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Preparation method of immune colloidal gold particles capable of being used for rapid diagnosis

A technology for colloidal gold particles and rapid diagnosis, which is applied in the field of preparation of immune colloidal gold particles, can solve the problems of difficult control of the size of colloidal gold particles, uneven particle size distribution, and affecting applications, and achieve accurate and reliable detection results, uniform size, Effects in simple steps

Active Publication Date: 2013-09-18
HANGZHOU KITGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing studies have shown that when the particle size of colloidal gold particles is about 10-40nm, the specificity and sensitivity of the method are better. However, the commonly used process for the preparation of colloidal gold particles is the trisodium citrate reduction method. The obtained colloidal gold particles The size of the particles in this particle size range is not easy to control, and the particle size distribution is not uniform, which seriously affects its application in rapid diagnosis

Method used

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  • Preparation method of immune colloidal gold particles capable of being used for rapid diagnosis
  • Preparation method of immune colloidal gold particles capable of being used for rapid diagnosis
  • Preparation method of immune colloidal gold particles capable of being used for rapid diagnosis

Examples

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Embodiment 1

[0018] Embodiment 1: A method for preparing immune colloidal gold particles that can be used for rapid diagnosis mainly includes the following steps: (1) Add chloroauric acid (HAuCl 4 ), cetyltriethylammonium bromide (CTAB) and ultrapure water, stir and mix well, after 4-6min, add NaBH 4 , stirring rapidly for 2 to 3 minutes, and then slowly stirring; (2) continue the water bath for 2 to 12 hours to make the NaBH in the solution 4 The volatilization is complete, that is, the synthesis of 3-4nm gold particles is completed; (3) when synthesizing colloidal gold particles with a particle size below 30nm, use the synthesized 3-4nm gold particles as gold species for synthesis: dilute gold at a ratio of 1:200 Seed solution, add CTAB and chloroauric acid successively, set the volume to slightly less than the target volume, then add ascorbic acid, and finally add gold seeds, the difference in the amount of chloroauric acid added in this step can synthesize gold particles of different s...

example 1

[0019] Example 1: Synthesis of colloidal gold seed particles: first, prepare the mixed solution of chloroauric acid and CTAB so that the concentration of chloroauric acid in the solution is 0.25mmol / L, and the concentration of CTAB is 75mmol / L; 0.6mmol / L NaBH4, stir and mix well to obtain colloidal gold seed particles with an average particle size of 3~4nm, which can be used to synthesize particles with other particle sizes.

example 2

[0020] Example 2: Synthesis of colloidal gold particles with a particle size of 10nm: first, dilute the gold seed solution at a ratio of 1:200; then, add CTAB, chloroauric acid, ascorbic acid and gold that accounts for 1 / 1000 of the total volume of the final synthesized colloidal gold solution in sequence A mixed solution of the diluent, so that the concentration of CTAB in the solution is 16mmol / L, the concentration of auric acid chloride is 1.11mmol / L, and the concentration of ascorbic acid is 6mmol / L; finally, add a little auric acid chloride every 1h, add in three times, The concentration of chloroauric acid in the final solution is increased by 0.25mmol / L, and colloidal gold particles with an average particle diameter of 10nm can be reacted.

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Abstract

The invention relates to a preparation method of immune colloidal gold particles capable of being used for rapid diagnosis, which comprises the following steps: sequentially adding chloroauric acid, hexadecyl triethylammonium bromide and ultrapure water under the condition that the temperature of a water bath is 35 DEG C, uniformly stirring and mixing, further adding NaBH4 after 4-6min, quickly stirring for 2-3min, and then slowly stirring; continually carrying out reaction in the water bath for 2-12h, leading the NaBH4 in solution to volatilize completely, and completing the synthesis of gold particles of 3-4nm; carrying out synthesis by taking the synthesized gold particles of 3-4nm as gold seeds when synthesizing the colloidal gold particles with the particle size of below 30nm: diluting the solution of the gold seeds according to the proportion of 1:200, sequentially adding CTAB and the chloroauric acid, fixing the volume to be slightly less than the target volume, then adding ascorbic acid, and finally adding the gold seeds, wherein the different adding quantities of the chloroauric acid can synthesize the gold particles in different size ranges below 30nm; and carrying out the synthesis by taking the synthesized 30nm gold particles as the gold seeds when synthesizing the colloidal gold particles with the particle size of above 30nm: diluting the solution of the gold seeds according to the proportion of 1:200, sequentially adding hydroxylamine hydrochloride and the chloroauric acid, and uniformly stirring and mixing; adding a small amount of the chloroauric acid every 1h after carrying out the reaction for 5-8h, and adding the chloroauric acid for three times; and continuing the reaction for 2h, and obtaining colloidal gold solution.

Description

technical field [0001] The invention relates to a method for preparing immune colloidal gold particles that can be used for rapid diagnosis, which is greatly improved in terms of particle shape control, particle size control, and particle size uniformity, etc., and belongs to the preparation method of immune colloidal gold particles. technology field. technical background [0002] The origin of colloidal gold technology can be traced back to 1971. Faulk et al. applied electron microscope immunocolloidal gold staining (IGS) to observe Salmonella, and first introduced colloidal gold into immunochemistry. Since then, immunocolloidal gold technology has been widely used as a new immunological method. It is applied to electron microscope level research, light microcytochemistry, immunoprecipitation and protein staining, and has been introduced into the field of immunodiagnosis, especially in the field of rapid diagnosis of medical testing, showing great development prospects. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24
Inventor 李国强王锐康俊奚建宏
Owner HANGZHOU KITGEN BIOTECH
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