The invention discloses a manufacturing method of an assembly type prefabricated superposed beam with steel structure connection joints. The manufacturing method comprises the following steps that first, main structures, located under the top face of a concrete part, of a superposed beam reinforcement cage and the steel structure connection joints are manufactured; second, the main structures of the steel structure connection joints are connected to the ends of the superposed beam reinforcement cage; third. A formwork is erected, then concrete is poured, and the concrete part of the prefabricated superposed beam is formed; fourth, the formwork is disassembled, and then the parts, protruding the top face of the concrete part, of the steel structure connection joints are welded on the top face of the main structures of the steel structure connection joints. The prefabricated superposed beam manufactured by the method enables the structure of beam column connection joints to be simple, and construction is convenient. According to the prefabricated superposed beam, under the loading effect, the steel structure connection joints can deform and absorb energy first, has good ductility under the earthquake load effect, and is suitable for being applied and popularized to a construction site.