The invention provides a purification method of
porcine circovirus II type
virus-like particles. The method is a new purification method developed through experiment measures
centring on the self-characteristics of the PCV2
virus-like particles and the characteristics of an expression
system of the PCV2
virus-like particles. The method comprises the steps that firstly,
cell lysis is achieved through comprehensive utilization of freezing-thawing and ultrasonic treatment measures; secondly, a great amount of
cell debris and
macromolecule impurities are removed by means of a filter plate module, and sterile supernatant liquid is obtained; thirdly, the supernatant liquid is concentrated by means of a 100kD
hollow fiber membrane system, and a concentrated
virus antigen solution is obtained; fourthly, chromatographic purification is conducted by means of a
Sepharose 4FF
molecular sieve chromatographic purification
system in optimized operation conditions. The method is designed closely aiming at the characteristics of the PCV2 virus-like particles, and particularly suitable for the purification of
recombinant baculovirus PCV2 virus-like particles. By means of the method, the impurities can be more efficiently removed and separated to obtain target proteins, and target antigens can be effectively recycled.