The invention discloses a cat
coronavirus integrated
nucleic acid detection card box. The cat
coronavirus integrated
nucleic acid detection card box comprises a box body, wherein a
cracking cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the box body from top to bottom, the
cracking cavity communicates with a box cover, and the second cleaning cavity communicates with a reaction cavity arranged at the bottom of the outer side of the box body; plungers with
plunger hole directions perpendicular to a connecting direction are respectively arranged in every two connecting intervals of the
cracking cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity, and hydrophobic liquid sealing materials are arranged in
plunger holes of the plungers; amplification reaction liquid is arranged in the reaction cavity and comprises primer probe mixed liquid of a cat
coronavirus ORF
gene; and a soluble material layer is arranged in the reaction cavity, and
a DNA polymerase solution is embedded in the soluble material layer. The cat coronavirus integrated
nucleic acid detection card box disclosed by the invention can quickly and integrally perform PCR amplification and detection on cat coronavirus under the condition of relatively low cost, and solves the problems of poor
repeatability, time consumption of a detection method, complicated operation and the like in the prior art.