30 results about "Stress related genes" patented technology

The invention relates to the technical field of genetic engineering, particularly to a ziziphus jujuba SOD (superoxide dismutase) gene and application thereof. A nucleotide sequence of the ziziphus jujuba SOD gene is indicated in the SEQ ID (sequence identifier) NO (number):1, and an encoded amino acid sequence of the nucleotide sequence is indicated in the SEQ ID:2. The ziziphus jujuba SOD gene and the application thereof have the advantages that the ziziphus jujuba SOD gene is segregated from a ziziphus jujuba tree for the first time, a plant expression vector is constructed, a transgenic arabidopsis plant is obtained, transferring the ziziphus jujuba SOD gene as a target gene into the transgenic arabidopsis plant is practically significant to plant breed improvement, in-depth study of a mechanism of resistance of a high-resistance plant is benefited, molecular structures, expressions, functions and regulations of stress-related genes are defined beneficially, certain experimental data are provided and theoretical bases are established to further improve stress resistance of the plants by means of fully making use of high-resistance genes, understanding of functions of SOD in ROS (reactive oxygen species) signal transaction of the plants is facilitated, and people are helped to understand reactive oxygen serving as signal molecules.

The present invention provides a novel DNA microarray chip that can be used for simultaneous testing of transcriptional responses to cutaneous stressors in the context of neuro-endocrine-immune functions of the skin. The transcriptional responses to ultraviolet radiation in epidermal keratinocytes were tested using such microarray chip containing more than 700 neuro-endocrine-immune related genes. The gene expression pattern was non-random and time dependent; it included increased expression of genes involved in water and salt balance, prostaglandin synthesis, keratinocyte differentiation as well as genes coding for stress effectors, cytokines and metalloproteinases. In contrast, expression was decreased for genes coding for growth factors and their receptors, and for elements of extracellular matrix. This stochastic pattern suggests that transcriptional responses are coordinated and aimed at preservation of epidermal barrier function, prevention of early carcinogenic events and remodeling of extracellular matrix.

The invention provides a wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and application thereof. A pCAMBIA2300-35S-VqbZIP39 overexpressing vector is established for the first time, and is guided into modular Arabidopsis thaliana through a trivia impregnation method, an overexpressing transgenic line of the wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and growth states of a wild contrast under the condition of osmotic stress treatment are researched, physical signs relevant to stress are analyzed, and growth situations of the transgenic line and the wild contrast under conditions of long-term drought stress and salt stress treatment, and expression quantity of a downstream stress related gene are researched. An experiment result shows that by overexpressing of the wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 in the Arabidopsis thaliana, salt stress resistance and drought stress resistance of plants are improved.

The invention provides an application of coded proteins of drought resisting and salt resisting gene of brachypodium distachyon. The nucleotide sequence of the gene is a sequence as shown in SEQ ID NO.1 or a nucleotide sequence complementary as shown in SEQ ID NO.1. The protein is obtained by coding the gene according to the claim 1, and the amino acid sequence of the protein is as shown in SEQ IDNO.2. The gene is applied to improving tolerance of plants to drought and salt resistance. The gene adjusted plant keeps cell moisture by accumulating through matters. ROS accumulation is reduced andthe cytomembrane oxidizing damage caused by active oxygen is reduced by improving the activity of an antioxidant enzyme system. By accelerating closing of pores under drought, the drought and salt resistance of transgenic tobacco is improved by way of reducing water damaged by means of transpiration, improving the expression level of stress related genes and the like. Drought and salt resisncve of the transgenic tobacco with the gene is dependent on adjustment of ABA synthesis and an ABA signal channel.

The invention relates to a rice salt stress related gene SIDP364 and coding protein and application thereof, and relates to a rice gene. The invention provides a rice salt stress related gene SIDP364 and coding protein and application thereof. The rice salt stress related gene SIDP364 can be used for improving the resistance of rice to salt stress and cultivating rice with enhanced high-salt resistance. In a T1 generation overexpressed transgenic plant strain of the rice salt stress related gene SIDP364, the average values of the plant height and the biomass in environment of 200 mmol / L of high salt are higher than those of control obviously, so gene overexpressed transgenic plants can improve the resistance of the rice to the high-salt environment obviously. The gene provides an important path for the cultivation of the rice with the enhanced high-salt resistance. The cultivation of the rice with the high-salt resistance has the great significance on the effective utilization of saline-alkali soil, the increase of grain yield and the like.

