The invention discloses collagen and an extraction method thereof. The method includes the steps: 1) refrigerating and
rubbing animal tissues containing the collagen in
liquid nitrogen; 2) extractingthe collagen from the rubbed animal tissues by an acid /
enzyme method to obtain collagen solution; 3) dialyzing and freeze-
drying the collagen solution to obtain the powdery collagen. By the
refrigeration-
rubbing pretreatment method, the animal tissues containing the collagen is refrigerated in the
liquid nitrogen in advance for a period of time and rubbed into
powder under a certain frequency by arubbing instrument, a triple-
helical structure and
thermal denaturation temperature cannot be affected
after treatment by the method, compared with an original acid-extraction method without
refrigeration-
rubbing, the extraction rate of the method is increased by 18%-82%, and the method is of great significance for application of the collagen and collagen-based
biological materials.