46results about How to "Doubly magnified" patented technology

The invention belongs to the technical fields of immunoassay and biosensing and discloses a preparation method and application of an electrochemical immunosensor based on an HS-beta-CD-Ag-GOD conjugate. The electrochemical immunosensor is used for rapidly detecting a hog cholera virus antigen CSFV. The manufacturing scheme comprises the following steps: modifying a working electrode a bare platinum carbon electrode by using MWCNTs-CD-Fc-Ab1, and sequentially adding bovine serum albumin, hog cholera virus antigen CSFV and an Ab2-HS-beta-CD-Ag-GOD conjugate. The HS-beta-CD-Ag-GOD conjugate can convert glucose into gluconic acid, two protons and two electrons are transferred to oxygen molecules, hydrogen peroxide is generated, and an HS-beta-CD-Ag nanometer material serves as a mimic enzyme, the reduction of the hydrogen peroxide is catalyzed, the dual amplification of an electrochemical signal is realized, the high sensitivity is realized, and the detection limit can be low to 0.33pg / mL.

The invention discloses a self-assembled nucleic-acid aptamer / protein composite nano probe, a preparation method, a kit and application thereof. According to the invention, three DNA probes, one of which is a DNA probe containing a nucleic-acid aptamer sequence and another is marked with horseradish peroxidase, are hybridized to prepare a self-assembled nucleic-acid aptamer / protein composite nanoprobe; and magnetic nano-particles functionalized by a nucleic-acid aptamer are used as magnetic separation solid-phase carriers for specific capture of human acute lymphoblastic leukemia T lymphocytes (CCRF-CEM) in blood, to improve the capture efficiency of target substances. When the magnetic nano-particles are used in combination with the self-assembled nucleic-acid aptamer / protein composite nano probe, the detection of CCRF-CEM has a wide linear range, a low detection limit, and high precision and accuracy, and high-sensitivity, high-specificity and rapid detection of CCRF-CEM in peripheral blood samples can be realized, which has great significance for auxiliary diagnosis, curative effect evaluation and prognosis judgment of leukemia.

The invention relates to a preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and Au@Ag / CuO-GS as markers, and particularly relates to preparation of a sandwich-type electrochemical immunosensor for detecting pancreatic cancer tumor markers by adopting Au@Ag / CuO-GS as a detection antibody marker. By using excellent biocompatibility and high catalytic performance of the Au@Ag / CuO-GS, the prepared sensor has relatively high sensitivity and relatively wide detection range; and the detection limit can reach 1.5fg / mL.

The invention relates to a preparation method and application of an immunosensor based on a ferritin three-dimensional structure, belonging to the field of novel nano materials and the technical fieldof biosensing. The electrochemiluminescence (ECL) technology is adopted, ferritin is taken as a substrate, ECL luminescent molecules N-(4-Aminobutyl)-N-ethylisoluminol molecules are subjected to covalent cross-linking at the outer portion of the ferritin to obtain isoluminol-ferritin, a reaction condition is regulated and controlled, and the nanogold is reduced at the surface of the isoluminol-ferritin in situ to obtain a novel three-dimensional hybrid nano structure isoluminol-ferritin-nanogold. The present invention further provides a preparation method of an immunosensor based on the structure. The method is simple in preparation, low in cost, low in reaction energy consumption and environmental friendly, the sensor is used for detection of procalcitonin, the sensitivity is high, the specificity is high, the reproducibility is good, the detection limit is 3.4 fg / mL, the linear range is 10 fg / mL-50 ng / mL, and the method has an obvious potential application value in the clinical detection of procalcitonin.

The invention belongs to the technical field of biosensors, and relates to a preparation method and application of an electrochemical biosensor based on DNA walker. The method comprises the followingsteps: firstly, mixing a DNA walkerAFB1 aptamer with hairpin DNA1 and hairpin DNA2, dropwise adding a mixture to the surface of a gold electrode, performing reacting, dropwise adding MCH, and performing incubating at normal temperature to obtain an electrochemical biosensor; then, modifying the surface of the biosensor with AFB1, anchoring a mixed solution of a probe 1, short-chain DNA1 and short-chain DNA 2, soaking the biosensor into a methylene blue solution, and constructing a standard curve by measuring the current value of a sensor and the concentration of the AFB1; and finally, measuring the current value of an unknown sample, and substituting the current value into the constructed standard curve so as to achieve the detection of the AFB1 in the unknown sample. According to the invention, dual signal amplification can be carried out by using an extremely small amount of target AFB1 to obtain a larger methylene blue electrochemical signal, and the method has good detection selectivity, high speed and high sensitivity.

