45results about How to "High coincidence rate" patented technology

The invention discloses an area-of-interest detection method based on Kinect. The area-of-interest detection method is used for solving the technical problem that an existing area-of-interest detection method based on an improved saliency map model is low in accuracy. According to the technical scheme, a Kinect3D camera lens is used for obtaining a two-dimensional RGB image and depth information; on the basis, the RGB image is used for extracting various visual features and establishing a multi-scale visual feature map; then the feature map and a depth map are fused to generate the saliency map, and a winner-take-all strategy is used for generating a two-value saliency map; finally, dilation is performed on the two-value saliency map to detect a final area of interest. The 3D image, generated by the Kinect camera lens, in a RGB-D format can be used for detecting the area of interest, wherein the area of interest is consistent with the result of perception through the human eyes. Under the same condition, the matching rate of the area of interest is increased to 91.2% from 82.5% in the background technology, which means the matching rate is increased by 8.7%, and the area of interest is automatically detected by the utilization of the method.

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequence from SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.

The invention provides a CYP2D6 gene mutation detection liquid-phase chip which comprises ASPE (Allele Specific Primer Extension) primers aiming at CYP2D6 C2850T and CYP2D6Deletion mutational sites, three microballoons respectively enveloped with a specific anti-tag sequence and amplification primers aiming at the CYP2D6C2850T and the CYP2D6 Deletion mutational sites. The CYP2D6 gene mutation detection liquid-phase chip can simultaneously detect aiming at the CYP2D6 C2850T and the CYP2D Deletion mutational sites and has excellent signal to noise ratio. The coincidence ratio with a sequencing method of the CYP2D6 gene mutation detection liquid-phase chip reaches up to 100 percent, and the CYP2D6 gene mutation detection liquid-phase chip has higher specificity and precision compared with intra-class correlation products.

The invention discloses a PCR (Polymerase Chain Reaction) primer, a kit and a liquid chip for detecting an RET fusion gene. The liquid chip comprises a PCR amplification primer, an ASPE (Allele Specific Primer Extension) primer which consists of tag sequences and specific primers, and microspheres, wherein the specific primers comprise the following sequences: SEQ ID NO.23 for K15;R12, SEQ ID NO.24 for K16;R12, SEQ ID NO.25 for K22;R12, SEQ ID NO.26 for K23;R12, SEQ ID NO.27 for K24;R8, SEQ ID NO.28 for K24;R11 and / or SEQ ID NO.29 for C1;R12. The liquid chip disclosed by the invention has a quite good signal to noise ratio and can be used for implementing amplification of seven fusion subtypes through one step, and the specific primers have quite good specificity.

The invention discloses an amplification primer for detecting food-borne pathogenic microorganisms and a liquid chip kit. The kit comprises an amplification primer pair designed for microorganism genes to be detected; and a forward primer and a reverse primer of microorganisms to be detected comprise at least one pair of SEQ ID NO. 1 and SEQ ID NO. 2 for enterobacter sakazakii, SEQ ID NO. 3 and SEQ ID NO. 4 for monocyte listeria monocytogenes, SEQ ID NO. 5 and SEQ ID NO. 6 for escherichia coli O157, SEQ ID NO. 7 and SEQ ID NO. 8 for staphylococcus aureus, SEQ ID NO. 9 and SEQ ID NO. 10 for shigella, SEQ ID NO. 11 and SEQ ID NO. 12 for salmonella, and SEQ ID NO. 13 and SEQ ID NO. 14 for vibrio parahaemolyticus, and magnetic beads coated with Anti-tag sequences. The amplification primer designed by the invention has excellent specificity, and can accurately distinguish and specifically amplify gene segments corresponding to various target detection pathogenic microorganisms. In addition, the sensibility of the detecting kit designed by the invention is greatly improved. An experiment result shows that sensibility of detection of seven food-borne pathogenic microorganisms can reach 10 CFU / mL.

The invention discloses a PCR (Polymerase Chain Reaction) primer, a kit and a liquid phase chip for detecting an ALK (Anaplastic Lymphoma Kinase) fusion gene. The liquid phase chip comprises a PCR amplification primer, ASPE (Allele Specific Primer Extension) primers and microspheres, wherein the ASPE primers consist of tag sequences and specific primers, wherein the sequences of the specific primers are SEQ ID NO.12 for K15; DEL15A20, SEQ ID NO.13 for K17; A20, SEQ IDNO.14 for K9; A20, SEQ ID NO.15 for T6; A20 and / or SEQ ID NO.16 for T3; A20. The liquid phase chip disclosed by the invention has a very good signal noise ratio and can amplify five fusion subtypes by a single step, and the specific primers have very good specificity.

