The object of the present invention is to provide a vibrating and mixing device. When shaking at room temperature is required, slide the right vertical rail to the left so that the placing seat is located above the anastomotic block, then slide down the placing seat, pull out the right inserting block, and place the Insert the anastomosis slot into the anastomosis block, tighten the fixing nut to fix the placement seat and the anastomosis block, then put the biological reagent test tube into the placement slot of the placement cavity, and then shake and mix well; when heating and shaking is required, turn the left vertical rail Slide right, so that the heating box is above the anastomotic block, slide down the heating box, pull out the left insertion block, insert the anastomotic slot under the heating box into the anastomotic block, tighten the fixing nut, so that the heating box and the anastomotic block are fixed, and then insert the biological The reagent test tube is placed between the two clamping bars in the heating box, and then heated and oscillated to mix evenly, so that the vibration of the two conditions can be realized. When not working, the placing seat and the heating box are located on the left and right sides above the base. Convenience, the whole operation is orderly without mess.