62 results about "South american" patented technology

A cargo transportation system comprising secure (fully closeable and lockable), sealable, stackable, trackable, universal, auto-latching "pallet boxes" that may be used in two main ways: 1) secured to the undercarriage transverse I-beams of over-the-road semi-trailers by means of loading alignment and hanger assemblies that provide positive locking-in-place; and 2) stackable, up to three or more high, inside the trailer bodies. The inventive pallet boxes are designed to be secured by auto-latching hanger assemblies that are retrofittable on individual types of semi-trailer undercarriages, both US and metric (European, Central and South American, and Asian) standard dimensions. The pallet boxes include vertical sidewall load-bearing channel members permitting stacking fully-loaded pallet boxes three or more high. The pallet boxes may be refrigerated, insulated, or atmospherically controlled, e.g., chargeable with preservative, security or disinfecting gases, during shipping.

The invention discloses a bacteriophage for preventing and treating a prawn vibrio parahaemolyticus disease and an expanding culture method thereof. Vibrio parahaemolyticus infected by South American white prawn are used for successfully screening out a strong lytic bacteriophage in seawater, titer of the bacteriophage is up to 1010 to 1012pfu / mL, fermentation period is 8 to 12h, treatment effects are obvious, and the bacteriophage for preventing and treating the prawn vibrio parahaemolyticus disease is of great significance for treatment of diseases causes by the vibrio parahaemolyticus and development of bacteriophage microecological preparations. A storage unit code of the bacteriophage is China Center for Type Culture Collection, address is China Center for Type Culture Collection in Wuhan University Campus, Wuchang Luojia Mountain, Wuhan City, preserved date is December 9, 2016, preservation number is CCTCC NO: M 2016740, a preservation test result is survival, name is vibrio parahaemolyticus bacteriophage VP11, and classification is named as Vibrio parahaemolyticus bacteriophage VP11.

A cargo transportation system comprising secure (fully closeable and lockable), sealable, stackable, trackable, universal, auto-latching “pallet boxes” that may be used in two main ways: 1) secured to the undercarriage transverse I-beams of over-the-road semi-trailers by means of loading alignment and hanger assemblies that provide positive locking-in-place; and 2) stackable, up to three or more high, inside the trailer bodies. The inventive pallet boxes are designed to be secured by auto-latching hanger assemblies that are retrofittable on individual types of semi-trailer undercarriages, both US and metric (European, Central and South American, and Asian) standard dimensions. The pallet boxes include vertical sidewall load-bearing channel members permitting stacking fully-loaded pallet boxes three or more high. The pallet boxes may be refrigerated, insulated, or atmospherically controlled, e.g., chargeable with preservative, security or disinfecting gases, during shipping.

Soybean rust occurs in many countries throughout Asia, Australia, Africa, and South America. The causal agents of soybean rust are two closely related fungi, Phakopsora pachyrhizi and P. meibomiae, which are differentiated based upon morphological characteristics of the telia. Determination of the nucleotide sequence of the internal transcribed spacer (ITS) region revealed greater than 99% / 95% nucleotide sequence similarity among isolates of either P. pachyrhizi or P. meibomiae, but only 80% sequence similarity between the two species. Utilizing differences within the ITS region, four sets of PCR primers were designed specifically for P. pachyrhizi, and two sets of PCR primers were made specific to P. meibomiae. Classical and real-time fluorescent PCR assays were developed to identify and differentiate between P. pachyrhizi and P. meibomiae.

An ecologic reproduction method for the South-American white prawn features that a lighting method is used to culture parent sprawns. After the illuminance is increased, the water quality is improved and the algae can grow on the inner surface of culturing pool for meeting the requirement of parent prawns.

