence rate of disease and pest damage and use amount of pesticides can be reduced.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue cultivation of yew. Background technique [0002] Taxol extracted from the yew plant is a new type of anti-cancer drug developed in recent years, which has a good therapeutic effect on cancer. At the same time, the yew is an endangered and rare species under national first-class protection, especially the wild yew has been degraded. Deforestation is exhausting. Due to the huge demand for anticancer drugs in the market, the artificial cultivation of yew is particularly important. [0003] Currently, yew seedlings are mainly distributed in the origins of yew, such as Fujian, Guangxi, Jiangxi and other places, and their output and quality are easily restricted, and the production cost is relatively high, making it difficult to meet market demand. Therefore, in order to meet the market demand, the yew is now artificially cultivated, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 房世平秦国鹏杨忞蕊
Owner QINGYANG DUNBO TECH DEV CO LTD
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