60results about "Chemical library matter" patented technology

A method and apparatus for imaging a sample are provided. An electromagnetic radiation source generates excitation radiation which is sized by excitation optics to a line. The line is directed at a sample resting on a support and excites a plurality of regions on the sample. Collection optics collect response radiation reflected from the sample I and image the reflected radiation. A detector senses the reflected radiation and is positioned to permit discrimination between radiation reflected from a certain focal plane in the sample and certain other planes within the sample.

A bead dispensing system is provided for delivering small amounts of substances onto substrates. The system can include, for example, a movable support structure having an array of spaced-apart projections depending from its lower side. An attraction source, such as a vacuum, magnetic, and / or electrostatic force, is operable at each projection end region to attract and retain one bead. The projection array can be aligned with an array of bead-receiving regions of a substrate, e.g., an array of spaced-apart wells of a micro-plate or card. In one embodiment, a plurality of reagent-carrying beads are picked up, retained at respective projection end regions, and moved to a location over a multi-well plate. The beads are then released in a fashion permitting each bead to land in a respective well. The system of the invention is particularly useful for fabricating arrays of reagents.

A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.

The present invention provides a chemically reactive surface able to covalently react with substances containing a hydroxyl group and / or amine group, comprising a solid surface having an activated dendrimer polyamine covalently bonded to said surface through a silane containing reagent, wherein the dendrimer polyamine can covalently bind the substance comprising a hydroxyl group and / or amino group. The present invention further provides a method for producing chemically reactive surfaces for binding moieties comprising a hydroxyl group and / or amine group, as well as kits comprising the chemically reactive surface of the invention.

Disclosed are systems and methods for manipulating chemical, biological, and / or biochemical samples, optionally supported on substrates and / or within chambers, for example biological samples contained on chips, within biological chambers, etc. In certain embodiments, an apparatus configured to be able to position a chamber or other substrate in one or more modules surrounding the apparatus is disclosed. The apparatus may be configured to be able to move the chamber or substrate in any set of directions, such as radially, vertically, and / or rotationally, with respect to the apparatus. The apparatus may be manually operated and / or automatically controlled. Examples of modules include, but are not limited to, stacking or holding modules, barcode readers, filling modules, sampling modules, incubation modules, sensor modules (e.g., for determining cell density, cell viability, pH, oxygen concentration, nutrient concentration, fluorescence measurements, etc.), assay modules (e.g., for ELISA or other biological assays), data analysis and management modules, control modules, etc. Sensors, control systems, and the like may also be positioned to facilitate operation of the device. Certain embodiments of the invention may be used, for example, to promote or optimize chemical synthesis or cell or biological growth, for instance, for the production of compounds such as drugs or other therapeutics.

Apparatus, system, and method are provided for cutting a linear charged polymer inside a nanopore. A first voltage is applied to create an electric field in a first direction. A second voltage is applied to create an electric field in a second direction, and the first direction is opposite to the second direction. When the electric field in the first direction and the electric field in the second direction are applied to a linear charged polymer inside a nanopore, the linear charged polymer is cut at a location with predetermined accuracy.

A novel approach for fabricating Monolithic Internal micro Pillars (MIPi) made of SU-8 photoresist is described. A microfluidic chip with the internal pillars (a MIPi chip) was used for cell capturing study. The surface of MIPi was coated with specific antibody and then used for capturing cells by affinity binding. An antibody, anti-EGFR, which has high affinity to lung cancer cells, CL1-5, was coated on the micro pillars. The coated MIPi chip specifically captured the cancer cells that were pumped through the MIPi chip. Simulation and experiment was carried out to compare the effect of different geometry of the micro pillars on the cell capturing rate.

Highly functionalized, porous organosilica particles and methods of their synthesis are described that employ high amounts of functional silane such as 3-mercaptopropyl trimethoxysilane. Silane particle diameters are controlled from less than 1 μm to over 100 μm. The particles have a high surface area due to their advantageous internal structures, which consist of large pores, typically up to 10 μm that are linked by small channels of typically about 20 nm diameter. Isothiocyanate modified fluorescent dyes can enter and react with thiol groups inside the pores. The invention also provides clear silica shells of controlled thicknesses to protect fluorescent signals and provide an independent parameter for distinguishing particle types based on light scattering off different sized particles. The particles are stable and useful for many purposes, particularly for optical bar coding in combinatorial synthesis of polymers such as nucleic acid, polypeptide, and other synthesized molecules.

It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.

