The invention belongs to the technical field of
fluorescence analysis, relates to a fluorescent dye containing a
quaternary spiro structure, and a preparation method and application thereof, and concretely relates to a fluorescent dye containing an
azetidine spiro structure, and a preparation method and application thereof. The fluorescent dye containing the
azetidine spiro structure has a skeleton structure as shown in (I) or (II), and the fluorescent dye containing the
azetidine spiro structure has excellent optical properties, good light stability and good
biocompatibility. The invention further provides a novel azetidine spiro-structure-containing fluorescent dye with additional chemical connection sites, a preparation method thereof, and application of the novel azetidine spiro-structure-containing fluorescent dye in biological
fluorescence analysis. The fluorescent molecule shows good water
solubility, light stability and high
fluorescence quantum yield, and can be used for fluorescence labeling,
biological imaging and development of new fluorescent probes.