The invention relates to a preparation method of immune colloidal gold particles capable of being used for rapid diagnosis, which comprises the following steps: sequentially adding chloroauric acid, hexadecyl triethylammonium bromide and ultrapure water under the condition that the temperature of a water bath is 35 DEG C, uniformly stirring and mixing, further adding NaBH4 after 4-6min, quickly stirring for 2-3min, and then slowly stirring; continually carrying out reaction in the water bath for 2-12h, leading the NaBH4 in solution to volatilize completely, and completing the synthesis of gold particles of 3-4nm; carrying out synthesis by taking the synthesized gold particles of 3-4nm as gold seeds when synthesizing the colloidal gold particles with the particle size of below 30nm: diluting the solution of the gold seeds according to the proportion of 1:200, sequentially adding CTAB and the chloroauric acid, fixing the volume to be slightly less than the target volume, then adding ascorbic acid, and finally adding the gold seeds, wherein the different adding quantities of the chloroauric acid can synthesize the gold particles in different size ranges below 30nm; and carrying out the synthesis by taking the synthesized 30nm gold particles as the gold seeds when synthesizing the colloidal gold particles with the particle size of above 30nm: diluting the solution of the gold seeds according to the proportion of 1:200, sequentially adding hydroxylamine hydrochloride and the chloroauric acid, and uniformly stirring and mixing; adding a small amount of the chloroauric acid every 1h after carrying out the reaction for 5-8h, and adding the chloroauric acid for three times; and continuing the reaction for 2h, and obtaining colloidal gold solution.