The invention discloses an ALK gene rearrangement detection probe, a kit and a method. The detection probe comprises a first group of probes for labeling fluorescent dye and targeted to the upstream side of a breaking point, and a second group of probes targeted to the downstream side of the breaking point, wherein the first group of the probes are selected from at least one of probes as shown in SEQ ID NO.41-SEQ ID NO.50; the second group of the probes are selected from at least one of probes as shown in SEQ ID NO.51-SEQ ID NO.60; and the fluorescent dyes labeled by the two groups of the probes are different in color. A FISH probe in the ALK gene rearrangement detection kit provided by the invention is free from a repeated sequence, and capable of avoiding a cross reaction and undergoes hybridization recognition with an ALK target detection fragment in a chromosome; and the probe has the advantages of high accuracy, good specificity and low false positive rate.

The present invention discloses an oil-gas microorganism gene exploration method. The method comprises the following steps: respectively collecting samples of surface shallow layers above a known oilwell, a gas well and a dry well in an exploration area, performing high-throughput sequencing after extracting DNA, establishing a microbial community composition pattern diagram in the exploration area according to a sequencing result, respectively screening out characteristic microorganisms in surface soil above the oil / gas well in the exploration area according to the pattern diagram, and designing primers according to attribute characteristics and carrying out a fluorescent quantitative PCR detection on the samples in the whole exploration area to detect the number of the characteristic microorganisms. A contour line of the characteristic microorganisms obtained by the oil-gas microorganism gene exploration method has a high coincidence rate with the known well, has a good coincidencerate with a trap line, and can carefully describe an oil area.

The invention discloses a carbonate rock surface fracture reservoir body characterization method. The method comprises the steps: calculating a root mean square amplitude property in a first depth range of the carbonate rock surface and calculating a relative wave impedance property in a second depth range; establishing a regression function according to the calculated two properties, converting one attribute into the other attribute according to the regression function, depicting a fracture reservoir body according to the values of the converted and unconverted attributes to obtain two piecesof fracture reservoir body data, and performing the coupling of the fracture reservoir body data to obtain a coupled data body of the carbonate rock surface fracture reservoir body. The method solvesthe problem of characterization loss caused by using the root mean square amplitude attribute to characterize the surface layer of a carbonate rock, improves the coincidence rate between a characterization result and actual drilling, and provides reliable basis for the potential tapping of the residual oil and improving the recovery of the fracture-cavity type reservoir.

The invention discloses BRAP and PSMA6 gene SNP detection specific primers and a liquid phase chip. The liquid phase chip comprises an ASPE primer which is composed of a tag sequence at a 5' terminal and specific primers mutated for a target gene at a 3' terminal, wherein the specific primers respectively comprise a SEQ ID NO.7 and a SEQ ID NO.8 for a BRAP gene A96G SNP locus, a SEQ ID NO.9 and a SEQ ID NO.10 for a BRAP gene A80G SNP locus, and / or a SEQ ID NO.11 and a SEQ ID NO.12 for a PSMA6 gene C157G SNP locus; microspheres coated by an anti-tag sequence; and amplification primers. The coincidence rate between detection results of the BRAP and PSMA6 gene SNP detection liquid phase chip provided by the invention and results of a sequencing method is up to 100%.

The invention discloses a liquid phase chip for detecting mutation of GSTP1 (glutathione S transferase pl) genes, mainly comprising an ASPE primer consisting of a tag sequence at 5' end and a specific primer at 3' end and aiming at a mutant site, micro-balloons and an amplification primer; wherein the specific primer is SEQ ID NO.11 and SEQ ID NO.12 aiming at the A313G mutant site, and SEQ ID NO.13 and SEQ ID NO.14 aiming at the C341T mutant site; the tag sequence is selected from the group of SEQ ID NO.1 to SEQ ID NO.6; and the micro-balloons different color codes are respectively covered with the special anti-tag sequences. The liquid phase chip for detecting mutation of GSTM1(glutathione S transferase mu), GSTT1 (glutathione S transferase theta) and GSTP1 genes in the invention has a great signal-noise ratio; no cross reaction exists between the designed probes and the anti-tag sequences basically; the tag-tag sequence, the selection of the anti-tag tag sequence and the combinationof the tag-tag sequence with the specific ASPE primer can avoid the cross reaction, and realize parallel detection of a plurality of mutant sites.

The invention discloses PCR (Polymerase Chain Reaction) primers, a kit and a liquid-phase chip for detecting an ROS1 fused gene. The liquid-phase chip comprises PCR amplification primers, an ASPE primer and a micro-ball, wherein the ASPE primer is composed of a tag sequence and specific primers; the sequences of the specific primers are as follows: a probe pair C6; SEQ ID NO.34 of R32 and the probe pair C6; SEQ ID NO.35 of R34 and a probe pair C5; SEQ ID NO.36 of the R34 and a probe pair S2; SEQ ID NO.37 of the R32 and a probe pair S4; SEQ ID NO.38 of the R32 and a probe pair S4; SEQ ID NO.39 of the R34 and a probe pair SL13; SEQ ID NO.40 of the R32 and a probe pair SL4; SEQ ID NO.41 of the R34 and a probe pair E10; SEQ ID NO.42 of the R34 and a probe pair L16; SEQ ID NO.43 of R35 and / or a probe pair T5; SEQ ID NO.44 of the R35. The liquid-phase chip has a very good signal-noise ratio and can finish the amplification of 11 fused subtypes in one step; the specific primer has very good specificity.