Disclosed is an environment-friendly type compound feed for South American prawn which comprises the following constituents (by weight ratio): red fish meal 15-25kg, soybean meal 10-20g, peanut bran 10-15kg, vegetable seed waste 3-7kg, meat and bones meal 5-10kg, squid internal organ powder 3-8kg, high grade flour 18-25kg, soybean lecithin 1.0-2.0kg, sea fish oil 2.0-3.0kg, choline chloride 0.3-0.6kg, vitamin C and phosphoric ester 0.05-0.1kg, immunopotentiator 0.1-0.2kg, calcium biphosphate 0.5-1.0kg, composite vitamins 0.2-0.5kg, and composite mineral salts 0.3-0.7kg.

The invention discloses a high-efficiency ecological breeding method for South American white shrimps. The high-efficiency ecological breeding method includes the steps such as breeding greenhouse building, offspring seed selection, stocking preparation, breeding management, water quality management. The technology is advanced and applicable; operability is strong; and breeding risk of South American white shrimps is reduced. Disease incidence is reduced. Dissolved oxygen of varied water layers in greenhouses is improved; condition of bottom layer water is improved; improvement in appetites of the South American white shrimps is benefited; and digestibility and utilization rate of feeds are increased.

The invention provides a South American fruit Borojo active extract and an identification and preparation method, a content measuring method and application thereof. The Borojo active extract has natural anti-oxidation, antiracial and antifungal effects.

The invention provides a method for efficiently breeding South American white shrimps. The method comprises the following steps that firstly, at the desalination stage, shrimp fry conveyed from Haian Province are conveyed to a mud pool to be desalinated for 7-8 days, the temperature is controlled between 17 DEG C and 35 DEG C, the aeration is carried out through aerators for 2.5-4.5 hours, at the moment, bittern is poured into the mud pool, and the salinity of the mud pool is desalinated by using fresh water every day, wherein the length of the white shrimps ranges from 5 centimeters to 6 centimeters; secondly, at the breeding stage, the shrimp fry are placed in a pond with the area ranging from 8 mu to 15 mu to be bred for 3 months, the stocking density of the shrimp fry ranges from 120 thousand fry per mu from 15 thousand dry per mu, the transparency of the pond ranges from 20 centimeters to 30 centimeters, the pH ranges from 7.9 to 8.3, the two or three aerators are used for aerating, and feeding fodder is added in the process of breeding and is mixed with vitamins and probiotics promoting substances. The method has the advantages that the rate of survival of the South America white shrimps and the nutritive value of the white shrimps are improved, the growth of an effective microbial community in a water body is promoted, the improvement of the water quality is promoted, and eutrophication caused by unnecessary fodder is avoided.

The invention relates to a special feed additive for a South American white shrimp. The special feed additive is prepared from the following raw materials by weight: 5 to 10 parts of perilla, 5 to 8 parts of Chinese honey locust, 5 to 7 parts of green tangerine peel, 2 to 4 parts of cassia twig, 2 to 3 parts of aniseed, 3 to 7 parts of white peony root, 0.02 part of vitamin C, 0.001 part of vitamin K and 20 to 25 parts of a carrier. 0.5 to 1.2% of the special feed additive for the South American white shrimp provided by the invention is added into a conventional mixed feed for the shrimp for breeding of the South American white shrimp. With the special feed additive for the South American white shrimp provided by the invention, economic benefits in breeding of the shrimp are obviously improved, occurrence of diseases during breeding of the shrimp is substantially reduced, the survival rate of the shrimp is increased by 19 to 22%, growth of the shrimp is promoted, and quality of the shrimp is enhanced.

The invention discloses a method which can rapidly identify liriomyza approximate species by PCR-RFLP technology, which belongs to the insect species identification technical field. The method includes: (1) the DNA extraction of liriomyza trifolii, Americanliriomyza and South American liriomyza samples; (2) obtaining DNA sequence of 805bp by the PCR reaction of COII gene according to insect COII gene universal primers; (3) obtaining the COII sequence respectively through the clone, the transformation and the sequencing; (4) the selection of restriction endonuclease DraI; (5) the distinction of three approximate liriomyzas through a restriction map. The invention has the characteristics of accuracy, stability, fastness, cheapness, etc. And the invention can be applied in related departments of quarantine inspection, forecast, prevention, etc. to realize the rapid detection on the three approximate liriomyzas.

