79results about How to "Rapid detection and identification" patented technology

The present invention relates to, in part, methods, reagents and apparatuses for the detection of agents. The present invention also relates, in part, to compositions including, but not limited to, flow cells, assay chambers, reagent reservoir delivery units and devices for holding an assay chamber. The present invention also provides various components and combinations of components for various detection apparatuses. The present invention also relates to a portable agent detection apparatus that can be used in the field or at a point of care and is not limited to specialized laboratories or limited to use by highly skilled users.

Disclosed are oligonucleotide amplification primers and detection probes specific for the amplification and detection of pathogenic organisms, including for example, specific Influenza A H1N1 viral isolates. Also disclosed is a biological organism identification kit including the disclosed nucleic acid probes and primers, as well as thermal cycling reagents that is both portable and durable, and may also be self-contained for remote, or in-field analysis and identification of particular influenza isolates from a variety of biological specimen types.

A biological organism identification product, and methods of using the same, that include a collection device to collect one or more sample organisms, a fixing and transporting composition present in an amount sufficient to kill the sample organism(s) associated with the collection device, an extraction member to extract a sufficient amount of genomic nucleic acid from the sample organism(s) to facilitate identification; and a polymerase chain reaction component into which the sufficient amount of genomic nucleic acid can be dissolved. The amplified genomic material is exposed to molecules that bind to predetermined genomic sequences, providing the identification feature of the product. The biological organism identification product may be portable, durable, and self-contained.

A method for detecting spectral characteristics of multi-band moving objects. The method includes: 1) dividing a full field of view into several subfields of view, and scanning and extracting suspected objects in each subfield one by one; 2) correlating interrelated suspected objects in adjacent subfields via coordinates to determine objects of interest that exist in the full field of view; 3) calculating the speeds of the objects of interest; 4) calculating average speed of all of the objects of interest and classifying the objects of interest according to their average speed; 5) compensating and rectifying the objective spectrum obtained from calculation; and 6) matching the compensated and rectified objective spectrum with a spectrum fingerprint database whereby realizing recognition of the multi-band moving objects.

Bacteriophage are combined with a test sample in an incubator, and the bacteriophage-exposed test sample is conjugated and applied to a sample pad in contact with a lateral flow strip to determine the presence or absence of a target bacterium. The conjugation may be performed in the sample pad or prior to application of the bacteriophage-exposed test sample to the pad. The incubator comprises a bacteriophage container and an incubation container separated by a valve. The test sample may be inserted into the incubation chamber using a swab or a rod with a piercing tip and a sample collection eye. The valve comprises a breakable stem. An antibiotic may be added to the test sample to determine the antibiotic resistance or susceptibility of the bacterium.

The invention relates to a car-mounted laser radar tunnel detection system, an autonomous positioning method based on the tunnel detection system and a tunnel hazard detection method. The detection system comprises a vehicle platform, a positioning module, a laser radar detection module and an information fusion and detection module, wherein the vehicle platform is used for providing a vehicle running environment and energy supply; the positioning module comprises an autonomous positioning module and a satellite positioning module; the satellite positioning module can position the vehicle platform when a satellite positioning signal is received, and the autonomous positioning module can position the position of a vehicle which cannot be positioned by a satellite in a tunnel; the laser radar detection module is used for detecting the surface of the tunnel by utilizing laser; the information fusion and detection module is used for analyzing, processing and fusing position information of the autonomous positioning module / satellite positioning module and the measurement information of the laser radar detection module. The autonomous positioning method is a positioning method suitable for the detection system. The tunnel hazard detection method is a detection method based on the tunnel detection system. The car-mounted laser radar tunnel detection system has the advantages of high detection efficiency, capability of carrying out the real-time monitoring and the like.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

A method for identifying interferers in a radio communication band, such as the 2.4 GHz ISM band, uses a priori information of the interferers such as channel structure, channel frequency and channel bandwidth. Measured RSSI can be used to detect and identify interferers. RSSI measurements can be made at multiple frequencies within a channel of the interferer. Randomization of RSSI measurements improves the likelihood of interferer detection and identification if such interferer is present. Metrics other than RSSI can be used.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

