A kind of microbial agent jf for the prevention and treatment of corn stalk rot in saline-alkali land and its preparation method

A technology for corn stalk base rot and microbial inoculum, which is applied in the field of microorganisms to achieve the effects of lowering soil pH, convenient transportation and increasing corn stalk thickness

Active Publication Date: 2022-05-06
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few microbial inoculants that can be used for the prevention and control of corn diseases at present, and it is necessary to develop microbial inoculants suitable for the control of corn stalk rot

Method used

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  • A kind of microbial agent jf for the prevention and treatment of corn stalk rot in saline-alkali land and its preparation method
  • A kind of microbial agent jf for the prevention and treatment of corn stalk rot in saline-alkali land and its preparation method
  • A kind of microbial agent jf for the prevention and treatment of corn stalk rot in saline-alkali land and its preparation method

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preparation example Construction

[0030] The present invention provides a kind of microbial inoculant JF and preparation method thereof for preventing and treating corn stalk rot, comprising the following steps:

[0031] Preparation of 1S microbial inoculum powder: seed the single colonies of activated Sonora desert Bacillus B-2, Bacillus paralicheniformis B-26, Sonora desert Bacillus N-16 and Bacillus pumilus N-17 Cultivation and fermentation culture, the four kinds of cultured bacterial liquids are adsorbed by an adsorbent to obtain microbial bacterial agent powder; the four microbial bacterial agent powders are mixed according to the mass ratio of 1:1:1:1 to obtain a mixed powder; wherein, the The four strains mentioned above are all preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC), and the preservation date is March 5, 2020; the preservation number of Sonora desert Bacillus B-2 is CGMCC NO.19454, deputy The preservation number of...

Embodiment

[0041] (1) Compatibility between halophilic and alkaline antagonistic bacteria

[0042] Referring to the determination method of Barbosa, inoculate one functional strain on the solid medium plate of Horikoshi I or modified LB-I or modified LB-II medium, and inoculate another functional strain on the other half vertically. Each treatment was repeated 3 times. Place the plate in a biochemical incubator at 30°C for cultivation, and observe and record the compatibility reaction between the two strains after 24 hours. The two strains that do not produce antibacterial bands are compatible, otherwise they are incompatible. Compatibility results see figure 1 .

[0043] Horikoshi I medium (g / L): glucose 10.0g, peptone 5.0g, yeast extract 5.0g, K 2 HPO 4 1.0g, MgSO 4 0.2g, Na 2 CO 3 10.0g (sterilized separately), 15-20g agar, pH 9.0, sterilized at 121°C for 20min.

[0044] Improved LB-I medium (75.0g / L NaCl): tryptone 10.0g, yeast extract 5.0g, NaCl 75.0g, agar 15-20g, water ...

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Abstract

The invention provides a preparation method of a microbial inoculum JF for preventing and treating corn stalk base rot, which is characterized by comprising the following steps: preparation of 1S microbial inoculum powder: activating activated Bacillus sonorensis B-2, Bacillus paralicheniformis (Bacillus paralicheniformis) B-26, Bacillus sonorensis (Bacillus sonorensis) N-16, Bacillus pumilus (Bacillus pumilus) N-17 single colonies were cultured by seed and fermented, respectively, The cultured four bacterial liquids are adsorbed with an adsorbent to obtain a microbial inoculum powder; the four microbial inoculum powders are mixed according to a mass ratio of 1:1:1:1 to obtain a mixed powder; the preparation of 2S microbial inoculum JF: The microbial inoculum powder prepared in step 1S is mixed with auxiliary materials to prepare the microbial inoculum JF. The microbial inoculum JF prepared by the invention can effectively improve saline-alkali land and prevent corn stalk rot in saline-alkali land.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial agent JF for preventing and treating corn stalk rot in saline-alkali land and a preparation method thereof. Background technique [0002] As an important land resource, saline-alkali land improvement and utilization have always been the focus and hotspot in the field of agricultural research, and it is also the basis for the development of reserve resources of cultivated land and the potential guarantee for increasing grain production in my country. The global saline-alkali land area accounts for 10% of the world's total area. In China, the total area of ​​saline-alkali land that affects cultivated land exceeds 500 million mu. The area of ​​saline-alkali land ranks third among all countries in the world. Among them, the area with agricultural development potential accounts for the total area of ​​China's cultivated land. More than 10%. The pH value of saline-a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
CPCC12N1/20A01N63/00
Inventor 沙月霞周涛张丽霞任剑孙娇方秋香李明洋聂小雁邢敏
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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