siRNA of schistosoma japonicum SjELAV-like 2 genes and application thereof
A technology for schistosomiasis and schistosomiasis, applied in the fields of molecular biology and biomedicine, can solve the problems of low meiotic efficiency of mature oocytes and other problems
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Embodiment 1
[0028] Example 1 In vivo RNA interference screening for optimal dsRNA
[0029] 1. Method steps
[0030] 1.1 siRNA molecules and SjELAV-like 2 Preparation of Gene Real-time Quantitative PCR Primers
[0031] Schistosoma SjELAV-like 2 The gene sequence (MG515726) was analyzed and compared, and three pairs of dsRNA were designed and synthesized. The principles of siRNA design are as follows:
[0032] 1. Starting from the AUG start codon of the transcript (mRNA), look for the "AA or NA" double sequence, and write down the 19 nucleotide sequence at its 3' end as a potential siRNA target site. Both sense and antisense strands were designed using these 19 bases (excluding AA or NA repeats).
[0033] 2. Avoid selecting sequences of interest near start codons or nonsense regions.
[0034] 3. The GC content of the siRNA sequence should be around 30%-60%.
[0035] 4. When designing siRNA, do not target the 5' and 3' non-translated regions (untranslated regions, UTRs), because ...
Embodiment 2
[0058] Example 2 Long-term RNA interference in vivo
[0059] 1. Method steps
[0060] 1.1 dsRNA injection and worm collection
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