The invention provides a CYP2D6 gene mutation detection liquid-phase chip which comprises ASPE (Allele Specific Primer Extension) primers aiming at CYP2D6 C2850T and CYP2D6Deletion mutational sites, three microballoons respectively enveloped with a specific anti-tag sequence and amplification primers aiming at the CYP2D6C2850T and the CYP2D6 Deletion mutational sites. The CYP2D6 gene mutation detection liquid-phase chip can simultaneously detect aiming at the CYP2D6 C2850T and the CYP2D Deletion mutational sites and has excellent signal to noise ratio. The coincidence ratio with a sequencing method of the CYP2D6 gene mutation detection liquid-phase chip reaches up to 100 percent, and the CYP2D6 gene mutation detection liquid-phase chip has higher specificity and precision compared with intra-class correlation products.

The invention provides a method and a chip for detecting multidrug-resistant mycobacterium tuberculosis. The method and the chip disclosed by the invention relate to detection of katG315 mutant I and / or II or rpoB526 mutant I and / or II, rpoB531 mutant I and / or II, and inhA-15 mutant. The invention also provides a primer for detecting the mutants.

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of MTHFR and FGF5 genes. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 9 and SEQ ID NO. 10 specific for a G121A SNP site, SEQ ID NO. 11 and SEQ ID NO. 12 specific for an A105G SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a G111A SNP site, and / or SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T196A SNP site of the FGF5 gene. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the MTHFR and FGF5 gene and the detection result of a sequencing method is up to 100%.

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequencefrom SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.

The invention discloses a C6ORF97 gene mutation detection liquid chip, and specific primers. The C6ORF97 gene mutation detection liquid chip mainly comprises: ASPE primers, wherein each ASPE primer is composed of 5'-terminal tag sequence, and 3'-terminal specific primer sequence targeting target gene mutation sites, and the specific primer sequence comprises SEQ ID No.9 and SEQ ID No.10 targeting C1810T site, SEQ ID No.11 and SEQ ID No.12 targeting T91235C site, SEQ ID No.13 and SEQ ID No.14 targeting A68C site, and / or SEQ ID No.15 and SEQ ID No.16 targeting A123001G site; microballoons coated with anti-tag sequence; and amplification primers. Self-agreement ratio of detection results of the liquid chip with detection results of sequencing is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is realized.

The invention discloses ABCB2 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of tag sequence and specific primer sequences aiming at mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.13 and SEQ ID NO.14 aiming at G142A site; SEQ ID NO.15 and SEQ ID NO.16 aiming at G88A site; SEQ ID NO.17 and SEQ ID NO.18 aiming at G154A site; SEQ ID NO.19 and SEQ ID NO.20 aiming at C86T site; SEQ ID NO.21 and SEQ ID NO.22 aiming at C150T site; and / or SEQ ID NO.23 and SEQ ID NO.24 aiming at A92T site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.

The invention discloses BRAP and PSMA6 gene SNP detection specific primers and a liquid phase chip. The liquid phase chip comprises an ASPE primer which is composed of a tag sequence at a 5' terminal and specific primers mutated for a target gene at a 3' terminal, wherein the specific primers respectively comprise a SEQ ID NO.7 and a SEQ ID NO.8 for a BRAP gene A96G SNP locus, a SEQ ID NO.9 and a SEQ ID NO.10 for a BRAP gene A80G SNP locus, and / or a SEQ ID NO.11 and a SEQ ID NO.12 for a PSMA6 gene C157G SNP locus; microspheres coated by an anti-tag sequence; and amplification primers. The coincidence rate between detection results of the BRAP and PSMA6 gene SNP detection liquid phase chip provided by the invention and results of a sequencing method is up to 100%.

The invention discloses a carbonate rock surface fracture reservoir body characterization method. The method comprises the steps: calculating a root mean square amplitude property in a first depth range of the carbonate rock surface and calculating a relative wave impedance property in a second depth range; establishing a regression function according to the calculated two properties, converting one attribute into the other attribute according to the regression function, depicting a fracture reservoir body according to the values of the converted and unconverted attributes to obtain two piecesof fracture reservoir body data, and performing the coupling of the fracture reservoir body data to obtain a coupled data body of the carbonate rock surface fracture reservoir body. The method solvesthe problem of characterization loss caused by using the root mean square amplitude attribute to characterize the surface layer of a carbonate rock, improves the coincidence rate between a characterization result and actual drilling, and provides reliable basis for the potential tapping of the residual oil and improving the recovery of the fracture-cavity type reservoir.

