The invention relates to a portable human body load physiological and biomechanical monitoring device which is characterized by comprising a physiological fatigue state detection unit, a biomechanical signal detection signal and a data acquisition and analysis unit. By adopting the portable human body load physiological and biomechanical monitoring device, a field load walking test can be carried out, and human body physiological and biomechanical data in a real load walking state are obtained. In addition, the device detects and displays the tightness degree and a balance state of the shoulder, the check and a waistband of a knapsack in real time, ensures correct usage of the knapsack, prevents the knapsack from load fatigue caused by improper usage of the knapsack, eliminates interference factors, and accordingly can really and objectively monitor the influence of knapsack weight, the walking time, the knapsack design and other factors on the human body load fatigue. The portable human body load physiological and biomechanical monitoring device can be widely applied to the research fields of anthropometry, biomechanics, ergonomics and the like.