The invention discloses a food packaging box with a positioning ball toy, and belongs to the technical field of food packaging boxes. The food packaging box comprises a box body, wherein the box body is sleeved with a box cover, a lifting handle is connected to the middle of the upper portion of the box cover, the box cover and the box body are connected through a fixing screw, a placing plate is connected to the interior of the box body; the two sides of the interior of the placing plate are each provided with a positioning mechanism, and each positioning mechanism comprises a supporting guide frame, a positioning plate, a cross-shaped through groove, a cross-shaped sliding block and a positioning spring. The supporting guide frames, the positioning plates, elastic hemispheres, the cross-shaped through grooves, the cross-shaped sliding blocks and the positioning springs are arranged, so that under the mutual cooperation of internal structures of the mechanism, the function of clamping and positioning food is achieved, stable placement of the food is effectively guaranteed; and meanwhile, the stability of food placement is further guaranteed through the elastic hemispheres, the structure is simple, and operation is convenient and fast.