The invention discloses a South American wedelia chinensis antioxidant extract as well as a preparation method and application of extract. The extract is extracted from dry powder of South American wedelia chinensis by adding a solvent; extracting solution is subjected to filtration and reduced pressure concentration to obtain total extract, extraction is carried out with petroleum ether and ethylacetate sequentially, reduced pressure recycling is carried out to obtain a petroleum ether part, an ethyl acetate part and a water dissolving part respectively, the obtained ethyl acetate part and water dissolving part respectively have excellent DPPH free radical scavenging activity and oil oxidation resistant activity, and the ethyl acetate part contains nine fiavonoids and four penylpropanoids. The obtained extract is high in yield, technological process is simple, and the extract is applicable to relatively large-scale preparation. The obtained extract has excellent antioxidant activityand can be applied to antioxidant drugs and food preservation as active components.

The invention aims to provide an aquaculture process for South American eel larvae. The aquaculture process includes cleaning aquaculture ponds, and controlling the water temperatures and the salinity of the eel fry ponds. The aquaculture ponds are soaked or cleaned by the aid of dry quicklime powder, bleaching powder, potassium permanganate, clear water and the like; the water temperatures and the salinity of the aquaculture ponds are controlled in such modes that temperature difference of the ground are regulated, water is blended, salt is added into the water at specified steps, the water is diluted at specified steps and the like. The aquaculture process has the advantages that cleaning optimization schemes implemented before the aquaculture ponds are utilized are optimized by means of exploration, and methods for removing cement alkali are improved; aquaculture pond sterilization modes are optimized; the growth speeds of the eel larvae are increased, eel deformity can be prevented, and the death rates in larva aquaculture periods can be decreased; eel larva releasing modes and salinity regulation modes are modified, and accordingly death of the eel larvae due to temperature or salinity problems in releasing procedures further can be reduced.

The present invention discloses 4-nerolidylcatechol and its derivatives isolated from South American / Amazon plants (Pothomorphe species) and their potential use as therapeutical agent for treatment of malarial symptoms, including malarial patients resistant to traditional drugs. The present invention also discloses a method for producing 4-nerolidylcatechol and their derivatives.

The present invention provides isoformes from a peptide family belonging to South American scorpion Tityus serrulatus that acts as hypotensive agents by potentiating Bradykinin and, therefore, can be used as anti-hypertensive drugs. A peptide was firstly isolated from Tityus serrulatus venom and showed a strong and long-lasted hypotensive activity when tested in rats. This peptide was first named TsHpt-I (Tityus serrulatus Hypotensin-I). Also, three more highly similar isoformes were identified and revealed a peptide family with very close primary structure. They were named TsHpt-II, TsHpt-III and TsHpt-IV.

The invention belongs to the technical field of natural pharmaceutical chemistry, and relates to Borojo acid 1 to Borojo acid 13 separated from South American fruit, namely Borojo, and a preparation method and application of the Borojo acid 1 to Borojo acid 13. The preparation method comprises the steps that Borojo mature fruits serve as raw materials, and are peeled and subjected to seed removing, water is added into pulp, even mixing and grinding into thick liquid are conducted, or water is added into Borojo freeze-dried powder, even mixing and filtering are conducted, ethyl acetate is addedinto a filtrate for extraction, an ethyl acetate layer is concentrated to be dry and then subjected to chromatographic separation technologies such as silicone, octadecylsilane chemically bonded silica (ODS), sephadex LH-20 and preparative high performance liquid chromatography (Pre-HPLC), and thirteen Borojo acid compounds are obtained through separation from the Borojo, wherein the Borojo acid1 to the Borojo acid 10 are new compounds, and the other three Borojo acid compounds are known compounds obtained through first separation from the Borojo. Experiments show that the prepared compoundshave activities of resisting oxidation, resisting inflammation and reducing blood glucose.

