The invention relates to a premix for livestock breeding, made mainly from the following materials: 10-30 parts of composite multiple vitamin, 30-50 parts of anhydrous dextrose, 1-10 parts of potassium chloride, 1-10 parts of sodium chloride, 1-3 parts of magnesium chloride, 0.1-2 parts of L carnitine, 0.1-1 part of organic chromium, 0.1-2 parts of citric acid, 0.1-2 parts of medicated leaven, 4-10 parts of Rhizoma Atractylodis Macrocephalae extract, 5-10 parts of radix Bupleuri extract, 2-8 parts of Poria extract, 1-5 parts of Herba Andrographitis extract, 5-15 parts of fructo-oligosaccharide and 20-40 parts of auxiliary yellow. A preparation method includes: stirring the composite multiple vitamin, anhydrous dextrose, potassium chloride, sodium chloride, magnesium chloride, L carnitine, citric acid, Rhizoma Atractylodis Macrocephalae extract, Radix Bupleuri extract, Poria extract, Herba Andrographitis extract, fructo-oligosaccharide and auxiliary yellow, adding organic chromium and medicated leaven, and stirring. The premix enables body immunity of livestock be achieved, and post-immunity antibody generation is promoted.