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A kind of microbial agent yf beneficial to the growth of corn in saline-alkali land and its application

A microbial agent, the technology of Bacillus licheniformis, applied in the field of microorganisms, to achieve the effects of improving diversity and structure, good control effect, and increasing emergence rate

Active Publication Date: 2021-04-20
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few microbial inoculants that can be used for the prevention and control of corn diseases at present, and it is necessary to develop microbial inoculants suitable for the control of corn stalk rot

Method used

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  • A kind of microbial agent yf beneficial to the growth of corn in saline-alkali land and its application
  • A kind of microbial agent yf beneficial to the growth of corn in saline-alkali land and its application
  • A kind of microbial agent yf beneficial to the growth of corn in saline-alkali land and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The acquisition of four kinds of bacterial strains of embodiment 1

[0037] (1) Isolation of halophilic bacteria

[0038] Isolation of Halophilus bacillus: Collect the soil of the saline-alkali cornfield in Pingluo County, Ningxia Hui Autonomous Region, add 10g of the sample to 100mL of sterile water, shake fully for about 15min to disperse the soil particles, and then dilute the sample with sterile water. The diluted 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 Spread 0.1mL of each concentration of the sample on Horikoshi I, modified LB-I or modified LB-II medium containing different NaCl concentrations, wrap the coated plate with a parafilm, and place it upside down in a 37°C incubator for cultivation After 3-7 days, single colonies with different phenotypic characteristics were picked from the plate, separated by gradient streaking to obtain pure strains, picked single colonies of purified strains, transferred to 40% glycerol, and stored in a -80°C ultra-low temperature...

Embodiment 2

[0060] The preparation of embodiment 2 microbial bacterial agents YF

[0061] ① Compatibility between halophilic-alkali antagonistic bacteria: refer to Barbosa’s determination method, inoculate one functional strain on half of the LB solid medium plate, and inoculate another functional strain on the other half vertically , each treatment was repeated 3 times. Place the plate in a biochemical incubator at 37°C for cultivation, and observe and record the compatibility reaction between the two strains after 24 hours. The two strains that do not produce antibacterial bands are compatible, otherwise they are incompatible. Compatibility results showed that the four strains did not inhibit each other.

[0062] LB liquid medium: 5g of yeast extract, 10g of peptone, 5g of sodium chloride, 1000mL of water, pH7.4-7.6, sterilized at 121°C for 30min. LB solid medium: add 15g agar powder on the basis of liquid medium.

[0063] ② Preparation of microbial agent YF

[0064]After cryoprese...

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Abstract

The invention provides a microbial bacterial agent YF, which contains four bacterial strains; the four bacterial strains are Bacillus sonorensis B-2 and Bacillus paralicheniformis B-2 respectively. 26. Bacillus sonorensis N‑16 and Bacillus pumilus N‑17, the four strains are all preserved in the General Microorganism Center (CGMCC) of China Committee for the Collection of Microbial Cultures. The date is March 5, 2020; among them, the deposit number of Sonora desert Bacillus B-2 is CGMCC NO.19454, and the deposit number of Bacillus paralicheniformis B-26 is CGMCC NO.19449, Sonora Desert The preservation number of Bacillus N-16 is CGMCC NO.19448, and the preservation number of Bacillus pumilus N-17 is CGMCC NO.19450; the microbial bacterial agent provided by the present invention can effectively prevent corn stalk rot in saline-alkali land and improve saline-alkali soil Quality, significantly promote the growth of corn.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of a microbial agent YF in the prevention and treatment of corn stalk rot in saline-alkali land. Background technique [0002] As an important land resource, saline-alkali land improvement and utilization have always been the focus and hotspot in the field of agricultural research, and it is also the basis for the development of reserve resources of cultivated land and the potential guarantee for increasing grain production in my country. The global saline-alkali land area accounts for 10% of the world's total area. In China, the total area of ​​saline-alkali land that affects cultivated land exceeds 500 million mu. The area of ​​saline-alkali land ranks third among all countries in the world. Among them, the area with agricultural development potential accounts for the total area of ​​China's cultivated land. More than 10%. The pH value of saline-alkali l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20C12N1/205A01N63/22A01P3/00A01P1/00A01P21/00C12R2001/07A01N63/20
Inventor 沙月霞周涛张丽霞任剑孙娇方秋香李明洋聂小雁邢敏
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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