The invention discloses a bacteriophage for preventing and treating a prawn vibrio parahaemolyticus disease and an expanding culture method thereof. Vibrio parahaemolyticus infected by South American white prawn are used for successfully screening out a strong lytic bacteriophage in seawater, titer of the bacteriophage is up to 1010 to 1012pfu / mL, fermentation period is 8 to 12h, treatment effects are obvious, and the bacteriophage for preventing and treating the prawn vibrio parahaemolyticus disease is of great significance for treatment of diseases causes by the vibrio parahaemolyticus and development of bacteriophage microecological preparations. A storage unit code of the bacteriophage is China Center for Type Culture Collection, address is China Center for Type Culture Collection in Wuhan University Campus, Wuchang Luojia Mountain, Wuhan City, preserved date is December 9, 2016, preservation number is CCTCC NO: M 2016740, a preservation test result is survival, name is vibrio parahaemolyticus bacteriophage VP11, and classification is named as Vibrio parahaemolyticus bacteriophage VP11.

The invention discloses a high-efficiency ecological breeding method for South American white shrimps. The high-efficiency ecological breeding method includes the steps such as breeding greenhouse building, offspring seed selection, stocking preparation, breeding management, water quality management. The technology is advanced and applicable; operability is strong; and breeding risk of South American white shrimps is reduced. Disease incidence is reduced. Dissolved oxygen of varied water layers in greenhouses is improved; condition of bottom layer water is improved; improvement in appetites of the South American white shrimps is benefited; and digestibility and utilization rate of feeds are increased.

The invention discloses a South American white prawn shrimp transport device, and belongs to the technical field of aquatic product transportation. The transport device comprises a transport barrel, the outer wall of the transport barrel is connected with a bracket, the bottom end of the bracket is connected with a bottom plate for supporting the transport barrel, the inner wall of the transport barrel is symmetrically connected with box body sliding channels, the box body sliding channels are connected with transport boxes, sliding channel holders fixedly cooperated with the box body slidingchannels are connected to both sides of the tops of the transport boxes, and the upper ends of the transport boxes are connected with runners; the transport device is less affected by the external environment, the practical use is high in flexibility, aquaculture water circulation is achieved, damage to South American white prawn shrimps is small, transport survival rate is high, and the molting cycle and recovery time of the South American white prawn shrimps are reduced.

The invention discloses special seed rearing bait for South American white shrimps. The special seed rearing bait comprises, by weight, 10-14 parts of diatom, 10-14 parts of platymonas, 10-14 parts of dunaliella, 5-8 parts of spirogyra, 5-8 parts of ulva pertusa, 15-20 parts of oyster meat, 16-18 parts of eggs, 20-30 parts of daphnia, 25-30 parts of rotifer, 28-34 parts of artemia and 30-40 parts of fairy shrimps. The special seed rearing bait for shrimps has the advantages that the survival rate of brine shrimp seeds can be effectively increased, and the special seed rearing bait is high in palatability and applicable to large-scale production and contains abundant nutrition; the survival rate of the brine shrimp seeds can be increased by 5%-10% as compared with existing products, and accordingly the special seed rearing bait has an excellent market prospect.

The invention relates to a method for off-season industrial culture of Penaeus vannamei, which comprises the following steps: (1) site selection of the farm, design of the culture workshop and shrimp pond, (2) preparation before releasing seedlings, (3) temporary cultivation of seedlings , (4) management during the cultivation period and (5) harvest. The method of the present invention can carry out off-season cultivation of Penaeus vannamei in northern regions, and is not limited by seasons and temperatures compared with traditional cultivation, and can control environmental conditions such as temperature, light, and dissolved oxygen in the optimum growth environment of Penaeus vannamei, It can prevent the cross-infection of pathogenic microorganisms, reduce the occurrence of diseases, high-density intensive cultivation, and the yield is obviously improved, avoiding the period when the prawns are concentrated on the market, adjusting the market supply, and helping to increase farmers' income. At the same time, the method of the invention effectively utilizes the idle time in winter Breeding has greatly improved the utilization rate of land resources.

