40 results about "Glutathione synthesis" patented technology

Nutritional or therapeutic compositions containing glutamic acid, cystine, glycine and a selenium precursor and methods for their utilization to increase glutathione synthesis and thereby enhance the immune system are described.

There is provided a method of reducing insulin resistance in a mammalian patient comprising selecting a patient suffering from insulin resistance and administering a compound which increases hepatic glutathione and a compound which increases hepatic nitric oxide.

Nutritional or therapeutic compositions containing glutamic acid, cystine, glycine and a selenium precursor and methods for their utilization to increase glutathione synthesis and thereby enhance the immune system are described.

A method for promoting the synthesis of glutathione by microbial enzymatic method relates to the technical field of synthesizing high-value useful compounds by using microbial cell enzymatic method. Taking recombinant Escherichia coli enzymatic synthesis of glutathione as an example, the present invention uses cultured recombinant Escherichia coli WSH-KE1 cells as the enzyme source, and directly adds low-concentration organic solvents or surfactants to the reaction system, To reduce the permeability barrier of the outer cell membrane, in the presence of adenosine triphosphate (ATP), catalyze L-Glu, L-Cys and Gly to synthesize glutathione, and the glutathione synthesis amount can reach 4.8g / L after 2 hours of reaction. The invention simplifies the procedure of microbial cell permeability treatment, avoids the possibility of loss of intracellular substances during centrifugation caused by the damage of the permeabilizer to the cell membrane or the lysis of the cells, and ultimately increases the glutathione production.

The present invention provides uses of multi-nutrient supplements to rescue aberrant biochemical pathways and reduce birth defect caused by maternal diabetes. Choice of supplements is based on the ability of each supplement to correct the following hyperglycemia-associated abnormalities: increased reactive oxygen species generation, abnormal membrane phospholipid metabolism, and decreased glutathione synthesis.

Disclosed are methods of treating hyperproliferative disorders such as cancer, methods of arresting the cell cycle in cancer cells, methods of inhibiting glutathione synthesis in cancer cells, and associated compounds for use and uses in medicaments. In certain embodiments, the methods, uses and compounds are provided with reference to compounds of the structural formula (I), in which X1, X2, Z1,Z2, the ring system denoted by "a", R1, L1, L2, Q, L3, R3, L4, R4, L5, and R5 are as described herein. In certain embodiments, compounds disclosed herein are especially active against cancers having amutant KRAS gene.

Topical personal care compositions containing amino acids and 4-substituted resorcinols for potentiating glutathione synthesis within skin cells. The compositions can be used to improve skin appearance of chronological aging or photoaging, resulting from exposure to UV light / sunlight, or environmental pollutants. The compositions are also useful for attaining even skin color and reducing pigmentation, age spots and discoloration.

Glutathione (GSH) is a tripeptide of extreme importance as a catalyst, reductan, and reactant. It can be depleted intracellulary either by forming a direct complex with an electrophilic agent (accomplished investigationally by agents such as bromobenzene or diethyl maleate), by way of inhibition of synthesis, or by subjecting cells to oxidant stress. Most cells, except for epithelia cells, do not have a direct transport capacity for intact GSH. Non-epithelial cells must either transport precursor substrates for GSH synthesis or salvage amino acids from circulating GSH for reuse in intracellular resynthesis. Dietary cysteine is a rate limiting substrate for the synthesis of glutathione and also inhibits GSH efflux. Although GSH is synthesized from precursors in virtually all cells, the liver is the main source of plasma GSH. Protection and support of liver function is paramount to elevating GSH levels. The disclosure is also of a unique combination of nutritional supplements including n-acetyl cysteine, vitamin C, l-glucosamine, n-acetyl d-glucosamine, quercitin, sylimarin, Alpha lipoic acid and high protein, low fat whey that are combined to support various bodily systems involved in glutathione synthesis, reutilization and storage; all intended to elevate glutathione concentration in the mammalian cell.

