34 results about "Lipstatin" patented technology

The present invention relates to a novel pharmaceutical composition and process for preparing swallowable rapidity disintegrating methylcellulose and lipstatin derivatives together in combination with a suitable diluent and excipients.

The present invention provides a fermentation process for producing lipstatin comprising the steps of: a) preparing a fermentation medium containing a lipstatin-producing microorganism comprising an oil and an assimilable carbon source, wherein the wt / wt ratio of oil and assimilable carbon source is regulated to achieve an optimal lipstatin biosynthesis by the microorganism; and b) feeding the fermentation medium with an emulsifier, wherein the emulsifier provides an optimal viscosity for the fermentation medium and optimal pH during the fermentation to permit fermentation for lipstatin production. The disclosed process also provides a process for extracting a lipstatin from a fermentation broth.

The invention discloses a method for preparing a weight-reducing medicine orlistat. The method comprises the following steps of using lipstatin as a starting raw material, and under the catalysis of pentacarbonyl iron, making the lipstatin generate reduction reaction with a hydrazine hydrate to obtain the orlistat. The method for preparing the orlistat, which is provided by the invention, is mild in reaction condition and simple to operate; the reaction yield is effectively improved; the hydrazine hydrate is adopted as a reducing agent and a hydrogen source; reaction by-products are few; the after treatment is simple.

The invention provides a method for purifying lipstatin, which comprises the following steps: 1)providing an organic solvent extract of lipstatin; 2)concentrating the organic solvent extract of lipstatin; 3)mixing an concentrate obtained in the step 2) with C5-C20 alkane and a liquid state organic salt, filtering to obtain a filtrate; and 4)cooling the filtrate obtained in the step 3) and crystallizing to obtain the lipstatin. Compared with the prior art, the method has the following advantages and active effect that the simple processes of extraction, concentration and crystallization can realize extraction of lipstatin, the HPLC purity of lipstatin can reach as high as 86% (for instance); and absolute content can reach as high as 74% (for instance). According to the method, defects of high equipment cost, large energy consumption, complex operation and little one-time treatment capacity in prior art can be overcome, production cost is reduced, production efficiency is increased, and the method is suitable for industrial large-production.

The invention belongs to the technical field of medicine and particularly relates to a method for purifying the important intermediate lipstatin of orlistat serving as a weight-reducing aid. The method provided by the invention comprises fermentation liquor pretreatment, extracting, phase inversion and concentration. The product obtained by using the method is high in purity, and the process provided has the advantages of high yield, short period, simplicity and easiness in control, less equipment menstruum investment and low production cost, and is very suitable for industrial production.

The invention discloses a method for producing Lipstatin through multi-stage fermentation, comprising the stages of strain activation, seed culture and fermentation culture. The first 10-40 hours of the fermentation culture stage is a cell growth stage, with dissolved oxygen level controlled at 50-100%. The Lipstatin production stage occurs after 40 hours. When the wet weight of bacteria reaches 10-12 g / L, the bacteria are transferred into a micro-oxygen fermentation stage, with the dissolved oxygen level controlled at 20-30%. When glucose concentration is less than 1 g / L, flow feeding with a substrate I is started, and fermentation is conducted for 60-80 hours with the glucose concentration in a fermentation tank controlled in the range of 1.0-1.5 g / L. 10-20 hours after feeding with the substrate I, flow feeding with a substrate II is begun, and flow feeding with a substrate III is begun 20-30 hours after feeding with the substrate I. The fermentation adopts multi-stage dissolved oxygen control and feeding fermentation in batches, and makes more than 80% of the product secreted outside the mycelium by multi-stage fermentation production of Lipstatin and simultaneously controls the permeability of Streptomyces mycelium. The method has the advantages of low cost, simple operation, short fermentation time, high yield of Lipstatin and easy separation of the product, and is beneficial to industrial production.

In order to overcome the problems of low fermentation unit, high cost of fermentation raw materials, complex and difficult process, and unsuitability for large-scale industrial production in the existing lipastatin fermentation methods, the present invention provides a simple and efficient fermentation method for lipastatin. and fermentation medium, including the following steps: plate separation and slant culture, bacterial strain slant shake flask fermentation screening, shake flask seed liquid preparation, primary seed tank culture, secondary seed tank culture and fermenter culture. In the present invention, the lipistatin fermentation method and fermentation medium obtained by combining optimization strategies such as screening of high-yielding strains by shaking flask fermentation, optimization of medium at all levels, and improvement of fermentation process technology have the advantages of high fermentation unit, low cost of fermentation raw materials, and control of fermentation process. The advantages of simplicity and convenience, no need to add exogenous toxic precursors, etc., are of great significance for improving lipstatin fermentation yield and product quality, and reducing production costs.