The invention discloses an orchard bactrocera dorsalis insect damage recognition system based on digital signal processing (DSP) and the internet of things. The orchard bactrocera dorsalis insect damage recognition system comprises an installation support, a novel insect trap fixed on the installation support, an industrial camera arranged above the novel insect trap and a casing fixed on the installation support. A photoelectric detection module is arranged in the novel insect trap; a DSP digital image processing module, a scanning tunneling microscope (STM) 32 functional circuit, a global system for mobile communications (GSM) / general packet radio service (GPRS) wireless communication module and a charging control module which are sequentially connected are arranged in the casing; a displayer and a touch screen are arranged on the surface of the casing; and the charging control module is further connected with a solar panel. According to the orchard bactrocera dorsalis insect damage recognition system, the DSP technology, the machine vision technology and the internet of things technology are combined, the machine vision technology is achieved on a DSP processor platform and applied to detection and recognition of orchard bactrocera dorsalis, and accordingly fast and accurate detection and recognition of orchard bactrocera dorsalis are achieved. Simultaneously, automatic real-time monitoring of orchard insect damage is achieved and cost of manpower and material resources in orchard insect damage monitoring is reduced.

The embodiment of the invention provides an image detection and recognition method and device, and the image detection and recognition method comprises the steps: inputting the aerial image data of anunmanned plane into a network model of a Faster-RCNN, and obtaining the accurate position of a target object outputted by the network model of the Faster-RCNN, wherein the network model of the Faster-RCNN is obtained by training the classification probability and the frame regression according to the detection classification probability and the detection frame regression. Image data collected byan unmanned aerial vehicle platform is combined based on an image recognition technology; a target detection algorithm in deep learning is applied and a learning model with a specific depth is trainedso that accurate positioning and identification of the target object can be realized; and the complex image preprocessing process can be reduced, and the detection efficiency is high, and the detection precision is high, and the practicality is high, and the adaptability and the reliability are great, and the target object can be rapidly detected and identified.

The invention relates to a botryosphaeria dothidea detection method, and in particular relates to a triplex real-time fluorescent polymerase chain reaction (PCR) detection method for botryosphaeria dothidea, belonging to the technical field of inspection and quarantine. According to the method disclosed by the invention, three groups of botryosphaeria dothidea specific primers and probes are designed and a reaction system is optimized, so that a triplex real-time fluorescent quantitative PCR detection method is established, and the detection method can be used for simultaneously detecting and identifying multiple pathogenic fungi and determining a composite invading pathogen. By applying nano magnetic microparticles to the extraction of fungal nucleic acid, the fungal nucleic acid extraction is simpler and more efficient in operation; and meanwhile, in combination with a real-time fluorescent detection technology, the detection method is applicable to the monitoring of disease occurrence in fields as well as the rapid and high-throughput detection and identification of pathogenic bacteria.

The invention provides a CAPs (C1eaved Amplified Polymorphic Sequences) molecular marker for identifying amaranthus tuberculatus based on an SNP (Single Nucleotide Polymorphism) site and application thereof. The molecular marker is located on ITS (Internal Transcribed Spacers) and 26S sequences of an amaranthaceous plant nuclear gene, and a nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1. Primer pair sequences for amplifying the molecular marker are as shown in SEQ ID NO.2 and SEQ ID NO.3. The specific molecular marker provided by the invention can realize rapid and accurate detection of the amaranthus tuberculatus and a reliable detection method is provided for rapid distinguishing and identification of alien invasive species.

