The invention provides an
antibody biological activity detection method based on a surface
plasma resonance technology, and particularly relates to the technical field of biological detection, human Burkitt's
lymphoma cell strain Daudi cells highly expressed by an
LAG3
receptor are selected to be combined with a C1
chip, and the combined cells are fixed on the surface of the
chip; when the
LAG3 iscombined with cells on the surface of the
chip, a response
signal is captured through a biomacromolecule interaction instrument Biacore T200; when the anti-
LAG3
antibody is added, the combination ofLAG3 and cells is reduced, the response value is reduced, and the
antibody activity at the
cellular level is realized by comparing the response value so as to realize
rapid detection. The method is simple in step, easy to operate, capable of achieving real-time
dynamic monitoring and high in efficiency, has a specific recognition effect, and has great advantages in antibody
drug screening, batch release, stability monitoring, even
process development and other applications of
immune checkpoint targets.