The invention discloses a method for detecting quality of Bazhen particles, comprising the following steps of: using octadecylsilane chemically bonded silica as a filler; using acetonitrile-0.1% phosphoric acid solution as a mobile phase, wherein a detecting wavelength is 230nm and a flow velocity of the mobile phase is 0.8-1.2ml / min; precisely weighting a paeoniflorin reference substance, and adding 70% ethanol to prepare a paeoniflorin solution; precisely weighting the Bazhen particles, and placing the particles into an erlenmeyer flask with a plug, then precisely adding 50ml 70%ethanol, sealedly plugging the erlenmeyer flask, weighting the erlenmeyer flask, ultrasonically processing for 28-32min, cooling, then weighting, supplementing losing weight by the 70% ethanol, shaking up; taking 10mul each of the a reference substrate solution and a tested object solution, injecting it into a liquid chromatograph, and then detecting. The method of the invention has advantages of rapid and timely completion, and good stabilization and reappearance, can control product quality, can reduce body damage of working personnel, and can reduce unfavorable influence on working environment.