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Method for producing conidia through liquid fermentation of Sphaeria sinensis

A technology of Cordyceps sinensis and conidia, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of low concentration of conidia, achieve short cultivation period, and cheap and easy-to-obtain raw materials , the effect of stabilizing infectivity

Pending Publication Date: 2021-10-29
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that liquid fermentation Cordyceps sinensis produces conidia, to solve the problem that the conidia concentration is low during the liquid fermentation of Cordyceps sinensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 liquid fermentation Cordyceps sinensis produces conidia steps as follows:

[0019] 1 Cordyceps sinensis strain activation:

[0020] Cordyceps sinensis was inoculated on potato slant medium and cultured for 5 days in a dark environment at a temperature of 12°C.

[0021] 2 liquid seed culture:

[0022] Cut the cultured slant strains into the size of broad beans, put them in the liquid seed medium, put 100mL inoculum into a 250mL Erlenmeyer flask, and place them in a constant temperature shaker for 30 days, the shaker speed is 150r / min, and the culture temperature is 12°C . The preparation formula of the liquid seed medium is: 30g of potato powder, 10g of glucose, 40g of maltose, 10g of peptone, 10g of yeast powder, 1.5g of magnesium sulfate, 3g of potassium dihydrogen phosphate, 0.5g of triamine citrate, and 1000mL of water.

[0023] 3 conidia culture

[0024] The cultured Cordyceps sinensis liquid seeds were inoculated into the liquid conidium medium at ...

Embodiment 2

[0027] Embodiment 2 liquid fermentation Cordyceps sinensis produces conidia steps as follows:

[0028] Step 1 is the same as in Example 1.

[0029] 2 liquid seed culture:

[0030] Cut the cultured slant strains into the size of broad beans, put them in the liquid seed medium, put 100mL inoculum into a 250mL Erlenmeyer flask, and place them in a constant temperature shaker for 30 days, the shaker speed is 150r / min, and the culture temperature is 12°C . The preparation formula of the liquid seed medium is: 15g of potato dipping powder, 5g of glucose, 20g of maltose, 5g of peptone, 5g of yeast powder, 1g of magnesium sulfate, 1.5g of potassium dihydrogen phosphate, 0.3g of triamine citrate, and 1000mL of water.

[0031] 3 conidia culture

[0032] The cultured Cordyceps sinensis liquid seeds were inoculated into the liquid conidium medium at an inoculum size of 10%, and 100mL of the inoculum was packed into a 250mL Erlenmeyer flask, placed in a constant temperature shaker for 3...

Embodiment 3

[0035] Embodiment 3 liquid fermentation Cordyceps sinensis produces conidia steps as follows:

[0036] Step 1 is the same as in Example 1.

[0037] 2 liquid seed culture:

[0038] Cut the cultured slant strains into the size of broad beans, put them in the liquid seed medium, put 100mL inoculum into a 250mL Erlenmeyer flask, and place them in a constant temperature shaker for 30 days, the shaker speed is 150r / min, and the culture temperature is 12°C . The preparation formula of the liquid seed medium is: 30g of potato powder, 10g of glucose, 40g of maltose, 10g of peptone, 10g of yeast powder, 1.5g of magnesium sulfate, 3g of potassium dihydrogen phosphate, and 1000mL of water.

[0039] 3 conidia culture

[0040] The cultured Cordyceps sinensis liquid seeds were inoculated into the liquid conidium medium at an inoculum size of 10%, and 100mL of the inoculum was packed into a 250mL Erlenmeyer flask, placed in a constant temperature shaker for 30 days, and the shaker speed wa...

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Abstract

The invention belongs to the technical field of microbial fermentation, and particularly discloses a method for producing conidia through liquid fermentation of Sphaeria sinensis, the method comprises the steps of Sphaeria sinensis activation, liquid seed culture and conidia culture, and a liquid seed culture medium and a liquid conidia culture medium are used; the fermentation concentration is high, the produced conidia can reach 3.5*10<6> cfu / mL to the maximum, the raw materials are cheap and easy to obtain, the cost is low, the culture period of the produced conidia is short, and the problem that the conidia concentration is low when Sphaeria sinensis is subjected to liquid fermentation is solved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and specifically discloses a method for producing conidia by liquid fermentation of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a precious Chinese medicinal material unique to the Qinghai-Tibet Plateau in my country. It has the functions of sedation, hemostasis, lowering blood pressure, improving myocardial ischemia, anti-platelet aggregation, anti-aging, anti-tumor and two-way regulation of human immunity. But natural Cordyceps sinensis has its strict parasitic and special growth environment, low yield and high price. The growth conditions of natural Cordyceps sinensis are extremely special, coupled with global climate change, ecological environment destruction, and predatory excavation, the wild resources of Cordyceps sinensis are decreasing day by day, the drug source is in short supply, and the price is expensive. In order to change this situation, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N3/00C12N1/14
Inventor 邢康康陈仕江贺元川张德利贺宗毅游华建罗昌树鲁增辉涂永勤石萍谭发银卫秋阳邓小书
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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