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A kind of ultraviolet stress-related gene of highland barley and its application

A highland barley and gene technology is applied to the ultraviolet stress-related genes of highland barley and its application field, which can solve the problems of affecting the growth and development of highland barley, no effective means, and yield impact, and achieve the effect of good market application prospects.

Active Publication Date: 2019-07-09
AGRI RES INST TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ultraviolet stress will seriously affect the growth and development of highland barley, and have a greater impact on the final yield
[0004] However, at present, there is no effective means to detect whether the highland barley is exposed to ultraviolet radiation.

Method used

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  • A kind of ultraviolet stress-related gene of highland barley and its application
  • A kind of ultraviolet stress-related gene of highland barley and its application
  • A kind of ultraviolet stress-related gene of highland barley and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Cloning and expression of embodiment 1 gene of the present invention

[0032] 1: Gene cloning

[0033] (1) RNA extraction and reverse transcription

[0034] 1. Use highland barley 320 and hi 8 seeds to germinate and grow into seedlings.

[0035] 2. Take fresh leaves and use plant extraction kit to extract RNA and detect.

[0036] 3. The extraction steps are as follows:

[0037] 3.1 Instruments and reagents

[0038] Main instruments: SCILOGEX D3024R centrifuge, analytikjena-Easycycler; Scientz-48 high-throughput tissue grinder;

[0039] Main reagents: Reverse Transcription Kit (TUREscript 1st Stand cDNA SYNTHESIS Kit) (Aidelai, Cat. No. PC1802), 2× master mix (Germany DBI pcrMix).

[0040] 3.2 Experimental methods and steps

[0041] 3.2.1 Extraction of RNA from tissue samples

[0042] The RNA extraction kit uses Jena innuPREP RNA Mini Kit.

[0043] 3.2.2 cDNA synthesis (reverse transcription)

[0044] Use Aidlab’s reverse transcription kit (TUREscript 1st Stand ...

Embodiment 2

[0116] Embodiment 2 The correlation between the gene of the present invention and the highland barley being subjected to ultraviolet radiation stress

[0117] 1. Materials

[0118] Two seedlings of highland barley have been provided, and the sample information is as follows:

[0119]

[0120] 2. Instruments and reagents

[0121] 2.1 Main Instruments

[0122] analytikjena-qTOWER2.2 fluorescent quantitative PCR instrument (Germany), SCILOGEX D3024R centrifuge (USA), general PCR instrument analytikjena-Easycycler (Germany); ultra-micro nucleic acid protein analyzer scandrop100 (Germany); pipette (USA bio- rad), DNA electrophoresis spectrum viewer (Junyi), electrophoresis instrument (Junyi).

[0123] 2.2 Main reagent consumables

[0124] Reverse transcription kit (TUREscript 1st Stand cDNA SYNTHESIS Kit) (Aidelai (is a brand) product number PC1802,); Green master mix (Germany DBI). 10ul tip (US GCS), 200ul tip (US GCS), 1ml tip (US GCS), 200ul RNase-free PCR reaction tube ...

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PUM

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Abstract

The invention discloses an ultraviolet stress-related gene of highland barley and a cloning method thereof. The gene can be used for detecting whether the highland barley suffers from ultraviolet stress or not. The nucleotide sequence of the ultraviolet stress-related gene of the highland barley provided by the invention is as shown in SEQ ID NO.1. The expression level of the gene is related to whether the highland barley suffers from ultraviolet radiation stress or not, whether the highland barley suffers from ultraviolet radiation stress or not can be judged by detecting the expression level of the gene, theoretical support is provided for the control of subsequent growth conditions of highland barley growth, and the gene has a good market application prospect.

Description

technical field [0001] The invention relates to an ultraviolet stress related gene of barley and application thereof. Background technique [0002] Highland barley English name: hulless barley, is a kind of cereal crops of the Poaceae Barley genus, because the inner and outer glumes are separated and the kernels are exposed, so it is also called naked barley, barley, and rice barley. It is mainly produced in China's Tibet, Qinghai, Sichuan, Yunnan and other places, and is the main food of the Tibetan people. Highland barley has been planted on the Qinghai-Tibet Plateau for about 3,500 years. It has extended from material culture to spiritual culture, forming a highland barley culture with rich connotations and national characteristics on the Qinghai-Tibet Plateau. With a wide range of medicinal and nutritional value, highland barley products such as highland barley noodles, highland barley steamed buns, and highland barley nutritional powder have been launched. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/10C07K14/415C12Q1/686C12Q1/6895
CPCC07K14/415C12Q1/686C12Q1/6895C12Q2600/13C12Q2600/158C12Q2521/107
Inventor 曾兴权原红军徐齐君韦泽秀王玉林扎桑白丽军尼玛扎西
Owner AGRI RES INST TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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