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Preparation method and application of electrochemiluminescence and photoelectrochemistry-based difunctional ethinylestradiol sensor

A photoelectrochemical and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions, can solve the problems of restricting wide application and affecting the convenience of operation, etc. Achieve high selectivity detection, convenient operation and simple preparation

Inactive Publication Date: 2016-07-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, since electrochemiluminescence sensors require external light signal capture devices such as photodiodes, while photoelectrochemical sensors require external light sources to excite photosensitive materials, this affects the convenience of both operations to a certain extent and limits They are more widely used in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1A dual-functional ethinylestradiol sensor based on electrochemiluminescence and photoelectrochemistry, the specific preparation steps are:

[0037] (1) Preparation of electrochemiluminescent working electrode W1:

[0038] 1) Using ITO conductive glass as the working electrode, drop-coat AuNiPdNRs on the surface of the electrode, covering an area of ​​1cm×1cm, and dry it at room temperature;

[0039] 2) Immerse the working electrode obtained in 1) into the electrolyte, and the immersion area is the area covered by AuNiPdNRs, and use the three-electrode system to perform electrochemical deposition on the working electrode. After deposition, the working electrode is taken out and cleaned with ultrapure water at 4 °C and dry in the dark to prepare the electrochemiluminescent working electrode W1;

[0040] The AuNiPdNRs is an aqueous solution of a rod-shaped nanomaterial with a core-shell structure. The rod-shaped nanomaterial with a core-shell structure is a rod...

Embodiment 2

[0055] Example 2 A dual-functional ethinylestradiol sensor based on electrochemiluminescence and photoelectrochemistry, the specific preparation steps are:

[0056] (1) Preparation of electrochemiluminescent working electrode W1:

[0057] The preparation steps are the same as those of W1 in Example 1, except that the length of the gold nanorods is 30nm, the concentration of luminol in the electrolyte is 5mmol / L, the concentration of sulfuric acid is 0.5mol / L, and the cyclic voltammetry is carried out During electrochemical deposition, cycle 25 times.

[0058] (2) Preparation of photoelectrochemical working electrode W2:

[0059] 1) Using ITO conductive glass as the working electrode, drop-coat 10 μL TiO on the surface of the electrode 2 NSs, dry at room temperature;

[0060] 2) Put the working electrode obtained in 1) into a muffle furnace, perform annealing treatment at 450°C, and cool to room temperature after treatment;

[0061] 3) Apply 10 μL of the ethinyl estradiol...

Embodiment 3

[0065] Example 3 A dual-functional ethinylestradiol sensor based on electrochemiluminescence and photoelectrochemistry, the specific preparation steps are:

[0066] (1) Preparation of electrochemiluminescent working electrode W1:

[0067] The preparation steps are the same as the preparation steps of W1 in Example 1, except that the length of the gold nanorods is 40nm, the concentration of luminol in the electrolyte is 10mmol / L, and the concentration of sulfuric acid is 1.0mol / L, and the cyclic voltammetry is carried out During electrochemical deposition, cycle 30 times.

[0068] (2) Preparation of photoelectrochemical working electrode W2:

[0069] 1) Using ITO conductive glass as the working electrode, drop-coat 12 μL TiO on the surface of the electrode 2 NSs, dry at room temperature;

[0070] 2) Put the working electrode obtained in 1) into a muffle furnace, perform annealing treatment at 450°C, and cool to room temperature after treatment;

[0071] 3) Apply 12 μL of ...

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Abstract

The invention discloses a preparation method for an immune-biological sensor constructed on the basis of a functional nanometer material and with an electrochemiluminescence and photoelectrochemical dual signal amplification strategy. The prepared sensor is easy to operate, convenient to carry, high in detection speed and low in cost, and can be used for rapidly and sensitively detecting ethinylestradiol in the fields of daily production and living and the like.

Description

Technical field [0001] The present invention involves a preparation method for detecting a sensor for detecting the estrogen. The sensor integrates dual functions of electrochemical chemical light and photoelectric chemistry.It belongs to the new type of nano -functional materials and electrified student material sensing technical fields. Background technique [0002] Environmental estrogen refers to the synthesis, release, transportation, combination, metabolic, etc. of normal endocrine substances in the body after entering the body, to activate or suppress the function of the endocrine system, thereby destroying compounds that maintain the stability and regulation of the body and regulate.There are many types of estrogen, including artificial synthetic compounds and natural estrogen, which are widely distributed in nature.Estosterol is a type of steroid synthetic estrogen.At present, the methods of detecting the estradiolin are mainly chromatography and mass spectrometry.Such m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
CPCG01N21/76G01N27/305
Inventor 颜兆庆张勇马洪敏魏琴杜斌
Owner UNIV OF JINAN
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