69results about How to "Do not use or reduce dosage" patented technology

The invention relates to a biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof, belonging to agriculture intensification production technologies. Trichoderma harzianum SQR-T037 having obvious biological preventing and controlling effect on cucumber and watermelon blight is separated, and the microbe organic fertilizer is prepared from the biological preventing and controlling strain and organic compost; the fertilizer contains more than 1*108 bacterial colony forming units per gram of the strain, the full nitrogen content is 3-5 percent (more than 90 percent is organic nitrogen), the total NPK nutrient content is 6-10 percent, and the organic matter content is 30-35 percent. An experiment indicates that the microbe organic fertilizer leads the biological preventing and controlling strain to be rapidly bred after being applied to the soil and forms a preponderant population in the soil, and the biological preventing and controlling rate of the blight in the soil having serious cucumber and watermelon blight reaches more than 85 percent. By applying the microbe organic fertilizer to the soil without forming continuous cropping obstruction for a long time, the preventing and controlling rate on the cucumber and watermelon blight reaches more than 80 percent.

The invention relates to a bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease, belonging to the technical field of plant protection. The bacterial strain AT31 is shaking cultured in PDA culture solution at 37 DEG C and 180rpm for 20-24 hours, then is subject to centrifugation at 6000rpm for 10min, a finished bactericide product thereof has a total viable bacteria concentration of 1*10<9>-1*10<10>cfu / ml, can be used by pouring at roots when transplanting vegetable seedlings after being diluted by 100 times, or be manufactured into microbial fertilizer to be used a week before the vegetable seedlings are transplanted, and has a prevention effect of 58-82% for root knot nematode disease of tomato, loofah, cucumber and other vegetables. The bactericide is diluted by 100 times to be used in spray treatment on rice stem base, has a prevention effect of 64.7% for rice sheath blight disease, and can increase the yield by 22.33%. And the bactericide can also cure vegetable bacterial wilt and epidemic diseases, and can improve soil hardening phenomenon.

The invention relates to a preparation method of ethylene and propylene, in particular to a method for preparing light olefins in which ethylene and propylene are the primary products and methanol, carbon 4 and the hydrocarbons above carbon 4 are taken as raw material. In the process cracking ethylene and / or dimethyl ether to prepare the light olefins such as ethylene and propylene, part of from carbon 4 to carbon 18 alkane and olefin are added as feedings, and the scission reaction of hydrocarbon which absorbs heat is used for reducing the lot quantity of heat of reaction released from the cracking process of ethylene and / or dimethyl ether, thereby inhibiting the heat effect of the MTO reaction, raising the stability of the reaction system, allowing the operation easier, prolonging the service of catalytic agent, saving energy and reducing cost.

The invention relates to an antagonistic bacterium for preventing and killing off tobacco black shank and a microbial organic fertilizer thereof, belonging to a production technology of intensive agriculture. An antagonistic bacterium (paenibacillus polymyxa C-5) having obvious antagonistic effect on the tobacco black shank pathogenetic bacteria is separated and utilized to prepare into the microbial organic fertilizer with organic compost, wherein the microbial organic fertilizer respectively contains more than 1*108 C-5 / g, the total-N content is 4-5 percent (more than 90 percent is organic nitrogen), the total nutrient content of nitrogen, phosphorus and potassium is 6-10 percent, and the content of organic matters is 30-35 percent. Proved by experiments, the microbial organic fertilizer enables the antagonistic bacterium to quickly breed and form a preponderant group in soil after being applied to the soil. The microbial organic fertilizer can greatly prevent the continuous cropping tobacco black shank if being applied to soil without continuous cropping obstacles for a long time.

The invention relates to antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and a microbial organic fertilizer thereof, belonging to a technology of agricultural intensification production. In the invention, two antagonistic bacteria (bacillus subtilis NJHJ-5 and bacillus subtilis NJDF-14) having obvious antagonistic actions on pathogenic bacteria of the cotton greensickness are separated, and the microbial organic fertilizer is prepared by utilizing the antagonistic bacteria and organic composts, wherein the microbial organic fertilizer respectively contains more than 1*10<9> / g of the NJHJ-5 and the NJDF-14, the total-nitrogen content is 4-5 percent (more than 90 percent of the content is organic nitrogen), total nitrogen-phosphorus-potassiumnutrients are 6-10 percent, and the content of organic matters is 30-35 percent. Proved by experiments, after being fertilized into soil, the microbial organic fertilizer enables the antagonistic bacteria to quickly reproduce and form a dominant group in the soil. After the microbial organic fertilizer is continuously fertilized for three seasons, the prevention and cure rate of the cotton greensickness in the soil the original morbidity of which is higher than 60 percent can reach 100 percent.