The invention belongs to the field of agricultural biotechnology engineering, and particularly relates to an apple rootstock salt stress resistance related gene MdLysMe3 as well as an encoding protein and application thereof. The sequence of the gene is as shown in SEQ ID No.1, and the amino acid sequence of the protein is as shown in SEQ ID No.2. An apple rootstock with enhanced salt stress resistance is obtained by constructing an overexpression vector of the apple rootstock salt stress related gene MdLysMe3 and transforming the overexpression vector to the apple rootstock. The apple rootstock salt stress resistance related gene MdLysMe3 can be used for improving the salt stress resistance of the apple rootstock, and has important significance in expanding the suitable growing area of apple cultivation, accelerating the stress-resistant molecular breeding process and the like.

The invention discloses a corn arsenic stress resistant gene ZmAsR2, and an expression product, cloning primer, linkage SNP and traceable SNP cloning primer thereof. The nucleotide sequence of the corn arsenic stress resistant gene ZmAsR2 is shown as SEQID NO:1, and the amino acid sequence of coded protein is shown as SEQID NO:2. The base sequence characteristic of the intramolecular SNP label ofthe corn arsenic stress resistant gene ZmAsR2 is shown as SEQID NO.5. The intramolecular SNP label of the corn arsenic stress resistant gene ZmAsR2 can be used for tracking and identifying the corn arsenic stress resistant gene ZmAsR2, and is suitable for tracking and identification of corn heavy metal stress tolerance field breeding. The corn arsenic stress resistant gene ZmAsR2 is applied to a corn breeding practical method, and can be promoted and applied to identification cloning, and breeding application of other heavy metal stress related genes.

The invention relates to the technical field of genetic engineering, particularly to a ziziphus jujuba SOD (superoxide dismutase) gene and application thereof. A nucleotide sequence of the ziziphus jujuba SOD gene is indicated in the SEQ ID (sequence identifier) NO (number):1, and an encoded amino acid sequence of the nucleotide sequence is indicated in the SEQ ID:2. The ziziphus jujuba SOD gene and the application thereof have the advantages that the ziziphus jujuba SOD gene is segregated from a ziziphus jujuba tree for the first time, a plant expression vector is constructed, a transgenic arabidopsis plant is obtained, transferring the ziziphus jujuba SOD gene as a target gene into the transgenic arabidopsis plant is practically significant to plant breed improvement, in-depth study of a mechanism of resistance of a high-resistance plant is benefited, molecular structures, expressions, functions and regulations of stress-related genes are defined beneficially, certain experimental data are provided and theoretical bases are established to further improve stress resistance of the plants by means of fully making use of high-resistance genes, understanding of functions of SOD in ROS (reactive oxygen species) signal transaction of the plants is facilitated, and people are helped to understand reactive oxygen serving as signal molecules.

The invention discloses a Europe and America hybrid grape variety Kyoho stress-resistant gene VlbZIP36 and an application thereof. The disclosed gene complete open reading frame sequence is 969 bp, and 322 amino acids are encoded. Transgenic arabidopsis thaliana with overexpression of VlbZIP36 is found out to show enhanced resistance to drought stress in a seed germination stage, a seedling stage and a mature stage, and moreover, the resistance is related with changes of relative conductivity and malonaldehyde and expression enhancement of stress related genes. With use of the VlbZIP36 gene, water loss is restricted, the activity of antioxidase is regulated to remove ROS, the expression level of the related target gene is transcribed and regulated, and the VlbZIP36 gene plays a role in enhancing drought stress resistance in transgenic arabidopsis thaliana. The application disclosed by the invention is an application of the gene VlbZIP36 in improving the ability of plants to resist drought stress.

