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Production method for euproctis pseudoconspersa nuclear polyhedrosis virus

A nuclear polyhedron and production method technology, applied in the production field of tea caterpillar nuclear polyhedrosis virus, can solve the problems of affecting the feeding amount of larvae, fragile virus carcasses, and death of overwintering eggs, so as to reduce the infection of tea caterpillar virus The probability of hair growth, the effect of ensuring the number of larvae feeding, and ensuring the amount of virus production

Active Publication Date: 2014-02-19
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, the virus yield is not high
Because tea caterpillar egg masses survive the winter for as long as 4-5 months, the leaves of hydroponic tea branches often fall and wither, resulting in the death of a large number of overwintering eggs, and the hatching rate of eggs is lower than 15%, which affects the feeding amount of the first generation larvae
At the same time, in the process of virus production and collection of dead insects, because the virus corpses are easily broken, there will be more losses during collection, and the virus yield per unit area will be low, thus affecting the virus production.

Method used

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  • Production method for euproctis pseudoconspersa nuclear polyhedrosis virus

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Experimental program
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Effect test

Embodiment 1

[0019] Put the potted tea tree into an insect cage (length 55cm×width 55cm×height 90cm, plastic gauze), insert 100-150 pairs of adult tea caterpillars, let them mate in the cage, and lay eggs on the back of the leaves. The insect cages are placed outdoors or indoors at natural temperature for overwintering.

[0020] After the eggs hatch, cut off the leaves with worms and put them in canning jars. One egg mass per bottle is added to several pieces of old leaves for feeding, and the mouth is tied with paper, and the cover glass is used to keep moisture. When most of the leaves in the bottle are eaten, remove the debris in time and add new leaves. When the larvae grow to the 4th instar, move the larvae in the bottle to a wooden box, about 1000-1500 worms per box, add tea branches with old leaves to raise, and cover the box with a wet towel and glass to moisturize.

[0021] When the larvae grow to the middle stage of the 6th instar, the poisoning treatment is carried out. The m...

Embodiment 2

[0025] After the adults start to emerge, put the canned bottle containing the tea caterpillar pupae into the insect cage (30cm×46cm×55cm, plastic gauze), open the paper cover of the bottle mouth, 3 bottles per cage, and place two in the cage at the same time. Bundle the tea branches cultivated in water, let the adults mate and lay eggs.

[0026] After the eggs hatch, cut off the leaves with worms and put them in canning jars. One egg mass per bottle is added to several pieces of old leaves for feeding, and the mouth is tied with paper, and the cover glass is used to keep moisture. When most of the leaves in the bottle are eaten, remove the debris in time and add new leaves. When the larvae grow to the 4th instar, move the larvae in the bottle to a wooden box, about 1000-1500 worms per box, add tea branches with old leaves to raise, and cover the box with a wet towel and glass to moisturize.

[0027] When the larvae grow to the middle stage of the 6th instar, the poisoning tr...

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Abstract

A production method for euproctis pseudoconspersa nuclear polyhedrosis virus belongs to the technical field of microbial pesticide production. The production method comprises the following technological steps: 1) large-scale breeding of larva; 2) virus propagation; 3) virus collection; and 4) virus extraction. The production method comprises the following beneficial effects: firstly, skin allergy caused by toxic hair of euproctis pseudoconspersa is prevented, because the probability of euproctis pseudoconspersa toxic hair contamination is substantially reduced by exchanging forage in a fume cupboard and collecting euproctis pseudoconspersa dead bodies, and allergy is effectively avoided; secondly, the virus production yield is improved, by employing potted tea trees for producing overwintering spawns, the hatching rate of the overwintering spawns is substantially improved from 10-15% to 65-80%, so that first-generation larva breeding number and virus productivity in next year are ensured; and also, the virus dried dead body collection method is employed, helps to reduce the loss of virus during collection, and is improved in efficiency by 52.7% and in virus productivity by 38.8% compared with a conventional collection method.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticide production, and in particular relates to a production method of tea caterpillar nuclear polyhedrosis virus. Background technique [0002] tea caterpillar ( Euproctis pseudoconspersa ) is the main leaf-eating pest in my country's tea gardens. It not only affects the yield of tea, but also easily causes human skin allergies due to the poisonous hairs on the insects, seriously affecting the field management of tea gardens. Tea caterpillar nuclear polyhedrosis virus ( Euproctis pseudoconspersa nucleopolyhedrovirus (EpNPV for short) is a dominant species of natural enemies of tea caterpillars, which has the advantages of good insecticidal effect on tea caterpillars, natural enemies of tea gardens and environmental safety. The production of tea caterpillar nuclear polyhedrosis virus adopts live worm proliferation method, and its production process is as follows: firstly, a large number of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 唐美君殷坤山郭华伟肖强
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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