The invention relates to a liquid composite biological multi-control fertilizer. The invention is characterized in that the liquid composite biological multi-control fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of Streptomyces hygroscopicus solution, 1-3 parts of Streptomyces cinnamofuscus solution, 2-4 parts of Bacillus amyloliquefaciens solution, 2-4 parts of bacillus sphaericus solution, 7-15 parts of Bacillus mucilaginosus solution, 1-3 parts of Azotobacter chroococcum solution, 1-3 parts of Streptomyces aureochrogmoenes, 8-10 parts of Trichoderma viride fermentative degradation solution, 8-10 parts of matrix and 45-65 parts of sterilized water. The invention prepares a crop-absorbing carbohydrate and organic matter Trichoderma viride fermentative degradation solution, and provides high-efficiency beneficial microbial florae with phosphate solubilizing, potassium solubilizing, nitrogen fixation and disease resistance effects. The fertilizer can simultaneously perform the three functions of biological pesticide, biofertilizer and biological nutrients, and has the advantages of no toxicity or harm and no environment pollution. Compared with other fertilizers with the same nutrients, the crop yield is increased by 20%.

The invention relates to antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt, which belongs to intensive agricultural production technology. According to the invention, a microbial organic fertilizer is produced from two antagonistic bacteria (brevibacillus brevis NJL-25 and bacillus cereus NJL-14) which have a remarkable antagonistic function on pathogenic bacteria of the tobacco bacterial wilt and are obtained by separation, and organic fertilizers, wherein 1 gram of the microbial organic fertilizer contains more than 1*10<10> NJL-25 and 1*10<10> NJL-14, and has the total nitrogen content of 4 to 5 percent (organic nitrogen content is over 90 percent), the total nitrogen-phosphorus-potassium content of 6 to 10 percent and the content of organic matters of 30 to 35 percent. Tests prove that the microbial organic fertilizer, after being applied to soil, can fast multiply the antagonistic bacteria to form a dominant group in the soil, achieve the biological prevention and control rate of over 97.6 percent on the bacterial wilt in the soil with the tobacco bacterial wilt, and prevent the occurrence of the continuous-cropping tobacco bacterial wilt to a great extent after being applied for long to the soil without continuous cropping obstacles.

The invention relates to a method for producing a microbial organic fertilizer by utilizing poultry feathers and a product thereof, and belongs to an agricultural intensive production technology. According to the method disclosed by the invention, the puffed feathers are taken as amino acid source carriers, mature pig manure compost and microbes of rapeseed meal are mixed for decomposing a mixture, and solid fermentation is performed for preparing the microbial organic fertilizer. The fertilizer contains more than 1 multiplied by 10<8> / g of functional bacterial, 2-4% of total nitrogen (organic nitrogen accounts for more than 90%), 3-5% of total nitrogen, phosphorus and potassium and 25-35% of organic matters. Test results show that the waste feathers are puffed and then mixed with the pig manure compost, secondary fermentation is performed for producing the biological organic fertilizer, and the total effects are better.

The invention relates to an antagonistic bacterium for controlling the bacterial wilt of capsicum and a microorganism organic fertilizer thereof, belonging to an agricultural intensified production technology. An antagonistic bacterium (bacillus subtilis a45) which plays an obvious antagonism role in pathogenic bacteria of the bacterial wilt of the capsicum is obtained by separation, and the disease-resistant microorganism organic fertilizer is prepared from the antagonistic bacterium and organic compost; and the fertilizer contains more than 2.0*109 a45 per gram, the total nitrogen content is 33.1g kg<-1>, the total phosphorus content is 13.3g kg<-1> and the total potassium content is 21.3g kg<-1>. Tests prove that the antagonistic bacterium can be rapidly bred after the microorganism organic fertilizer is applied to soil and forms a preponderant population in soil; and the preventing and treating rate on the bacterial wilt of the capsicum is as high as 70 percent.

