The invention relates to a cleaning and disinfecting device for a puncture sampling drainage tube. The device comprises a cleaning branch supporting plate, a locking device and a cleaning device, wherein the middle of the right end of the cleaning branch supporting plate is provided with a circle hole; the bottom of the right end of the cleaning branch supporting plate is provided with a ring-shaped chute; the ring-shaped chute of the cleaning branch supporting plate is located outside of the circle hole of the cleaning branch supporting plate; the top of the left end of the cleaning branch supporting plate is provided with the locking device; the cleaning device is arranged on the right end of the cleaning branch supporting plate; and the locking device comprises a locking elastic column,a locking clamping cover, a clamping branch chain, an anti-slip clamping cover, a limiting rod, a pressing support, a pressing air cylinder, a pressing sleeve, a supporting elastic rod, a supportingspring and a supporting sleeve. According to the device, the problems that, existing in the prior process of scrubbing and disinfecting the drainage tube, puncture needles on the drainage tube are hard to be pulled out, the cleaning effect of holes on the drainage tube is poor, the cleaning brush is not able to be inserted into the drainage tube due to waggle caused by cleaning of the holes on thedrainage tube are solved.