The invention discloses an ammopiptanthus mongolicus low-temperature stress related gene, and an expression carrier and applications thereof, and belongs to the field of separation and applications of genes relating to plant stress resistance. cDNA sequences of the low-temperature stress related gene separated from ammopiptanthus mongolicus are represented by SEQ ID No.1; and coded amino acid sequences are represented by SEQ ID No.2. The invention also provides the recombined plant expression carrier containing the low-temperature stress related gene, and host cells containing the recombined plant expression carrier. In addition, the invention provides a method used for increasing or improving resistance to plant low temperature stress. The method comprises following steps: the recombined plant expression carrier containing the low-temperature gene is constructed; the recombined plant expression carrier is transformed into receptor plants or plant cells; and gene modified plant varieties are obtained via cultivating and screening. The low-temperature stress related gene separated from ammopiptanthus mongolicus possesses important application prospect in increasing plant resistance to environmental stress or cultivating low-temperature stress resistant plant varieties.

The invention relates to strawberry salt stress related gene FvDIV and application thereof and belongs to the field of gene engineering in molecular biology. A full-length coding region sequence of the strawberry FvDIV for enhancing plant salt stress tolerance is shown as in SEQ ID NO: 1, and its amino acid sequence is shown as in SEQ ID NO: 2. The strawberry salt stress related gene FvDIV provided herein is also used to construct a plant expression vector of FvDIV gene, the constructed plant expression vector transforms Arabidopsis thaliana by means of Agrobacterium-mediated method, and the acquired transgenic plant has significantly improved salt stress tolerance. The strawberry salt stress related gene FvDIV and the application thereof provide theoretical basis and technical means for improving plant salt stress tolerance through gene engineering techniques, and are of great applicable value.

The invention discloses apple MdARF5 gene beneficial for apple callusing, and applications thereof, and belongs to the technical field of molecular biology. The nucleotide sequence of the apple MdARF5 gene is represented by SEQ.ID.NO.1. The invention also discloses an operation method of the apple MdARF5 gene, and applications of the apple MdARF5 gene in callusing of genetically modified apples. According to the applications, beneficial properties, such as plant Al<3+> stress resistance, are improved via plant genetic engineering technology, DNA fragments capable of responding Al<3+> stress-related gene complete coding fragments are obtained via separating and cloning from apples, the functions of the apple MdARF5 gene are verified, and it is found that the sensitivity on Al<3+> stress of genetically modified plants obtained via overexpression of the apple MdARF5 gene in apple callusing and arabidopis thaliana is improved.

The invention relates to a rice salt stress related gene SIDP364 and coding protein and application thereof, and relates to a rice gene. The invention provides a rice salt stress related gene SIDP364 and coding protein and application thereof. The rice salt stress related gene SIDP364 can be used for improving the resistance of rice to salt stress and cultivating rice with enhanced high-salt resistance. In a T1 generation overexpressed transgenic plant strain of the rice salt stress related gene SIDP364, the average values of the plant height and the biomass in environment of 200 mmol / L of high salt are higher than those of control obviously, so gene overexpressed transgenic plants can improve the resistance of the rice to the high-salt environment obviously. The gene provides an important path for the cultivation of the rice with the enhanced high-salt resistance. The cultivation of the rice with the high-salt resistance has the great significance on the effective utilization of saline-alkali soil, the increase of grain yield and the like.

The invention which relates to a salinity and alkalinity stress-induced aquaporin PIP gene of Leymus chinensis belongs to the technical field of genetic engineering. By utilizing an RACE method to clone in the invention, the full length sequence of a strong salinity and alkalinity resistance salinity and alkalinity stress response gene PIP protein gene of Leymus chinensis is obtained, the expression case of the PIP protein gene is detected through the Northern detection and the genetic transformation detection, and the expression case is used to analyze that whether the expression case is related with the salinity and alkalinity stress resistance. The length of the coding region of the obtained PIP protein gene is 879bp, and two hundred and ninety-two amino acids are coded. Compared with barley, wheat and the like which are Gramineae plants, the homology of Leymus chinensis is high, the DNA sequence difference is about 10%, and the amino acid sequence difference is less than 5%. No gene registration information and relevant sequence information reports of the PIP protein gene of Leymus chinensis are reported. Results of PIP protein gene Northern hybridization and gene chip technology researches of the expression of the salinity and alkalinity stress-induced aquaporin PIP gene of Leymus chinensis stressed by the salinity and the alkalinity show that the expression level of the PIP protein gene stressed by the salinity and the alkalinity is increased over two times, so the PIP protein gene is a salinity and alkalinity stress-related gene.