The invention relates to growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and a microorganism organic fertilizer produced from growth-promoting bacteria, belonging to the technology of intensive agriculture production. A growth-promoting bacterial strain NJPRHSDAQ-1 is obtained through separation, and is inoculated into a microbial decomposition mixture of cured pig manure compost and rapeseed meal for solid fermentation so as to prepare the microorganism organic fertilizer, the fertilizer comprises more than 1*10<8>cfu / g of growth-promoting bacteria and 4-5% (above 90% of organonitrogen) of total nitrogen, the total nitrogen phosphorus and potassium nutrient is 6-10%, and the organic content is more than or equal to 45%. A pot experiment result proves that the rate for preventing and controlling the ginger soil from having the wilt achieves above 75%, and if being applied to soil in which the ginger is planted, microorganism organic fertilizer can prevent and control ginger soil-borne wilt.

The invention discloses an organic microbial fertilizer for lettuces and a preparation method thereof. The organic microbial fertilizer for lettuces is prepared from the following components in parts by weight: 4-7 parts of streptomyces aureus, 0.6-0.9 part of bacillus thuringiensis, 3-5 parts of enzyme bacteria, 900-1100 parts of waste liquid precipitates, 240-380 parts of an inorganic fertilizer, 45-55 parts of trace elements, 25-32 parts of an adhesive, 0.5-0.7 part of a stabilizer and 0.1-0.3 part of an effect-stabilizer assistant. The organic microbial fertilizer prepared by the method is capable of increasing the yield of the lettuce, the content of vitamin C and the content of an iron element; and the content of nitrate is reduced, so that the quality of the lettuce is enhanced greatly. Compared with other fertilizers containing the same nutrients, the organic microbial fertilizer is capable of decreasing or lowering crop disease-pest damages which affect the crop quality. The using quantity of a chemical fertilizer is not decreased, and the organic microbial fertilizer is beneficial to environmental protection.

The invention discloses a bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof. The invention provides a paenibacillus polymyxa strain G1 the preservation No. of which is CGMCC No. 6762. The invention also provides a method for preparing a G1 bacteria agent, which comprises the following steps: fermenting the paenibacillus polymyxa strain G1, and collecting fermented products so as to obtain the G1 bacteria agent. Experiments prove that aiming at the current situation that no bio-control agent which achieves a good effect on preventing pepper phytophthora blight is provided at present; and the invention provides a single bacterium for preventing pepper phytophthora blights and a preparation thereof, which are specially applied to the prevention and control of pepper phytophthora blights. The bacteria agent has a good prevention and control effect on important soil-borne diseases, and the control effect on greenhouse pepper phytophthora blights reaches 71.18%.

The invention provides a biologic bacterial strain XY21 for preventing and treating bacterial wilt in greenhouse vegetables, and pertains to the technical field for preventing and treating diseases in crops. The XY21 is verified to be Serratia sp., the culture preservation no. is CGMCC NO.2059. The XY21 bacterial strain expresses good colonization surrounding the roots of tomatoes. After soaking the culture for 14 days, the colonized volume of the bacterial strain surrounding a root of tomato tree is 5.05 is multiplied by 10<7>cfu / g soil. If the wet bacterial strain is mixed with a lab thallus-preservation solution in a proportion of 1:40 (g / ml), the bacterial strain can be stored 2-3 years under room temperature. Tests show that, by application XY21 bacterial strain in fields, the prevention effect is 64.84% and 79.80% respectively, the outputs of the diseased fields increased respectively 31.8% and 45.8%. The invention is of high prospective to be developed into commercial live-vaccine biologic preventing preparations.

The invention belongs to the technical field of crop disease prevention and treatment and discloses a bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof. The bio-control bacteria strain 1JN2 is named as Bacillus axarquiensis in classification, and is filed at General Microbiological Center of China General Microbiological Culture Collection Committee with CGMCC No.5731 on January 16, 2012. The bio-control bacteria strain 1JN2 is cultured in LB (luria-bertani) broth at the temperature of 28 DEG C by vibration of 180rpm for 12-16 hours, centrifuged by 6000rpm for 10 minutes, diluted by sterilization water to prepare bactericide, and total concentration of viable bacteria in the finished bactericide is 1X10<9>-1X10<10>CFU / ml. The experiment results in a greenhouse indicates that the bio-control bactericide with the 1JN2 has evident growth acceleration effect to watermelons, biomass live weight is increased to 55.92% and evident prevention effect to disease of fruit blotches of the watermelons, disease incidence can be reduced by 50.13%.

