Modified FBXW7 circular RNA and application thereof in tumor drugs and new crown vaccines

A modified and cyclic technology, applied to the modified version of FBXW7 circular RNA and its application in tumor drugs and new crown vaccines, can solve the problem of high productivity, achieve high expression activity, easy preservation, and adaptability strong effect

Active Publication Date: 2021-03-30
楷拓生物科技(苏州)有限公司 +2
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

For example circRNAs undergo rolling circle amplification in an IRES-independent manner, resulting in 100-fold higher productivity than linear transcripts

Method used

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  • Modified FBXW7 circular RNA and application thereof in tumor drugs and new crown vaccines

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Experimental program
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Embodiment

[0011] Customization of linear DNA templates: Firstly, the biological company ordered by DNA synthesis has complementary double-stranded DNA whose template sequences are SEQ ID NO.LD1 and SEQ ID NO.LD2. Both templates include the T7 promoter and the transformation of FBXW7 circular RNA The corresponding DNA sequence of the body RNA, and the corresponding DNA sequence of the RNA of the new coronavirus spike protein and guide protein inserted at the 287-288 position, the RNA sequence is SEQ ID NO.LR3, which is the D614G mutation, and at the 287-288 position The corresponding DNA sequence of p53 protein RNA was inserted respectively, and the RNA sequence was SEQ ID NO.LR4.

[0012] Using linear DNA as the template for amplification, the Invitrogen™ T7 RNA Polymerase Kit (Cat. No.: 18033019) from Thermo Fisher Scientific was used to amplify the RNA. The remaining template was degraded with DNase. The RNA product was purified and then circularized. The RNA Circularization Kit produ...

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PUM

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Abstract

The present invention relates to a circular RNA modified body obtained by modifying a large number of artificial sequences on the basis of a natural FBXW7 circular RNA, the modified body having excellent translation and translation termination effects, and being capable of expressing the protein in vivo after an appropriate protein-encoding RNA sequence is inserted into the insertion site of the modified body. Different proteins can have different effects, so that the modified FBXW7 circular RNA can be used for preparing tumor treatment medicines and new crown vaccines.

Description

technical field [0001] The invention belongs to the field of biotechnology applications, and in particular relates to a modified form of FBXW7 circular RNA, a modified circular RNA modified by an artificial sequence, which has excellent translation and translation termination as a protein-coding RNA sequence Afterwards, the protein can be expressed in vivo, and different proteins can have different effects, which can be used to prepare tumor therapeutic drugs and also to prepare new crown vaccines. Background technique [0002] Vaccines are autoimmune preparations made by artificially attenuating, inactivating or using transgenic methods to prevent infectious diseases from pathogenic microorganisms and their metabolites. Vaccination is an effective way to prevent infectious diseases. Vaccines boost immunity and make an individual resistant to pathogen infection. Vaccines may contain live attenuated pathogens (usually viruses), inactivated whole pathogens, toxoids (inactiva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61K39/00A61K39/215A61P35/00
CPCC12N15/1135C12N15/1131A61K48/00A61K39/001151A61K39/12A61P35/00C12N2310/532A61K2039/53C12N2770/20034Y02A50/30
Inventor 何越峰谭婧文周倩尹锦瑶孙明军毛一竹
Owner 楷拓生物科技(苏州)有限公司
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