The invention relates to the technical field of biological detection, in particular to a portable multifunctional electrochemical biological detector which comprises an electrochemical biological detector body, a telescopic pull rod, a temperature sensor, a control processor and a battery, a pull box is arranged on one side of the electrochemical biological detector body, and the temperature sensor is fixedly connected to one side of the electrochemical biological detector body. The control processor and the battery are fixedly connected to one side in the pulling box, the telescopic pull rodis fixedly connected to one side outside the pulling box, and a mounting plate is fixedly arranged on the inner side of the pulling box. By arranging the heat dissipation box, the air inlet fan, the dispersion pipe, the temperature sensor, the control processor, the dustproof filter screen, the disassembly frame, the quick disassembly device, the pull box, the lifting frame, the shock absorption plate, the rotating wheel and the dustproof cover, the detector has the functions of convenience in carrying, intelligent heat dissipation, shock absorption, buffering, protection, dust prevention, water prevention, convenience in use and the like.