The invention provides a biological control compound microbial inoculum AR156 mixture for controlling vegetable soil-borne disease, belonging to the technical field of plant disease biological control. The microbial inoculum consists of three biological control bacteria of AR156(Bacillus sp.), XY21(Serratia sp.) and SM21 (Bacillus sp.). The three bacteria go through shaking culture respectively in PDA culture fluid under 37 DEG C and 180rpm for 20 to 24 hours, taking 4000g to be centrifugated for 10min, wetting bacteria and thallus preservative fluid are taken and made into microbial inoculum according to 1:40 (g / ml), and then evenly mixed according to the ratio of 1: 1: 1, the total concentration of viable bacteria in finished products is that 1 is multiplied by 10<9> to 1 is multiplied by 10<12>cfu / ml. The prevention effects on a potato family crop granuville wilt greenhouse and fields are respectively more than 90 percent and 70 percent, the prevention effect on potato family crop plagues is more than 65 percent, the prevention effect on vegetable root knot nematode diseases is 60 to 85 percent, the production can be increased by 50 to 200 percent, meanwhile the microbial inoculum can improve the phenomenon of soil hardening.

A biological control bacteria formulation for preventing green house vegetable disease comprises Bacillus circulans, Bacillus pumilus, Bacillus cereus, Bacillus Subtilis, Serratia marcescens, Pseudomonas fluorescens. The preparation method is : mixing the above-mentioned bacteria according to 1:0.4-0.5:0.2-0.3:0.2-0.3:0.2-0.3:0.2-0.3, 200rpm shaking 36 hours in PDA culture liquid in 33 DEG C, 7000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g / ml), the alive bacteria concentration being 1x10<9>-1x10<14>cfu / ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable disease can reach 75%, yield promoting effect is more than 45%.

The invention discloses a biocontrol strain HTC for preventing pepper phytophthora blight and application thereof, and provides a Chaetomium aureum strain HTC. The preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 6173. The invention further provides a method for preparing an HTC biocontrol agent for preventing the pepper phytophthora blight. The method comprises steps as follows: the Chaetomium aureum strain HTC is fermented, so that a fermentation product is obtained, namely, the biocontrol agent is obtained. The experiment of the invention proves that in a greenhouse, the strain HTC biocontrol agent has a preventive effect on the pepper phytophthora blight to a certain extent, the effect can reach 72.19%, the strain height is increased by 12.27% and the live weight is increased by 32.28% in a seedling stage; and the live weight is increased by 31.11% and the yield of a pepper fruit is increased by 21.81% in reaping time.

The invention discloses a biocontrol strain BS71 and an application thereof to preventing crops from being polluted by aflatoxin. The biocontrol strain BS71 is bacillus subtilis BS71, with collection number of CGMCC No.8191. The application method comprises the steps of preparing a biocontrol agent of the biocontrol strain BS71 and applying the biocontrol agent to the crops. The biocontrol strain has strong antagonistic action on aspergillus flavus and can be used for preventing pollution caused by aflatoxin. The invention provides a biocontrol method for pollution caused by aflatoxin. As chemical pesticides are not used, the biocontrol strain has the advantages of no toxicity, low possibility of having resistance to drugs, environmental and biological safety and the like, and has extensive application prospects.

The invention belongs to the technical field of biological material preparation and provides a bacillus subtilis wdcjxq2266, a biocontrol microbial agent produced from the strain and application of the strain and the biocontrol microbial agent. The bacillus subtilis wdcjxq2266 is preserved in the General Microorganism Center of China Microbiological Culture Collection and Management Committee at April third, 2014, and the culture preservation number is CGMCC NO.8993. The strain is cultured in an LB culture solution in a shaking way at the temperature of 28 DEG C and the speed of 180 rpm for 12-24 hours, a microbial agent finished product with a total viable bacteria concentration of 1*109-1*1010 CFU / mL is prepared, and after being diluted for 10-100 times, the microbial agent finished product is used for seed soaking treatment during pepper planting or root irrigating treatment during transplanting. The bacteriostasis effect is very remarkable. Tests verify that the pepper disease preventing rate of the biocontrol microbial agent can reach 69.06% under room temperature conditions, and the pepper yield increase range reaches 38.5%.

