A microfluidic device may include a sample distribution network including a plurality of sample chambers configured to be loaded with biological sample for
biological testing of the biological sample while in the sample chambers, the biological sample having a
meniscus that moves within the sample chambers during loading. The sample distribution network may further include a plurality of inlet channels, each
inlet channel being in flow communication with and configured to flow biological sample to a respective
sample chamber, and a plurality of outlet channels, each outlet channel being in flow communication and configured to flow biological sample from a respective
sample chamber. At least some of the sample chambers may include a physical modification configured to control the movement of the
meniscus so as to control bubble formation within the at least some sample chambers. At least some of the sample chambers may include a dried
reagent positioned within the at least some sample chambers
proximate the inlet channels in flow communication with the at least some sample chambers.