SaRNA capable of activating myocardial hypertrophy related genes and hypoxia stress related genes is screened, myocardial cells are respectively transfected with the saRNA to activate target gene expression, myocardial hypertrophy stimulation and hypoxia stimulation are respectively given on the basis, it is found that the activated target genes have a good protection effect on cells under stress conditions, and therefore, an effective method is provided for cell protection.

The invention discloses an ultraviolet stress-related gene of highland barley and a cloning method thereof. The gene can be used for detecting whether the highland barley suffers from ultraviolet stress or not. The nucleotide sequence of the ultraviolet stress-related gene of the highland barley provided by the invention is as shown in SEQ ID NO.1. The expression level of the gene is related to whether the highland barley suffers from ultraviolet radiation stress or not, whether the highland barley suffers from ultraviolet radiation stress or not can be judged by detecting the expression level of the gene, theoretical support is provided for the control of subsequent growth conditions of highland barley growth, and the gene has a good market application prospect.

The invention discloses a stress related gene PdHARDY of Populus deltoides*Populus nigra, and the stress related gene PdHARDY plays an important role in the salt resisting process of a plant. The stress related gene is named as PdHARDY and has a base sequence as shown in SEQ ID NO:3 in a sequence table. When the stress related gene PdHARDY is constructed in an expression vector pCAMBIA1304, a promoter is added before the transcription initiation nucleotide of the stress related gene PdHARDY, and a selectable marker GFP (Green Fluorescent Protein) is added for conveniently identifying and screening transgenic plant cells or plants; the expression vector carried with the stress related gene PdHARDY can transform plant hosts through multiple methods and be used for culturing salt-resistant plant species. The stress related gene PdHARDY disclosed by the invention has a wide application prospect in culturing salt-resistant plants.

The invention discloses an application of an OsKEAP1 gene in regulation and control of salt stress resistance of rice. The nucleotide sequence of the OsKEAP1 gene is shown as SEQ ID NO. 1. The salt stress resistance is mainly reflected in the content change of hydrogen peroxide, proline and malondialdehyde in the mutant before and after salt stress and the difference of expression levels of salt stress related genes after salt stress treatment. According to the application, the mutant with down-regulated OsKEAP1 gene expression level is obtained by using a CRISPR / Cas9 gene editing technology,and it is found that after OsKEAP1 mutation, the change of an anti-oxidative stress pathway in rice is caused, and then the change of salt resistance of rice is caused, so that a basis can be providedfor culturing rice varieties with excellent salt stress resistance.

The invention provides an application of coded proteins of drought resisting and salt resisting gene of brachypodium distachyon. The nucleotide sequence of the gene is a sequence as shown in SEQ ID NO.1 or a nucleotide sequence complementary as shown in SEQ ID NO.1. The protein is obtained by coding the gene according to the claim 1, and the amino acid sequence of the protein is as shown in SEQ IDNO.2. The gene is applied to improving tolerance of plants to drought and salt resistance. The gene adjusted plant keeps cell moisture by accumulating through matters. ROS accumulation is reduced andthe cytomembrane oxidizing damage caused by active oxygen is reduced by improving the activity of an antioxidant enzyme system. By accelerating closing of pores under drought, the drought and salt resistance of transgenic tobacco is improved by way of reducing water damaged by means of transpiration, improving the expression level of stress related genes and the like. Drought and salt resisncve of the transgenic tobacco with the gene is dependent on adjustment of ABA synthesis and an ABA signal channel.

Rice salt stress-resistance-related gene ONAC103 and its encoded protein and application relate to the technical fields of molecular biology and genetic engineering. A rice salt stress-resistant related gene ONAC103 (LOC_Os07g48450) is disclosed, and the nucleic acid sequence of the gene is shown in SEQ ID 1. ONAC103 is a gene related to salt stress resistance in rice. The relative expression of ONAC103 was significantly up-regulated under salt stress treatment. The salt tolerance of transgenic rice plants overexpressing ONAC103 was significantly higher than that of the control group. This gene can significantly improve the salt tolerance of rice. Therefore, the gene has important application value for screening salt-resistant rice varieties and improving rice yield. The cultivation of rice with enhanced resistance to salt stress in agricultural production is of great significance to energy and water conservation, utilization of saline-alkali land, and increase of grain production.

