The present invention relates to a sterilization enzyme-inactivating method for fluid foods. Said fluid foods includes liquid or semi-solid foods, such as fresh milk, watermelon juice, apple juice, orange juice, tomato juice, strawberry juice, vegetable juice, pulpy fruit juice and vegetable paste, etc. It is characterized by that said invention adopts combination of pasteurization and high-voltage pulse electric field to make sterilization and inactivate enzyme. Its electric field intensity is 75-50 kV / cm.