Methods and kits for the assessment of the suitability of and / or effectiveness of a target therapy for a TNF mediated-related disorder, such as ulcerative colitis, in a subject, which methods and kits evaluate the presence, absence, and / or magnitude of expression of two or more genes up or down regulated in association with anti-TNF responders in inflammatory gastrointestinal disorders, such as ulcerative colitis

The invention relates to immobilization methods, in particular for immobilizing indicator molecules on supports such as sensors and to sensors having those molecules immobilized to their surface. Non-covalent immobilization of macromolecular indicator molecules on those supports via mechanical interlacing with polymers at the surface of a support and via ionic bonding via charged moieties of indicator molecules and ionic groups on the surface of the support are disclosed.

A computer system comprising a database (100) having a plurality of records, is provided. Each record comprises a filed point representation representing field extrema for a conformation of a chemical structure. The database may include records for multiple conformations of the same chemical structure. Each record can have a searchable index of the filed point representation. In one embodiment the index is bit string. An indexing mechanism for generating an index, a searching mechanism for searching the database and a graphical user interface to enable a user to interface with the database (100) are also provided.

A microarray includes a solid substrate having a surface, the surface having a plurality of binding spots and a plurality of reaction moieties bound to the binding spots. A reaction moiety includes a plurality of polyacetylene monomers, the polyacetylene monomers having a first coupling region and a second coupling region, the first coupling region having a first functional group operable to bind to the binding spot and the second coupling region comprising a second functional group operable to bind to an accessory molecule; and an accessory molecule having a binding region and an analyte reaction region, the analyte reaction region operable to selectively bind to the target analyte, and the binding region operable to bind to the second coupling region of the polyacetylene monomer. Upon binding a target analyte with the reaction moiety, a color change from the polyacetylene monomer occurs and the reaction moiety produces fluorescence.

DNA taggants in which the nucleotide sequences are defined according to combinatorial mathematical principles. Methods of defining nucleotide sequences of the combinatorial DNA taggants, and using such taggants for authentication and tracking and tracing an object or process are also disclosed.

The invention provides systems and methods for evaluating synthetic reactions such as polymer synthesis reactions. Evaluation of a synthetic reaction product can be based on a quantitative measure of synthesis such as coupling efficiency or yield, and various parameters determined from a separation record obtained for the synthetic reaction product. The invention further provides methods for evaluating a separation method based on various parameters determined from a separation record. The invention can be used to select an appropriate treatment for a synthetic reaction product such as a separation treatment or treatments related to further processing for the desired product. The methods are particularly useful for automated selection of treatments for synthetic reaction products.

The present invention relates to assay assemblies, components of assay assemblies, methods of determining one or more properties of a sample liquid, and methods of making components of assay assemblies. The present invention allows the properties of very small volumes of sample liquid to be probed, by providing a first surface having at least one sample liquid wettable surface region defined by a sample liquid repelling boundary, and a second surface, opposed to the first surface, having analyte binding agent immobilised thereon.

A carrier pre-encoded with information sufficient to distinguish it from a heterogeneous population of carriers is disclosed on which a compound can be synthesised. The carrier has two attributes integrally associated therewith, which attributes are detectable and / or quantifiable during synthesis of the compound and which define a code identifying the carrier before, during and after said synthesis, with the proviso that one of said attributes is other than shape, or surface deformation(s) of the carrier. The invention also encompasses a plurality of carriers that are pre-encoded as above and a method of synthesising and deconvoluting a combinatorial library using such carriers.

The invention offers the ability to rapidly synthesize multiple chemical compounds, particularly polymers of varying sequences, in parallel on the surfaces of carrier beads. Tinvention involves attaching up-converting phosphors (UCP's) to beads to create up-converting phosphor-loaded beads (UCP-loaded beads) with unique spectral characteristics. Using a dynamic sorting architecture each bead is cataloged based on its spectral characteristics, assigned a compound or polymer to be synthesized, and subjected to multiple rounds of sorting by a flow cytometer, wherein each round sorts the bead to an appropriate bin for a selected chemical reaction, such as the attachment of a monomeric subunit of the polymer sequence.

Embodiments of the disclosure relate to a biosample cartridge that includes storage slots for holding biosample plates. The cartridge has the same form factor as data tape cartridges to allow the cartridge to be handled by the same robotic mechanisms that handle data cartridges in an automated tape library. One aspect of the disclosure concerns a biosample storage cartridge that has a movable door to provide access to inside the cartridge and a plate holder disposed inside the cartridge. The plate holder includes a plurality of slots for receiving biosample plates that are scanned and processed by the automated tape library.