The invention discloses an ultraviolet stress-related gene of highland barley and a cloning method thereof. The gene can be used for detecting whether the highland barley suffers from ultraviolet stress or not. The nucleotide sequence of the ultraviolet stress-related gene of the highland barley provided by the invention is as shown in SEQ ID NO.1. The expression level of the gene is related to whether the highland barley suffers from ultraviolet radiation stress or not, whether the highland barley suffers from ultraviolet radiation stress or not can be judged by detecting the expression level of the gene, theoretical support is provided for the control of subsequent growth conditions of highland barley growth, and the gene has a good market application prospect.

The invention discloses a stress related gene PdbZIP of populus deltoids*populus nigra (Populus deltoids*Populus nigra). The gene is very important in a salt-resistant process of a plant. The provided bZIP gene is named PdbZIP and has a base sequence shown in SEQ ID NO:3 in a sequence table. A promoter is added before initial nucleotide is transcribed when the stress related gene PdbZIP disclosed by the invention is built in an expression vector pCAMBIA1304; meanwhile, an optional marker green fluorescent protein (GFP) is added, so as to identify and screen transgenic plant cells or plants; the expression vector with the stress related gene PdbZIP disclosed by the invention can transform a plant host by many methods, and is used for cultivating salt-resistant plant variety. The gene disclosed by the invention has a wide application prospect in cultivation of the salt-resistant plant.

The invention discloses a lung adenocarcinoma patient lifetime prognosis evaluation method, a computer device and a storage medium. A prognosis index is calculated by adopting the following formula: prognosis index = beta1 * TNM stage + beta2 * ERSS, wherein beta 1 and beta 2 are weight coefficients, and ERSS is an endoplasmic reticulum stress score. According to the method, correlation between endoplasmic reticulum stress related genes and prognosis of the lung adenocarcinoma patient is evaluated according to a machine learning method, endoplasmic reticulum stress scores are established, the survival prediction model is established in combination with the endoplasmic reticulum stress scores and TNM staging, and experiments show that the survival prediction model has a good prediction effect on the total lifetime, and the survival prediction model can be used for predicting the total lifetime of the lung adenocarcinoma patient. The problem that the lifetime of a patient cannot be accurately evaluated by independently applying TNM staging is solved.

The invention relates to strawberry salt stress related gene FvDIV and application thereof and belongs to the field of gene engineering in molecular biology. A full-length coding region sequence of the strawberry FvDIV for enhancing plant salt stress tolerance is shown as in SEQ ID NO: 1, and its amino acid sequence is shown as in SEQ ID NO: 2. The strawberry salt stress related gene FvDIV provided herein is also used to construct a plant expression vector of FvDIV gene, the constructed plant expression vector transforms Arabidopsis thaliana by means of Agrobacterium-mediated method, and the acquired transgenic plant has significantly improved salt stress tolerance. The strawberry salt stress related gene FvDIV and the application thereof provide theoretical basis and technical means for improving plant salt stress tolerance through gene engineering techniques, and are of great applicable value.

The invention discloses a Europe and America hybrid grape variety Kyoho stress-resistant gene VlbZIP36 and an application thereof. The disclosed gene complete open reading frame sequence is 969 bp, and 322 amino acids are encoded. Transgenic arabidopsis thaliana with overexpression of VlbZIP36 is found out to show enhanced resistance to drought stress in a seed germination stage, a seedling stage and a mature stage, and moreover, the resistance is related with changes of relative conductivity and malonaldehyde and expression enhancement of stress related genes. With use of the VlbZIP36 gene, water loss is restricted, the activity of antioxidase is regulated to remove ROS, the expression level of the related target gene is transcribed and regulated, and the VlbZIP36 gene plays a role in enhancing drought stress resistance in transgenic arabidopsis thaliana. The application disclosed by the invention is an application of the gene VlbZIP36 in improving the ability of plants to resist drought stress.