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Preparation method of parathion-methyl immunosensor based on combination of two electrochemical methods and application

A methyl parathion, immunosensor technology, applied in the fields of electrochemical variables of materials, nanotechnology for materials and surface science, analysis by chemical reactions of materials, etc., can solve the convenience and limitations affecting operation. Wide range of applications and other issues, to achieve the effect of convenient operation, high selective detection, improved detection sensitivity and detection limit

Inactive Publication Date: 2016-07-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, because electrochemiluminescence sensors require external light signal capture devices such as photodiodes, while photoelectrochemical sensors require external light sources to excite photosensitive materials, this affects the convenience of both operations to a certain extent and limits They are more widely used in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 A kind of methyl parathion immunosensor based on the combination of two electrochemical methods

[0037] A method for preparing a methyl parathion immunosensor based on the combination of two electrochemical methods, the specific preparation steps are:

[0038] (1) Cadmium-doped molybdenum disulfide two-dimensional nanomaterial Cd-MoS 2 Solution preparation:

[0039] The cadmium-doped molybdenum disulfide two-dimensional nanomaterial Cd-MoS 2 The solution is Cd-MoS 2 aqueous solution, the Cd-MoS 2 It is a molybdenum disulfide two-dimensional nanomaterial doped with cadmium ions. The two-dimensional nanomaterial is in the form of a paper sheet with a thickness of about 10-20nm. The Cd-MoS 2 The preparation method is: 0.005g cadmium chloride CdCl 2 , 2mL of 0.1mol / L ascorbic acid solution, 0.5mL of sodium molybdate Na 2 MoO 4 solution and 0.01 g sodium sulfide Na 2 S, after mixing and stirring for 15 minutes, put it into the reaction kettle, and react ...

Embodiment 2

[0057] Example 2 A kind of methyl parathion immunosensor based on the combination of two electrochemical methods

[0058] A method for preparing a methyl parathion immunosensor based on the combination of two electrochemical methods, the specific preparation steps are:

[0059] (1) Cd-MoS 2 Solution preparation:

[0060] The preparation steps are the same as that of Cd-MoS in Example 1 2 The preparation method of the solution, the difference is: CdCl 2 The addition amount of ascorbic acid solution is 0.008g, the addition amount of ascorbic acid solution is 4mL, Na 2 MoO 4 The amount of solution added was 1.0 mL and Na 2 The amount of S added was 0.02 g, and the reaction was carried out at 220° C. for 12 hours.

[0061] (2) Preparation of electrochemiluminescent working electrode W1:

[0062] The preparation steps are the same as those of W1 in Example 1, except that the Cd-MoS prepared in (1) in this example is used 2 Modify the electrode; the concentration of lumino...

Embodiment 3

[0070] Example 3 A kind of methyl parathion immunosensor based on the combination of two electrochemical methods

[0071] A method for preparing a methyl parathion immunosensor based on the combination of two electrochemical methods, the specific preparation steps are:

[0072] (1) Cd-MoS 2 Solution preparation:

[0073] The preparation steps are the same as that of Cd-MoS in Example 1 2 The preparation method of the solution, the difference is: CdCl 2 The addition amount of ascorbic acid solution is 0.01g, the addition amount of ascorbic acid solution is 6mL, Na 2 MoO 4 The amount of solution added was 1.5mL and Na 2 The amount of S added was 0.03 g, and the reaction was carried out at 220° C. for 12 hours.

[0074] (2) Preparation of electrochemiluminescent working electrode W1:

[0075] The preparation steps are the same as those of W1 in Example 1, except that the Cd-MoS prepared in (1) in this example is used 2 Modify the electrode; the concentration of luminol ...

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Abstract

The invention discloses a preparation method of a biological immunosensor which has an electrochemiluminescence and photoelectrochemistry dual signal developed strategy and is constructed on the basis of a functional nano material.The prepared immunosensor is easy to operate, convenient to carry, rapid in detection, low in cost and capable of being used for rapid and sensitive detection on parathion-methyl in the fields of daily production and life and the like.

Description

technical field [0001] The invention relates to a preparation method and application of an immunosensor for detecting methyl parathion, and belongs to the technical field of novel nanometer functional materials and electrochemical biosensing analysis. Background technique [0002] Methyl parathion is a broad-spectrum highly toxic insecticide, which has contact killing, stomach poisoning and fumigation effects, and can penetrate into plants. Methyl parathion has a fast action on insects, and can be used to control cotton, apple, citrus, pear, peach and other fruit tree pests and wheat red spiders. In soil, methyl parathion can slightly migrate to the deep soil layer through water leaching. Methyl parathion in the soil can enter the plant body through the absorption of plant roots. After people accidentally eat such plants or plants containing their residues, methyl parathion can enter the human body through the digestive tract, respiratory tract and intact skin and mucous m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N21/76B82Y30/00
CPCB82Y30/00G01N21/76G01N27/48
Inventor 张勇邓保军庞雪辉任祥魏琴
Owner UNIV OF JINAN
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