64 results about "Nucleic acid cleavage" patented technology

The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.

The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

The present invention discloses a nucleic acid cleavage assay for members of the transposase / integrase superfamily. A method of using the assay to screen for modulators of the nucleic acid cleavage activity is also disclosed. The present invention further provides a method for screening for modulators of binding of a transposase / integrase to its corresponding recognition sequence. In addition, the present invention provides a method of identifying a modulator for a particular transposase / integrase such as HIV integrase based on modulators of other members of the transposase / integrase superfamily. Also disclosed are Tn5 transposase inhibitors and HIV integration inhibitors.

A method for extract nucleic acid includes such step as cracking nucleic acid sample, adding sample containing nucleic acid to cracking solution to form nucleic acid cracking solution, cracking nucleic acid sample to form nucleic acid cracking solution, cracking nucleic acid sample to obtain nucleic acid sample, cracking nucleic acid sample to obtain nucleic acid sample, cracking nucleic acid sample to obtain nucleic acid sample, cracking nucleic acid sample to obtain nucleic acid sample, cracking nucleic acid sample to obtain nucleic acid sample, cracking nucleic acid sample to obtain nucleicacid sample. 2, adsorb nucleic acid: adding that carry into the nucleic acid cleavage solution to obtain a carrier for adsorbing nucleic acid; 3, remove impurities: rin that carrier adsorbed with nucleic acid in a rinsing solution to remove impurities other than nucleic acid; 4, amplification reaction, wherein that rinse carrier adsorb with nucleic acid is directly added into a nucleic acid amplification system for amplification reaction. The carrier is cellulose filter paper; a simple, a method for extract nucleic acid based on cellulose filt paper is disclosed. Applicable to polymerase chain reaction amplification and recombinase polymerase isothermal amplification and other nucleic acid amplification technology, suitable for operating at room temperature, suitable for biological samples from various sources, can be loaded with nucleic acid cellulose filter paper directly into the nucleic acid amplification system for amplification reaction, the whole process is less time-consuming.

The invention provides a detection system for enriching low-abundance mononucleotide mutant genes based on a nuclease-coupled PCR principle. The invention particularly provides a method for increasingthe relative abundance of a target nucleic acid, which comprises the following steps: (a) providing a nucleic acid sample containing a target nucleic acid and a non-target nucleic acid, wherein the abundance of the target nucleic acid in the nucleic acid sample is F1a; (b) using the nucleic acid in the nucleic acid sample as a template, performing PCR and nucleic acid cleavage reaction in the amplification-cleavage reaction system to obtain amplification-cleavage reaction product; the target nucleic acid is amplified the abundance of the amplification-cleavage reaction product is F1b, and theratio of F1b / F1a is more than or equal to 10. The detection system has the advantages of non-invasion, easy operation, high speed and the like, the sensitivity can reach 0.01%, and the DNA quantity of the sample can be reduced to aM grade.

The present invention provides a method of detecting a genetic polymorphism in an individual or between individuals. The method comprises the following steps, (1) obtaining a sample containing nucleic acid from an individual; (2) contacting the sample, under conditions which permit primer-initiated nucleic acid amplification and nucleic acid cleavage, with (i) a primer suitable for initiating amplification, (ii) an indicator system which provides a signal proportional to the amount of amplification product, and (iii) a sequence specific nucleic acid cleavage agent; and (3) measuring the signal produced by the indicator system against time. Cleavage of the amplification product by the cleavage agent results in an inhibition of the rate of accumulation of amplification product comprising the sequence recognised by the cleavage agent relative to the rate of accumulation of amplification product not comprising the sequence recognised by the cleavage agent.

The present invention discloses a nucleic acid cleavage assay for members of the transposase / integrase superfamily. A method of using the assay to screen for modulators of the nucleic acid cleavage activity is also disclosed. The present invention further provides a method for screening for modulators of binding of a transposase / integrase to its corresponding recognition sequence. In addition, the present invention provides a method of identifying a modulator for a particular transposase / integrase such as HIV integrase based on modulators of other members of the transposase / integrase superfamily. Also disclosed are Tn5 transposase inhibitors and HIV integration inhibitors.