The invention provides a method and a chip for detecting multidrug-resistant mycobacterium tuberculosis. The method and chip of the present invention relate to the detection of katG315 mutant type I and / or II, rpoB526 mutant type I and / or II, rpoB531 mutant type I and / or II, and inhA-15 mutant type. The present invention also provides primers for detecting the above-mentioned mutants.

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of MTHFR and FGF5 genes. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 9 and SEQ ID NO. 10 specific for a G121A SNP site, SEQ ID NO. 11 and SEQ ID NO. 12 specific for an A105G SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a G111A SNP site, and / or SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T196A SNP site of the FGF5 gene. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the MTHFR and FGF5 gene and the detection result of a sequencing method is up to 100%.

The invention relates to a DNA tag and a pair of PCR primers connected to the DNA tag at the 5' end. The invention also provides a method and a kit for constructing a sequencing library consisting of the DNA tag and the pair of PCR primers. The DNA tag provided by the present invention and the amplification primer form a detection product that achieves optimization and balance between specificity, sensitivity and repeatability, and can detect 1-20 samples from different sources at a time, and can accurately distinguish samples from various sources. For the base sequence, the coincidence rate between the high-throughput sequencing described in the present invention and the sequencing method is as high as 100%.

The present invention discloses a detection liquid phase chip and specific primers for chromosome Xp11.22 segment polymorphism. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises A65G site-targeted SEQ ID NO.7, A65G site-targeted SEQ ID NO.8, G121A site-targeted SEQ ID NO.9, G121A site-targeted SEQ ID NO.10, and / or C162T site-targeted SEQ ID NO.11 and C162T site-targeted SEQ ID NO.12; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.

The invention discloses a liquid phase chip and a specific primer for detecting RAD51L1 gene mutation. The liquid phase chip mainly comprises: ASPE primers, microspheres coated by different anti-tag sequences, and an amplification primer, wherein each ASPE primer is composed of a tag sequence at a 5' end and specific primer sequences at a 3' end and aiming at target gene mutation sites, and the specific primer sequences are as follows: SEQ ID NO.9 and SEQ ID NO.10 aiming at a C167T site, SEQ ID NO.11 and SEQ ID NO.12 aiming at an A76T site, SEQ ID NO.13 and SEQ ID NO.14 aiming at a T187G site, and / or SEQ ID NO.15 and SEQ ID NO.16 aiming at a T99G site. The coincidence rate of the detection result of the detection liquid phase chip disclosed by the invention with that of a sequencing method reaches as high as 100%, and parallel detection of a wild type and a mutant of a plurality of mutation sites is achieved.

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of a chromosome 4q25 section. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 11 and SEQ ID NO. 12 specific for a C172T SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a C133A SNP site, SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T139G SNP site, SEQ ID NO. 17 and SEQ ID NO. 18 specific for a T80A SNP site, and / or SEQ ID NO. 19 and SEQ ID NO. 20 specific for a T105C SNP site. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the chromosome 4q25 section and the detection result of a sequencing method is up to 100%, not only is the condition of single site mutation detected, but also the polymorphism conditions of multiple mutant sites can be simultaneously detected in parallel, and the detection effects are consistent.

The invention provides a liquid-phase chip for chromosome 13q22 region SNP detection. The liquid-phase chip mainly comprises: ASPE primers composed of tag sequences of 5' end and sequences of specific primers for SNP sites of target genes of 3' end, wherein the sequences of the specific primers are SEQ ID No.1 and SEQ ID No.2 for A94G site, and / or SEQ ID No.3 and SEQ ID No.4 for G288A site; microballoons coated by anti-tag sequences; and amplimers. The liquid-phase chip for chromosome 13q22 region SNP detection and a sequencing method reach 100% agreement in detection results, and parallel detection of wide type and SNP type of a plurality of SNP sites can be realized.

The invention discloses a Japanese full-keyboard input method and device and electronic equipment, and the Japanese full-keyboard input method comprises the following steps: in a Japanese full-keyboard input mode, receiving key input information, where the key input information comprises one or more pieces of continuous Roman tone information corresponding to keys; converting the key input information into peaceful and kana information according to a peaceful and kana conversion table; obtaining Japanese character strings corresponding to the key input information according to the peacename and kana information; and outputting the Japanese character string. The concept of the invention lies in that all Roman tones input through keys are converted into corresponding peas and kana, and all converted peas and kana are decoded as a whole to obtain an accurate Japanese character string, so that the coincidence rate of an output result and the expectation of a user is improved, and the input efficiency is further improved.