65 results about "Schistosoma sinensium" patented technology

The invention discloses a Wnt family gene which is amplified for the first time from Schistosoma japonicum 19-day schistosomulum by utilization of RACE technology, wherein, sequence analysis indicates that: a complete coding frame of the gene comprises 1311bp, 436 amino acids coded; and theoretical molecular weight of 49.6kD. Homology analysis results indicate that: amino acid sequences of the gene have typical Wnt family protein characteristics; similarity of the amino acid sequences with amino acid sequences of Dugesia japonica and human Wnt4 is relatively 43 percent and 37 percent; the gene is presumed to be a Wnt4 gene of schistosome and named as Sjwnt4 (GenBanK Log-On No.: DQ643829). Real-time quantitative PCR analysis indicates that: the gene is expressed all in 14-day schistosomulum, 19-day schistosomulum, 31-day imago, 44-day female worms and 44-day male worms. The invention constructs a pronucleus expression vector pGEX-4T-2-Sjwnt4 of the gene and applies an Escherichia colisystem for expression; expression proteins exist in the form of inclusion bodies; Western blottings indicate that expression products can be identified by crude antigen immune serums of Schistosoma japonicum imago.

The invention discloses a Schistosoma japonicum recombinant antigen, which is produced by expressing a recombinant vector containing a Schistosoma japonicum MRP1 gene fragment. The sequence of the MRP1 gene fragment encodes the amino acid sequence shown in SEQ ID NO.1 Nucleotide sequence. The invention also discloses the application of the above-mentioned schistosomiasis recombinant antigen in the preparation of products for diagnosing schistosomiasis japonicum. The schistosomiasis recombinant antigen rSjMRP1 of the invention is used for diagnosing schistosomiasis, has high sensitivity and strong specificity, and is of great significance for the prevention and treatment of schistosomiasis.