The present invention provides isoformes from a peptide family belonging to South American scorpion Tityus serrulatus that acts as hypotensive agents by potentiating Bradykinin and, therefore, can be used as anti-hypertensive drugs. A peptide was firstly isolated from Tityus serrulatus venom and showed a strong and long-lasted hypotensive activity when tested in rats. This peptide was first named TsHpt-I (Tityus serrulatus Hypotensin-I). Also, three more highly similar isoformes were identified and revealed a peptide family with very close primary structure. They were named TsHpt-II, TsHpt-III and TsHpt-IV.

A technique for preparing the between-meal eatable from South-American white prawn includes such steps as thawing, pre-treating, preparing flavouring, putting them in a container, adding water, boiling, dripdrying, cooling, packing and sterilizing.

The invention provides protein feed derived from rhodopseudomonas palustris and a preparation method and application of the protein feed. The protein feed is prepared from the following components inparts by weight: 90-93 parts of a broken wall thallus, 2-3 parts of lysine hydrochloride, 0.1-0.5 part of methionine, 0.1-0.5 part of threonine, 0.3-1 part of arginine, 2-3 parts of calcium hydrophosphate and 1-2 parts of calcium carbonate, wherein the broken wall thallus is the rhodopseudomonas palustris; and the preparation method includes the steps of thallus collection, lysozyme pretreatment,thallus wall breaking and formula combination, the wall breaking rate of a rhodopseudomonas palustris thallus reaches 100% by adopting the preparation method, the amino acid ratio is ideal, and the animal absorption use ratio is high. The invention further discloses application of the protein feed derived from the rhodopseudomonas palustris in duroc*(large white*landrace) ternary miscellaneous piglets, Qingyuan local pullets, carassius auratus gibelio and South American white shrimps. The protein feed is used for replacing corresponding fish meal, animal growth performance is better or not affected, and the protein feed is ideal.

The invention relates to an extraction method for a South American wedelia chinensis total ribonucleic acid (RNA) and belongs to the technical field of biology. The method includes grinding a fresh South American wedelia chinensis material to be fine powders by liquid nitrogen, performing a warm bath at the temperature of 65DEG C for 30 minutes, adding equivoluminal chloroforms and evenly mixing, centrifuging at the low temperature of 4DEG C, subjecting an obtained mixture to a precipitation by adding a lithum chloride solution, centrifuging, abandoning supernatant, dissolving the precipitation by tellurium, adding equivoluminal chloroforms, centrifuging, extracting the supernatant to a new centrifuge tube, adding diethyl ether, centrifuging, extracting the supernatant, repeating a diethyl ether operation for two times, adding natrium aceticum and absolute ethyl alcohol for precipitating the RNA, centrifuging, abandoning supernatant, washing the RNA by 70% of ethanol to obtain the precipitation, drying the obtained precipitation at the room temperature, dissolving by aseptic diethylpyrocarbonate (DEPC) water, and preserving for a reservation at the temperature of 80 DEG C below zero. According to the extraction method for the South American wedelia chinensis total ribonucleic acid (RNA), the experiment processes are simple, the demands for equipment and experiment conditions are low, the result is accurate, effective, the repeatability is good, and the method has an important guiding and practical significance for extracting the total RNA of plant tissues which are rich in phenols and polysaccharides.

