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Composite hollow microsphere material for biosensor and preparation method thereof

A biosensor, hollow microsphere technology, applied in chemical instruments and methods, analytical materials, material analysis by electromagnetic means, etc., to achieve the effects of improving stability, low preparation cost, and large specific surface area

Inactive Publication Date: 2018-11-06
CHENDU NEW KELI CHEM SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at problems such as selective activity and biological response stability of existing biosensors, the present invention provides a new method for preparing composite hollow microsphere materials for biosensors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Composite Hollow Microspheres for Biosensors

[0033] Prepare as follows:

[0034] a. The cellulose raw material is pretreated with lye, then immersed in an oxidative salt solution, hydrolyzed, and spray-dried to obtain carboxylated cellulose nanospheres; wherein, the cellulose raw material is cotton cellulose; the oxidative salt is periodate Salt.

[0035] b. Disperse the carboxylated cellulose nanospheres prepared in step a into water, add an organic solvent, stir evenly, then add zinc nitrate hexahydrate, then add alkali dropwise to react, keep the pH value at 11, filter to obtain zinc oxide Precursor-cellulose composite microspheres; wherein, the organic solvent is ethanol; the mass ratio of carboxylated cellulose nanospheres to zinc nitrate hexahydrate is 5.5:1.

[0036] c, then disperse the zinc oxide precursor-cellulose composite microspheres prepared in step b in the aqueous solution of graphene oxide to obtain zinc oxide precursor / gra...

Embodiment 2

[0037] Example 2 Preparation of Composite Hollow Microspheres for Biosensors

[0038] Prepare as follows:

[0039] a. The cellulose raw material is pretreated with lye, then immersed in an oxidizing salt solution, hydrolyzed, and spray-dried to obtain carboxylated cellulose nanospheres; wherein, the cellulose raw material is cotton cellulose; the oxidizing salt is chlorinated acid.

[0040] b. Disperse the carboxylated cellulose nanospheres prepared in step a into water, add an organic solvent, stir evenly, then add zinc nitrate hexahydrate, then add alkali dropwise to react, maintain a pH value of 12, filter to obtain zinc oxide The precursor-cellulose composite microspheres; the organic solvent is propylene glycol; the mass ratio of carboxylated cellulose nanospheres to zinc nitrate hexahydrate is 5:1.

[0041] c, then disperse the zinc oxide precursor-cellulose composite microspheres prepared in step b in the aqueous solution of graphene oxide to obtain zinc oxide precurs...

Embodiment 3

[0042] Example 3 Preparation of Composite Hollow Microspheres for Biosensors

[0043] Prepare as follows:

[0044] a. The cellulose raw material is pretreated with lye, then immersed in an oxidative salt solution, hydrolyzed, and spray-dried to obtain carboxylated cellulose nanospheres; wherein, the cellulose raw material is cotton cellulose; the oxidative salt is hypochlorite salt.

[0045] b. Disperse the carboxylated cellulose nanospheres prepared in step a into water, add an organic solvent, stir evenly, then add zinc nitrate hexahydrate, then add alkali dropwise to react, maintain the pH at 11.5, and then filter to obtain oxidized Zinc precursor-cellulose composite microspheres; the organic solvent is propylene glycol methyl ether; the mass ratio of carboxylated cellulose nanospheres to zinc nitrate hexahydrate is 6.5:1.

[0046] c, then disperse the zinc oxide precursor-cellulose composite microspheres prepared in step b in the aqueous solution of graphene oxide to obt...

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Abstract

The invention relates to a composite hollow microsphere material for a biosensor and a preparation method thereof and belongs to the field of biosensor preparation. The preparation method comprises pretreating a cellulose raw material with an alkali solution, immersing the cellulose raw material in an oxidizing salt solution, carrying out hydrolysis to obtain carboxylated cellulose nanospheres, dispersing the carboxylated cellulose nanospheres in water, adding an organic solvent into the solution, carrying out uniform stirring, adding zinc nitrate hexahydrate into the solution, adding an alkali into the solution drop by drop for a reaction to obtain zinc oxide precursor-cellulose composite microspheres, dispersing the zinc oxide precursor-cellulose composite microspheres in an aqueous solution of graphene oxide to obtain zinc oxide precursor / graphene oxide cellulose composite microspheres, carrying out calcination at a high temperature and removing cellulose to obtain the composite hollow microsphere material. The composite hollow microsphere material is used for a biosensor and significantly increases the sensitivity and stability of the sensor.

Description

technical field [0001] The invention relates to a composite hollow microsphere material used for a biosensor and a preparation method thereof, belonging to the field of biosensor preparation. Background technique [0002] The electrochemical immunosensor has the advantages of good selectivity, high sensitivity, low detection limit, suitable for on-line, easy operation, etc. It can realize online electrochemical detection, sample pretreatment is simple or no pretreatment is required, and the detection is not affected by the physical properties of the sample. , the required equipment is relatively simple, with the characteristics of simplicity, speed, and small size, and has been widely used in the detection of tumor markers. [0003] Biosensors can be divided into microbial sensors, enzyme sensors, cell sensors, tissue sensors and immune sensors according to different molecular recognition elements; according to different signal conversion methods, they can be divided into el...

Claims

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Application Information

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IPC IPC(8): G01N27/327C01G9/02C01B32/198
CPCG01N27/327C01B32/198C01G9/02C01P2004/34C01P2004/80
Inventor 陈庆司文彬
Owner CHENDU NEW KELI CHEM SCI CO LTD
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