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Preparation method and application of a photoelectrochemical sensor for estradiol based on self-luminescence excitation

A self-luminous excitation and photoelectrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, analysis by causing chemical reactions of materials, and electrochemical variables of materials, can solve the problems affecting the convenience of operation and restricting wide application, etc. Achieve the effects of convenient operation, high selectivity detection, important scientific significance and application value

Inactive Publication Date: 2017-07-25
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, since electrochemiluminescence sensors require external light signal capture devices such as photodiodes, while photoelectrochemical sensors require external light sources to excite photosensitive materials, this affects the convenience of both operations to a certain extent and limits They are more widely used in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 A photoelectrochemical sensor for estradiol based on self-luminescence excitation

[0038] A method for preparing an estradiol photoelectrochemical sensor based on self-luminescence excitation, the specific preparation steps are:

[0039] (1) Preparation of electrochemiluminescent working electrode W1:

[0040] 1) Use ITO conductive glass as the working electrode, and drop-coat NH on the surface of the electrode 4 NiPO 4 Solution, covering an area of ​​1 cm × 1 cm, dry at room temperature;

[0041] 2) Immerse the working electrode obtained in 1) into the electrolyte solution, and the immersion area is NH 4 NiPO 4 The area covered by the solution was electrochemically deposited on the working electrode using a three-electrode system. After deposition, the working electrode was taken out, washed with ultrapure water, and dried at 4 °C in the dark to prepare the electrochemiluminescent working electrode W1;

[0042] The NH 4 NiPO 4 The solution is an aqueo...

Embodiment 2

[0059] Example 2 A photoelectrochemical sensor for estradiol based on self-luminescence excitation

[0060] A method for preparing an estradiol photoelectrochemical sensor based on self-luminescence excitation, the specific preparation steps are:

[0061] (1) Preparation of electrochemiluminescent working electrode W1:

[0062] The preparation steps are the same as the preparation steps of W1 in Example 1, the difference is: the ammonium salt addition is 0.3 g in the preparation step of the nickel ammonium phosphate micro-nano material, the phosphate addition is 0.20 g, and the nickel dichloride addition is 0.20 g, the stirring temperature and time were 38 ℃ and 12 h, respectively; the concentration of luminol in the electrolyte was 5 mmol / L, and the concentration of sulfuric acid was 0.5 mol / L; when cyclic voltammetry was used for electrochemical deposition, cycle 25 times .

[0063] (2) Preparation of photoelectrochemical working electrode W2:

[0064] 1) Using ITO conduc...

Embodiment 3

[0070] Example 3 A photoelectrochemical sensor for estradiol based on self-luminescence excitation

[0071] A method for preparing an estradiol photoelectrochemical sensor based on self-luminescence excitation, the specific preparation steps are:

[0072] (1) Preparation of electrochemiluminescence working electrode W1:

[0073] The preparation steps are the same as the preparation steps of W1 in Example 1, the difference is: the ammonium salt addition is 0.4 g in the preparation step of the nickel ammonium phosphate micro-nano material, the phosphate addition is 0.25 g, and the nickel dichloride addition is 0.25 g, the stirring temperature and time were 45 ℃ and 14 h, respectively; the concentration of luminol in the electrolyte was 10 mmol / L, and the concentration of sulfuric acid was 1.0 mol / L; when the electrochemical deposition was carried out by cyclic voltammetry, cycle 30 times .

[0074] (2) Preparation of photoelectrochemical working electrode W2:

[0075] 1) Usin...

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PUM

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Abstract

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of estradiol in daily production, life and other fields.

Description

technical field [0001] The invention relates to a preparation method of a sensor for detecting estradiol, and belongs to the technical field of novel nanometer functional materials and electrochemical biosensing analysis. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Estradiol is a synthetic estrogen that is a steroid. At present, the methods for detecting estradiol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N21/76G01N27/30G01N27/327
CPCG01N21/76G01N27/305G01N27/327G01N33/54306G01N33/54386
Inventor 张勇邓保军马洪敏王耀光魏琴
Owner UNIV OF JINAN
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