The invention belongs to the technical field of
plant genetic engineering, and in particular relates to the flowering
gene PtFT2 of trifoliate trifoliate fruit and its application in regulating
plant flowering. The present invention newly discovered a flowering regulatory
gene PtFT2 from Trifoliate Fructus Trifoliate Fructus. Through
gene isolation,
structural analysis,
cloning and
functional verification, the PtFT2 gene was obtained from the
RNA level for the first time. The gene PtFT2 is the spliced version of the PtFT1 gene. Compared with the
genome, the excised version lacks the first
intron in gene structure, and its translation terminates early after the second
exon. The
nucleotide sequence of the gene is shown in SEQ ID NO:1. Transforming tobacco with PtFT2 gene can obviously promote the
flowering time of tobacco earlier, and the gene is expected to play an important role in shortening childhood and earlier flowering of perennial woody fruit trees.