The invention discloses a fertilizer deep application and cultivation method for increasing the no-tillage middle-season rice yield and improving the nitrogen use efficiency. The method comprises thesteps of 1, field pretreatment, wherein an amides systemic herbicide is applied after preceding crops are harvested and before seedling slinging; 2, dry rice-nursery, wherein hybrid rice is selected,seeds are aired on a clear day before sowing, and the seeds are placed in flowing clear water and soaked in the daytime and are fished out, spread and aired in the night; 3, fertilizer deep application, wherein deep application is conducted on a base fertilizer, broadcasting is conducted on an additional fertilizer, the base fertilizer is a nitrogen-phosphorus-potassium mixed fertilizer, deep application can also be conducted after nitrogen, phosphorus and potassium fertilizers are mixed, and the later additional fertilizer is mainly urea; 4, filed seedling slinging, wherein the fertilizer deep application is completed, irrigation field steeping is conducted before seedling slinging; 5, water management, wherein at the seedling stage, a shallow-water layer is kept, at the tillering stage,intermittent irrigation is conducted, and at the yellow ripening stage, air-drying is conducted after the dough stage; 6, filed management. The fertilizer deep application and cultivation method for increasing the no-tillage middle-season rice yield and improving the nitrogen use efficiency is easy to conduct and convenient to operate, effectively reduces ammonia volatilization and nitrogen loss of a no-tillage rice field, improves the nitrogen use efficiency of the no-tillage rice field, and increases the yield of the no-tillage rice field.