The invention provides a liquid storage container which comprises a first integral component and a second integral component, wherein the first integral component that is processed through one-time injection molding comprises a window, a first locking piece and a first sealing piece; the second integral component that is processed through one-time injection molding comprises a container body, a second locking piece and a second sealing piece; the first locking piece and the second locking piece are locked so as to lead the container body and the window to be fixed; and the first sealing piece and the second sealing piece are matched in a compressed manner, so that the container body and the window are sealed up. The invention also provides a manufacturing method of the liquid storage container, which comprises the steps as follows: 1, the window, the first locking piece and the first sealing piece are formed through the first injection molding and are processed through synchronous injection so as to form the first integral component; and 2, the first integral component is positioned in a container body die, and the container body, the second locking piece and the second sealing piece are molded through the second injection molding so as to form the integral component, so that the locking fixation and the sealing between the container body and the window as well as the injection molding of the container body can be achieved at the same time. The liquid storage container achieves firm sealing and short production period.