Method for obtaining nilaparvata lugens new strains stably infected with Cardinium by artificial transfection
A technology of brown planthopper and a new strain, applied in the biological field, can solve the problems of inability to transfect new hosts, low infection rate, and inability to stably infect
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[0062] The following examples are further descriptions of the present invention, rather than limitations of the present invention.
[0063] (1) Collection of planthopper eggs
[0064] 30 mature brown planthoppers infected with Cardinium and 30 mature brown planthoppers not infected with symbiotic bacteria were respectively taken to lay eggs in two cups of rice seedlings for 4-5 hours.
[0065] Place the oviposited rice seedlings on a clean petri dish, remove the roots and leaves, carefully dissect the seedlings under a stereomicroscope with dissecting tweezers, and then transfer their eggs to two pieces of wet filter paper, These embryos were then placed on nitrocellulose membranes and transferred to glass slides fixed with double-sided tape.
[0066] The dissected eggs of white-backed planthopper and brown planthopper were used as donors and recipients of artificial transfection, among which eggs of white-backed planthopper infected with Cardinium were used as donors of arti...
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