A method of improving signal-to-noise performance of a probe. A probe or set of probes is provided for hybridization with a sample. The probe or set of probes is hybridized with the sample at a first hybridization stringency over a first hybridization time period. Following the hybridization over the first hybridization time period, the probe or set of probes is hybridized with the sample at a second hybridization stringency over a second hybridization time period. A method of improving signal-to-noise performance of probes on an array includes providing a sample containing sequences that provide perfect complementary matches to sequences contained on at least some of said probes; and hybridizing the probes with the sample, while cycling the stringency of hybridization conditions during the hybridization.

The invention relates to the characterization of samples which are located in their many hundreds up to tens or hundreds of thousands on a sample support plate in a regular pattern, a so-called array, by ionization with matrix-assisted laser desorption and mass spectrometric measurement, for example. The invention proposes that the position of the sample pattern, and thus the position of each sample in the measuring instrument, for example a mass spectrometer, should be determined by measuring at least two finely structured internal position recognition patterns, such as fine crosses. The position recognition patterns are preferably applied as the samples are generated, with the same apparatus which also generates the sample pattern. A mass spectrometer in which laser spots with diameters of only four to five micrometers can be generated, which can preferably be positioned with an accuracy of one micrometer or better, is particularly suitable for the characterization.

A biochip is provided that suppresses nonspecific adsorption or bonding of a detection target substance without coating with an adsorption inhibitor and that has excellent detection sensitivity. The constitution is such that it has a macromolecular substance containing a phosphorylcholine group and an active ester group on a substrate surface of a biochip substrate.

The invention relates to a solid support comprising sites intended for the immobilization of oligonucleotide probes or of proteins or of biological cells having ligands, the sites being provided with chemical species bound to the support by a chemical binding function. The chemical species have a diol group connected to the support by an alkyl chain. Application to biochips.

An integrated processing tool is described comprising a full-wafer processing module and a combinatorial processing module. Chemicals for use in the combinatorial processing module are fed from a delivery system including a set of first manifolds. An output of each first manifold is coupled to at least one mixing vessel. An output of each mixing vessel feeds more than one of a set of second manifolds. An output of each set of second manifolds feeds one of multiple site-isolated reactors of the combinatorial processing module.

Methods and kits for diagnosing schizophrenia are disclosed. The diagnostic method comprises analyzing in a biological sample of a subject a level of A-to-I RNA editing of at least one CNS-expressed gene as set forth in Table 1, wherein an amount of the A-to-I RNA editing of the at least one gene below a predetermined level is indicative of schizophrenia in the subject.

A two-pin liquid handling system for crystallizing biomolecules comprises a pair of interacting pins for holding a droplet of liquid therebetween. Each pin includes a tip spaced predetermined distance from the other pin to define a sample acquisition region. The two-pin liquid handling system includes a temperature and humidity control element for controlling the temperature of the sample acquisition region to cause transpiration of water to and from a droplet of biomolecule solution to promote crystallization of the biomolecule.

A continuous temperature-gradient incubation apparatus designed to solve the problem of moving of liquid vapor generated on the high-temperature side toward the low-temperature side to thereby cause condensation.There is provided a multiwell incubation apparatus having a well-housing vessel made of a heat conducting material and, detachably housed therein, liquid-storing wells, the wells being arranged in transverse rows and longitudinal rows, the multiwell incubation apparatus being also provided with a liquid or gas flow channel or bath for supply of a gas saturated with vapor of the liquid, the multiwell incubation apparatus being capable of maintaining incubation temperatures differing between individual transverse rows of wells with a predetermined temperature difference, so as to realize a temperature series which forms a predetermined temperature difference profile along the longitudinal rows, characterized in that the multiwell incubation apparatus includes a heat source for realizing a temperature which is the lowest in the given temperature series, the heat source being disposed outside the well rows and along the transversely lined up wells close to a first side of the housing vessel; another heat source for realizing a temperature which is the highest in the given temperature series, the heat source being disposed outside the well rows and along a side opposite to the first side; and a separator provided for each transverse row of a certain temperature.

"Threedimensional" polymers of very small size can be used in the context of synthesis. These polymers provide simple isolation routes by avoiding the need for workup following the reactions, preworkup characterization, high yield, high capacity and reusability. Of equal importance, these polymers provide a more efficient route to combinatorial synthesis of small molecules. This provides greatly enhanced efficiency for making and screening molecules for physical / biological properties.