The invention relates to a biocontrol bacterial strain SM16 for preventing pear die-back, belonging to the technical field of plant protection. The strain is bacillus subtilis prepared by the following steps of: vaccinating the strain in culture liquid; fermenting at 30 DEG C and 250 rpm for 20-24 h; then, centrifuging at 6000 rpm for 10 min; and preparing a bacterial agent from wet bacteria and a cell preservation solution in a ratio of 1:40 (g / ml), wherein the total concentration of live viable bacteria in a finished product is 1*109-1*1012 cfu / ml. When the biocontrol bacterial strain SM16 for preventing pear die-back is used in a fruit garden and 25 percent and 75 percent of pear flowers are blossomed, the bacterial agent is diluted into 1*108 cfu / ml by adding water and sprayed on the flower surfaces and has the pear die-back prevention effect over 57-68 percent.

The invention belongs to the technical field of plant protection, disclosing a biocontrol bacterial strain YP1 for preventing and curing wheat takeall and an application thereof. The biocontrol bacterial strain YP1 is Pseudomonas fluorescens and is reserved in the China General Microbiological Culture Collection Center on June 1, 2011, and the bacterial strain collection number is CGMCC (China General Microbiological Culture Collection Center) No.4922. In the biocontrol bactericide prepared with the biocontrol bacterial strain YP1, the total concentration of the viable bacteria of the YP1 is 1*10<9>-1*10<10> CFU (colony-forming unit) / mL. The biocontrol bacterial strain can be used for preventing and curing the wheat takeall.

The invention discloses an application of a biocontrol strain BS102 to preventing crops from being polluted by aflatoxin. The biocontrol strain BS102 is bacillus subtilis BS102, with collection number of CGMCC No.7728. The application method comprises the steps of preparing a biocontrol agent of the biocontrol strain BS102 and applying the biocontrol agent to the crops. The biocontrol strain has strong antagonistic action on aspergillus flavus and can be used for preventing pollution caused by aflatoxin. The invention provides a biocontrol method for pollution caused by aflatoxin. As chemical pesticides are not used, the biocontrol strain has the advantages of no toxicity, low possibility of having resistance to drugs, environmental and biological safety and the like, and has extensive application prospects.

The invention discloses a biocontrol strain EWN23 for preventing and treating litchi downy blight and application thereof. The biocontrol strain EWN23 for preventing and treating the litchi downy blight is preserved at Guangdong Microbial Strain Preservation Center on November 15, 2016, and the preservation number of the strain is GDMCC No. 60111. The biocontrol strain EWN23 is used for preventingand treating the litchi downy blight and keeping the freshness of litchi. The bacillus amyloliquefaciens obtained by the invention is sourced from litchi endophytic bacteria and can be well planted on the surface of a litchi fruit; and when the biocontrol strain EWN23 is used to prevent and treat the disease of the litchi, the control of the disease is only reflected as the increment or reductionof numbers of a microorganism among specific populations, the type of the microorganism population on the litchi is not changed, the stability of the population is not influenced, in addition, sincethe biocontrol strain co-exists with the litchi for a long time and the biocontrol strain and the litchi can benefit each other, a series of existing mechanisms similar to the litchi can be formed, and when the biocontrol strain EWN23 is sprayed onto the surface of the litchi to prevent and treat the diseases, more safety and reliability in eating the litchi fruits and litchi processed products can be realized.

The invention belongs to the technical field of plant protection, which discloses a biocontrol strain PA-2 (Pseudomonas Aeruginosa) for preventing and curing the tobacco angular leaf spot. The strain is collected in the China General Microbiological Culture Collection Center on 27th March, 2012 with a collection number of CGMCC (China General Microbiological Culture Collection Center) No.5937. The total concentration of biocontrol bacterial liquid prepared from the biocontrol strain PA-2 is 1*10<9>-1*10<10>CFU (Colony Forming Units) / mL. The biocontrol bacterial liquid can be used for preventing and curing the tobacco angular leaf spot and the biocontrol strain is a strain with a great research and development value.