The invention discloses a preparation method of hydrogen peroxide sensor which has electrochemiluminescence and photoelectrochemistry double functions and is constructed on the basis of a functional nano material.The prepared sensor is easy to operate, convenient to carry, rapid in inspection, low in cost and capable of being used for rapid and sensitive detection on hydrogen peroxide in the fields of daily production and life and the like.

The invention discloses a preparation method of a biological immunosensor which has an electrochemiluminescence and photoelectrochemistry dual signal developed strategy and is constructed on the basis of a functional nano material.The prepared immunosensor is easy to operate, convenient to carry, rapid in detection, low in cost and capable of being used for rapid and sensitive detection on parathion-methyl in the fields of daily production and life and the like.

The invention discloses a preparation method of a bifunctional enzyme-free hydrogen peroxide sensor, which is constructed on the basis of a functional nano material. The prepared sensor has the advantages of simple operation, portability, rapidness for detection, and low cost, and can be used to rapidly and sensitively detect hydrogen peroxide in daily life and production.

The invention discloses a preparation method of a biological immunity sensor which is based on functional nano-material construction and has dual signal development strategies of electrogenerated chemiluminescence and photoelectrochemistry, and the prepared sensor is easy to operate, convenient to carry, fast in detection, low in cost and capable of being used for fast and sensitive detection of estradiol in the fields of routine production and life and the like.

The invention provides a probe capable of detecting a nuclear factor-kappa B with high sensitivity, good specificity and short consumed time. The probe is a DNA double-stranded probe; the DNA double-stranded probe is formed by reverse complementation of DNA1 and DNA2; and the nucleotide sequence of the DNA1 is as shown in SEQ ID No. 1, and the nucleotide sequence of the DNA2 is as shown in SEQ IDNo. 2. The invention develops a simple, convenient and sensitive nuclear factor kappa B detection method. According to the method, DNA binding protein, exonuclease III (ExoIII) and an isothermal indexamplification technology are combined, and dual amplification of signals is successfully realized by adopting a molecular beacon dependent amplification fluorescence analysis technology. Compared with other methods, the method has higher specificity and lower detection limit, and can be directly used for detecting the nuclear factor kappa B in a cancer cell nuclear extracting solution.

The invention belongs to the technical field of nanometer functional materials, immunization analysis and biosensing and provides a preparation method and use of a Pd / V2O5 / MWCNTs-based intestinal cancer tumor marker immunosensor. Through use of Pd / V2O5 / MWCNTs as a detection antibody marker, a sandwiched electrochemical immunosensor for detecting an intestinal cancer tumor marker is prepared. Through excellent biocompatibility and high catalytic performances of Pd / V2O5 / MWCNTs, the immunosensor has the advantages of high specificity, high sensitivity and low detecting limit.

The invention discloses a preparation method for a difunctional hydrogen peroxide sensor constructed on the basis of a functional nanometer material and with an autoluminescence excitation function. The prepared sensor is easy to operate, convenient to carry, high in detection speed and low in cost, and can be used for rapidly and sensitively detecting hydrogen peroxide in the fields of daily production and living and the like.

The invention discloses a production method of a biology immunosensor having double signal amplification strategies of electrochemiluminescence and photoelectrochemistry, which is built based on a functional nanometer material. The produced immunosensor is simple to operate, convenient to carry, quick in detection, and low in cost, and can be used for quick and acute detection in the fields of daily production, living and the like.

The invention discloses a production method of a dual-function hydrogen peroxide sensor which is not provided with a peripheral light source and is built based on a functional nanometer material. The produced sensor is simple to operate, convenient to carry, quick to detect, and low in cost, and can be used for quick and acute detection of hydrogen peroxide in the fields of daily production, living and the like.

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of ethinyl estradiol in daily production, life and other fields.

The invention discloses a preparation method for an immune-biological sensor constructed on the basis of a functional nanometer material and with an electrochemiluminescence and photoelectrochemical dual signal amplification strategy. The prepared sensor is easy to operate, convenient to carry, high in detection speed and low in cost, and can be used for rapidly and sensitively detecting ethinylestradiol in the fields of daily production and living and the like.