The invention discloses a series of mutants of bifunctional glutathione synthetase and an application of the mutants in glutathione synthesis. The series of mutants are obtained by single-point mutation or combined mutation on amino acids at 29th, 440th, 442nd, 483rd, 488th, 573rd and 576th sites of bacillus cereus bifunctional glutathione synthetase, wherein activity of the M4 mutant with the highest activity is 26.6 times higher than that of wild glutathione synthetase, glutathione synthesized by the M4 mutant under enzymatic synthesis has the highest concentration reaching 24.8 g / L, whichis 5.76 times higher than that of the wild glutathione synthetase, the production cost is greatly reduced, and the mutants have great application value.

The present invention relates to a method of producing glutathione, wherein photosynthetic membrane vesicles and enzymes catalyzing glutathione synthesis are combined and glutamate, cysteine and glycine are used as reaction substrates. As enzymes catalyzing glutathione synthesis, γ-glutamylcysteine synthetase and glutathione synthetase may be used together, or bifunctional glutathione synthetase may be used alone. According to the conventional methods, there is a problem in that expensive adenosine triphosphate should be continuously supplied when glutathione is produced. However, according to the present invention, since photosynthetic membrane vesicles are used as a source to regenerate adenosine triphosphate, it is possible to continuously produce glutathione without additionally adding adenosine triphosphate, thereby reducing production costs of glutathione.

The invention relates to the field of polypeptide synthesis, in particular to an immobilized fusion enzyme and a method for preparing glutathione with it. In the present invention, the glutathione synthetase gene, the adenosine triphosphate regenerating enzyme gene and the chitin protein domain gene are respectively connected and expressed to prepare a fusion enzyme, and the chitin carrier can be used to specifically and firmly bind the chitin protein domain , realize one-step purification of immobilized glutathione synthesis fusion enzyme and adenosine triphosphate regeneration fusion enzyme, the process is simple, and the space density of immobilized enzyme is high. The immobilized fusion enzyme prepared by the present invention has high enzyme activity and can realize continuous production of glutathione. The present invention adopts the batch and continuous process route of the immobilized fusion enzyme to generate glutathione up to 31g / L. The target The product concentration is high, and there is no enzyme residue in the product solution, which greatly simplifies the later purification process.

Disclosed are compounds useful, for example, in methods of treating hyperproliferative disorders such as cancer, methods of arresting the cell cycle in cancer cells, methods of inhibiting glutathione synthesis in cancer cells, and associated compounds for use and uses in medicaments. In certain embodiments, the methods, uses and compounds are provided with reference to compounds of the structural formulae (Ia), (Ib), (Ic), (Id) and (Ie), in which R1, L1, L2, Q, L3, R3, L4, R4, L5, and R5 are as described herein. In certain embodiments, compounds disclosed herein are especially active against cancers having a mutant KRAS gene.

The invention relates to a method for culturing selenium-enrichment and glutathione-enrichment cucumbers. The method includes the steps that 1, when cucumber seedlings are cultured, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are adopted, the seedling culturing density is 150 to 200 per square meter, and the applied fertilizer quantity ranges from 8 kg per mu to 10 kg per mu; 2, the seedlings which are obtained in the first step of seedling culturing and each have three to four main leaves are planted, and before the seedlings are planted, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are applied to culturing land, and after the fertilizers are applied, the land is turned over and leveled, wherein the applying quantity ranges from 150 kg per mu to 200 kg per mu, when planting is carried out, the planting distance ranges from 50 cm to 60 cm, and double-line culturing is carried out; 3, plants are adjusted; 4, additional fertilizing is carried out, wherein when fruits begin to be borne, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are additionally applied, and the additionally-applying quantity ranges from 100 kg per mu to 150 kg per mu; 5, harvesting is carried out. According to the method, sufficient glutathione synthesis precursors and sufficient selenium sources are provided for the cucumbers from the seedling culturing stage, the cucumbers can absorb nutrition from the land in the whole growing period and convert the nutrition into glutathione and organic selenium through photosynthesis, and the content of the glutathione and the content of the organic selenium in the cucumbers are high.