The invention provides an indoor small water body cultivating method for South American white shrimps at temporary cultivation stage for thickening. The method comprises following steps: S1. disinfecting a container and water body, wherein the water carrying capacity of the container is 0.1 to 0.5 ton; S2. selecting healthy shrimp seeds; S3. temporary cultivating for thickening: the cultivating period is 20 to 30 days, water is not changed at the first eight days during cultivation, and EM bacterium is spread in the container every other day, after the first eight days, water is changed every day and EM bacterium is spread after water changing; shading treatment is carried out at the first eight days and cultivation under natural light is carried out after the first eight days; during the cultivation process, feeding for 12 times at schedule time is carried out daily and the intervals of 90 to 120 minutes are between each feeding; 3 to 5 days before the end of the temporary cultivating for thickening, vitamin C solution is spread daily for strengthened treatment for the shrimp seeds. The survival rate of shrimp seeds obtained by the temporary cultivating method for thickening is high and the growth is fast.

The invention relates to the technical field of accessory devices for cleaning and fishiness removing of South American white prawns, and in particular to a device for processing, cleaning and fishiness removing of the South American white prawns. The device has the beneficial effects that fishiness removing liquid can be recycled, so that the resource is saved and the use reliability is increased; in the meanwhile, the clean effect can be increased and the water resource is saved, so that the usability is enhanced; and the drying speed of the South American white prawns is increased, so thatthe use effect is improved. The device comprises a base, a water groove, a bracket, a water inlet pipe, an input pipe, an output pipe, a mesh plate, a processing box, a discharge pipe, a feed pipe, afixing frame, an infusion pump, a communication pipe, an adjusting frame, a shunt pipe, multiple groups of nozzles, a first auxiliary member, a first electric heating net, a second auxiliary member and a second electric heating net, wherein a control valve is arranged on the output pipe; the infusion pump is arranged on the fixing frame; a plurality of shunt branch pipes are arranged on the outputend at the bottom of the shunt pipe; the second auxiliary member and the second electric heating net are both arranged at a second mounting opening; and the second electric heating net is arranged atthe left side of the second auxiliary member.

The invention relates to a breeding method for bionic freshwater South American parent white shrimps, and belongs to the technical field of parent shrimp breeding methods. The method includes the following steps: allowing the parent shrimps to lay eggs in seawater, and hatching the eggs; gradually desalting a water body for culturing young shrimps until the salinity of the water body is not higherthan 1.5%o, and culturing the young shrimps into sub-adult shrimps under normal production conditions; gradually salting a water body for culturing the sub-adult shrimps until the salinity of the water body reaches 28-35%o; taking the sub-adult shrimps as standby parent shrimps for continuous culture; selecting the standby parent shrimps free of broken limbs and full of vigor as candidate parentshrimps. Compared with conventional breeding methods for South American white shrimps, the breeding method has the advantages that the bred South American white shrimps can adapt to various breeding environments to ensure high survival rate, high growth rapid and high stress resistance in the cultivation process of the South American white shrimps; the reproductive properties of the South Americanwhite shrimps can be maintained, and the obtained desalted or salted shrimps can be taken as the parent shrimps and have high fertility.

The invention relates to a Chinese herbal medicine micro-ecological preparation for resisting transport stress in Penaeus vannamei, which is prepared from the following raw materials in parts by weight: 22-26 parts of gallnut, 14-21 parts of poplar flower, 5-20 parts of gentian, Cen 10-15 parts, Wujiapi 5-10 parts, fermentation strains and liquid culture medium. The preparation is obtained by fermenting the raw materials of Chinese herbal medicine in a liquid culture medium through fermentation strains. The beneficial effects of the present invention are as follows: 1. The raw material components of the compound Chinese herbal medicine have wide sources, low cost, simple preparation, and significant effects; 2. The fermentation strain is a beneficial bacterial strain capable of decomposing and producing antioxidant substances, and can release a large amount of active ingredients of the Chinese herbal medicine , and can improve shrimp disease control and intestinal health to a certain extent; 3. It can significantly reduce the stress red body phenomenon that occurs during the transportation of prawns; 4. It has obvious economic value when applied to Penaeus vannamei. The survival rate of transportation has been significantly improved.