The invention discloses a detection method of staphylococcus aureus in milk through a double signal amplification effect and belongs to the technical field of biosensing. According to the technology,a gold-platinum nano composite material and DNA mimic enzyme are used for synthesizing nano probes which have the good double catalytic effect on H2O2, a modified electrode made of a graphene / gold nanoparticle nano composite material is used so that the electron transfer capacity of the modified electrode can be improved, and accordingly quick high-sensitivity detection for staphylococcus aureus is achieved. Under the optimized experimental conditions, an electrochemical immunosensor has a wide linear detection range for staphylococcus aureus. In addition, the sensor is successfully applied toquantitative detection of staphylococcus aureus in pure milk and yogurt samples. Compared with traditional methods such as a slab colony counting method, the detection method is convenient and fast,easy to operate and high in sensitivity, and quick high-sensitivity detection for staphylococcus aureus is achieved.

The invention discloses a preparation method of a functional nanomaterial based biological immunosensor with dual signal sending strategies of electrochemiluminescence and photoelectrochemistry.The sensor prepared according to the method has the advantages of simplicity in operation, portability, quickness in detection and low in cost and can be used for rapid and sensitive detection of deltamethrin in fields of daily production, daily life and the like.

The invention discloses a preparation method of a dual-function hydrogen peroxide sensor without peripheral light source constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, convenient to carry, fast to detect, and low in cost, and can be used in daily production, life, etc. Rapid and sensitive detection of hydrogen peroxide in the field.

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of estradiol in daily production, life and other fields.

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of deltamethrin in daily production and daily life.

The invention discloses a method for preparing an enzyme-free dual-function hydrogen peroxide sensor based on a molybdenum sulfide composite material. The prepared sensor is simple to operate, convenient to carry, fast to detect, and low in cost, and can be used in daily production, life and other fields. Rapid, sensitive detection of hydrogen peroxide.

The invention discloses a Goos-Haenchen shift amplifier, a preparation method and an amplification method thereof. The shift amplifier comprises an upper medium and a lower medium with different refractive indexes, the upper layer is a light-transmitting glass prism, and the lower layer is a GH displacement generating device; a concave surface is carved at the right end of the upper-layer glass prism, the concave surface faces the radial inclined right upper part of the displacement amplifier, the size of the concave surface does not exceed 50% of that of the whole glass prism, and the concave surface is positioned at the right end of the displacement amplifier. When light enters the glass prism or the glass sheet to be propagated, the thin layer in contact with the GH displacement generation device can generate reflected light; plasma resonance occurs on the surface when light is reflected on the surface of the GH displacement generation device, Goos-Hanchen displacement is greatly increased, and meanwhile, the light passes through the concave surface of the structure again after being reflected, so that the Goos-Hanchen displacement is amplified again. Double amplification of Goos-Hanchen displacement is achieved, displacement observation and measurement are more convenient, the preparation cost is low, portability is high, and flexibility is high.

The invention relates to a composite hollow microsphere material for a biosensor and a preparation method thereof and belongs to the field of biosensor preparation. The preparation method comprises pretreating a cellulose raw material with an alkali solution, immersing the cellulose raw material in an oxidizing salt solution, carrying out hydrolysis to obtain carboxylated cellulose nanospheres, dispersing the carboxylated cellulose nanospheres in water, adding an organic solvent into the solution, carrying out uniform stirring, adding zinc nitrate hexahydrate into the solution, adding an alkali into the solution drop by drop for a reaction to obtain zinc oxide precursor-cellulose composite microspheres, dispersing the zinc oxide precursor-cellulose composite microspheres in an aqueous solution of graphene oxide to obtain zinc oxide precursor / graphene oxide cellulose composite microspheres, carrying out calcination at a high temperature and removing cellulose to obtain the composite hollow microsphere material. The composite hollow microsphere material is used for a biosensor and significantly increases the sensitivity and stability of the sensor.

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of parathion in daily production and daily life.

The invention discloses a preparation method of a self-luminous excitation dual-functional hydrogen peroxide sensor based on functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in daily production, life, etc. Rapid and sensitive detection of hydrogen peroxide in the field.

The invention discloses a preparation method of a four-electrode system dual-function hydrogen peroxide sensor based on functional nanomaterials. The prepared sensor is simple to operate, convenient to carry, fast to detect, and low in cost, and can be used in daily production, life and other fields. Rapid, sensitive detection of hydrogen peroxide.

The invention discloses a preparation method of a biological immunosensor with electrochemiluminescence and photoelectrochemical dual signal development strategies constructed based on functional nanomaterials. Rapid and sensitive detection of ciprofloxacin in daily production, life and other fields.

The invention discloses a preparation method of a dual-functional hydrogen peroxide sensor based on functional nanomaterials combined with two electrochemical methods. The prepared sensor is simple to operate, convenient to carry, fast to detect, and low in cost, and can be used in daily production Rapid and sensitive detection of hydrogen peroxide in fields such as , life and so on.