The invention discloses a method for testing and identifying cepaea hortensis through polymerase chain reaction (PCR), and the method comprises the following steps that a specific primer is applied to identify the molecular features of cepaea hortensis; a forward primer of the PCR is CN-(P1):5'-ACCTCCTTCCTTTCTACT-3', and a reverse primer is CN-(P2):5'-GTCAACATCTATCCCAAC-3'. The total volume of a PCR system is 25mu.L, including 12.5mu.L of 2*TaqPCRMasterMix, respectively 0.5mu.L of the forward and reverse primers, 2mu.L of a deoxyribonucleic acid (DNA) template, and the rest is sterilizing ddH2O; and PCR procedures are as follows: predegeneration is carried out for 5 minutes at 5DEG C, 50 seconds at 95DEG C, 30 seconds at 52DEG C and 50 seconds at 72DEG C, and the process is repeated for 35 times; and extension is carried out for 10 minutes at 72DEG C, and the reaction is over. According to the method for testing and identifying cepaea hortensis through PCR, the cepaea hortensis can be quickly and accurately tested and identified, a novel technical means is provided for the testing and the identification of the dangerous and harmful creature cepaea hortensis, and important significance in preventing the cepaea hortensis from being spread and diffused is realized.

The invention relates to an illegal website identification system and method based on a critical path. A system framework is divided into four layers, namely a user layer, an application service layer, a technical support layer and a data storage layer. The user layer provides a main account of the system; the application service layer provides a main function module of the system; the technical support layer comprises a relevant tool used in the system development process and a core algorithm program; the data storage layer provides data used in the system. According to the detailed functional division, the function module of the illegal website identification system based on the critical path comprises data preprocessing, website similarity calculation, website clustering, illegal website critical path extraction and illegal website identification. The system develops a similarity calculation program based on the Path through taking URL self characteristics as research start points, can accurately calculate the similarity among websites and obtain the effective URL critical path based on the website similarity and a Fast Unfolding clustering algorithm, and finally can discover illegal websites in unknown websites through the URL critical path.

The invention provides a method of detecting and identifying cernuella virgata da costa through a fluorescent quantitation PCR. Molecular characteristics of cernuella virgata da costa can be identified by using a specific primer and a probe, an upstream primer of the fluorescent quantitation PCR is CVF:5'-CGTTTCGGGTGGGAGGGTTA-3', a downstream primer of the fluorescent quantitation PCR is CVR:5'-AAGTCGTCCGAGCTGTTCCG-3', the fluorescent probe is CVP:5'-FAM-AACCCAGGGCGCCTCCGCGG-BHQ-3', wherein a fluorescent reporting group which is marked by a 5' end is FAM, and a fluorescent quenching reporting group which is marked by a 3' end is BHQ. The method can detect and identify cernuella virgata da costa quickly and precisely, the method has the advantages of high specificity and sensitivity, short consuming time, and precise and convenient result determination, PCR post processing is not needed, and a false positive and cross pollution can be avoided effectively.

The invention discloses a method for detecting and identifying Cernuella virgata Da Costa by PCR. The method comprises identifying molecular characteristics of the Cernuella virgata Da Costa by specific primers, wherein the upstream primer of PCR reaction is HP- (P1): 5'-GTTATTGTTACTGCTCACGC -3', the downstream primer is HP- (P2): 5'-CTGCTAAGACAGGGAGAGAT-3'. The total volume of the PCR reaction system is 25 muL, wherein the volume of 2*TaqPCRMasterMix is 12.5 muL, the volume of the upstream and downstream primers are respectively 0.5 muL, the volume of DNA template is 3 muL and the rest volume is supplied with sterile ddH2O; PCR reaction program comprises carrying out a denaturation at 94 DEG C for 5 minutes at 94 DEG C for 30 seconds, at 46 DEG C for 30 seconds, at 72 DEG C for 1 minute, circulating for 25 times, extending at 72 DEG C for 10 minutes and completing the reaction. The Cernuella virgata Da Costa can quickly and accurately detected and identified by the method disclosed by the invention. The method provides a new technical means for the detection and identification of the Cernuella virgata Da Costa in the 'List of Quarantine Pests of the People's Republic of China of the Entry of Plants' and is of great significance for preventing the Cernuella virgata Da Costa from spreading and diffusing.

The invention provides a method for detecting and identifying theba pisana through fluorescent quantitative PCR (polymerase chain reaction). Specific primers and a probe are used for identifying the molecular features of the theba pisana; an upstream primer of the fluorescent quantitative PCR is TPF: 5'-GCTGGAAAGTGGAGGCCGTA-3'; a downstream primer is TPR: 5'-GCGGCGTCGGTTAAGAGAGA-3'; a fluorescence probe is TPP: 5'-FAM-AGCTGTTCCGGGCACCAAGACGTCACG-BHQ-3'; 5' end marked fluorescence reporter gene is FAM; 3' end marked fluorescence quenching gene is BHQ. The method has the advantages that the theba pisana can be fast and accurately detected and identified; the specificity is high; the sensitivity is high; the consumed time is short; the result judgment is accurate and convenient; the PCR post treatment is not needed; the false positive and the cross contamination can be effectively avoided.

This invention is a MP testing and identifying method, including distill DNA of samples to be tested, use marked MP general lead to expand the DNA of pending tested samples in PCR method, hybridize the PCR expanded products with the MP species-specific probe markedly located on the nylon film, wash the un-hybridized PCR expanded products on the nylon film, display the hybridizing results and etc, and meanwhile, this invention also provides the MP general lead, the MP species-specific probe and the reagent boxes of testing and identifying MP. The probe of this invention is delicate, and the testing and identifying are fast sensitive and the cost is low.

The invention discloses a method for detecting and identifying Helix aspersa MUller through PCR (polymerase chain reaction). The method comprises the step of identifying the molecular feature of the Helix aspersa MUller by using specific primers, wherein the upstream primer of PCR is HP-(P1): 5'-ACTCTTTGGTATTTGATGCG-3', and the downstream primer is HP-(P2): 5'-ACCTGCTAAAACGGGTAATG-3'. The method can be used for quickly and accurately detecting and identifying the Helix aspersa MUller. The method provides a new technical means for detection and identification of the Helix aspersa MUller in Imported Plant Quarantine Pest Directory of the People's Republic of China, and has significance for preventing the propagation and diffusion of the Helix aspersa MUller.

The embodiment of the invention discloses an embedded part detection method, device, equipment and system of a component production line, wherein the method comprises the steps of: obtaining the position information of an embedded part to be placed, an initial mould platform image that an embedded part is not placed, and a mould platform image that the embedded part is placed; according to the position information, extracting the initial projection contour line in the initial mould platform image, the laser contour line in the mould platform image and the image characteristics of the embeddedpart; according to a comparison result of the initial projection contour line, the laser contour line and the standard laser contour line, generating an embedded part position detection result, wherein the standard laser contour line is a laser contour line when the placement position of the embedded part to be placed on the mould platform accords with the standard requirement; and, according to acomparison result of the image characteristics of the embedded part and the image characteristics of the embedded part to be placed, generating an embedded part type detection result; thereby, rapidand accurate detection on the placement position and the type of the embedded part can be realized; the automation degree and the production efficiency of a prefabricated part are improved; and the manual labour workload is reduced.

Disclosed is a wireless power supply heating device, a heating method, a placing material and a preparation method thereof. The wireless power supply heating device is used for recognizing the placingmaterial placed in the working area and heating the recognized placing material. The wireless power supply heating device comprises a control module, a storage module connected to the control moduleand used for storing threshold value of worm current loss power which changes with the field frequency of the pacing material needs to be recognized, a coil used for generating a magnet field, a frequency scanning and power adjusting module used for inputting current to the coil, and a frequency response signal collecting module used for detecting parameters of the worm current loss power which changes with the field frequency of the pacing material. The control module controls the frequency scanning and power adjusting module to generate a magnetic field with stable frequency and heats the recognized placing material; in the condition of not adding any circuit at a heating end, effective recognition, safety and reliability of the placing material is achieved.

The present invention provides methods, devices and test kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing solid nutrient media. The invention includes mixing the one or more target microorganisms with one or more aptamers and / or one or more antibodies, each conjugated to a reporter compound and specific for a first site on the one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and / or one or more antibodies, each specific for a second site on the one or more target microorganisms or a site on the aptamer conjugate and / or antibody conjugate. A detection solution is added to the membrane, and detection and identification of the one or more target microorganisms is achieved in less than one hour.