The invention belongs to the technical field of plant protection, and relates to a biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and an application thereof. The bacterial strain BS1 is bacillus cereus and is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC NO.4736. The biological prevention bacterial strain BS1 for preventing and controlling greenhouse cucumber downy mildew takes the bacillus cereus of which the collection number is CGMCC NO.4736 as an effective ingredient, wherein the viable bacteria concentration of the BS1 is 1*10<9>-10<10>CFU (colony-forming unit) / ml. After being diluted by 100 times, the biological prevention bacterial strain is sprayed after the cucumber is transplanted for 20d, and the investigation shows that the biological prevention effect on the cucumber downy mildew is 60.64% after 8d; and the biological prevention effect is 65.43% when spraying and root irrigation are simultaneously carried out. The bacillus cereus BS1 can be used for preparing the pesticide capable of preventing and controlling the greenhouse cucumber downy mildew.

A novel bridging ligand and its metallic match for the catalytic polymerization of olefin are disclosed. Said metallic match as the precursor of catalyst can be used in conjunction with cocatalyst for higher catalytic activity. The resultant resin has wide or dual-peak distribution of molecular weights.

The invention discloses a strain 1LN2 for preventing and treating a rice sheath blight disease. The classification and nomenclature of the strain 1LN2 are pseudomonas sp., and the strain 1LN2 is preserved with the preservation number CGMCC No.11361 in China General Microbiological Culture Collection Center on September 10, 2015. The invention further discloses a biocontrol agent prepared from the strain 1LN2 for preventing and treating the rice sheath blight disease. The invention further discloses application of the biocontrol agent to prevention and treatment of the rice sheath blight disease. The biocontrol agent used in a greenhouse is capable of preventing and treating the rice sheath blight disease to a certain extent, greenhouse control effect is up to 60.25%, and field control effect after field experiment treatment is performed for one month is up to 50.31%; a 1LN2 strain preparation is capable of increasing protein content of rice, decreasing amylose content, improving gel consistency and increasing rice yield to a certain degree, and the yield increase rate obtained through the 1LN2 strain preparation reaches 7.1% compared with that obtained through a conventional chemical agent.

The invention discloses a biocontrol strain for preventing and controlling fusarium wilt of watermelon and application thereof. The biocontrol strain is classified and named as Bacillus subtilis XGY13-1, is preserved in China General Microbiological Culture Collection Center and has the preservation number of CGMCC NO. 8973. The single colony of the biocontrol strain for preventing and controlling fusarium wilt of watermelon is picked into an LB liquid culture medium and is subjected to shaking culture on a 180rpm shaker at 30 DEG C for 10 hours to obtain a seed liquid, and the liquid seed is inoculated onto a fermentation culture medium at an inoculation amount of 1% (v / v) and subjected to shaking culture on a 180rpm shaker for 8-9 days at 30 DEG C to prepare a biocontrol microbial agent. The application method comprises the following steps: diluting the biocontrol microbial agent by 20-100 times, soaking watermelon seeds when the watermelon is planted or carrying out irrigating root treatment when watermelon seedlings are transplanted, wherein the fusarium wilt of watermelon is prevented and controlled by 62.36%. Meanwhile, the microbial agent has a growth-promoting function.

The invention discloses a biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases and an application of the biocontrol strain PNC25. The biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases refers to Exiguobacterium acetylicum, collected in the Guangdong Microbial Culture Collection Center, with the collection number of GDMCC No. 60108, onthe date of November 15th, 2016. The Exiguobacterium acetylicum can suppress the litchi downy blight diseases, and has excellent biocontrol effect on the litchi downy blight diseases on multiple experiment conditions. Besides the application in preventing and controlling the litchi downy blight diseases, the Exiguobacterium acetylicum has the good effect in keeping litchi fresh, and accordingly can be used for preparation of litchi preservatives.

The invention provides a PS mixture of compound biocontrol fungicide for preventing the soil-borne diseases of vegetables, belonging to the technical field of crop disease prevention and cure. The PS mixture consists of two biocontrol bacteria, namely PX35 and SM21 respectively. The PS mixture is prepared through the following steps: culturing the two bacteria by shaking at 180 rpm at the temperature of 37 DEG C in a PDA (potato dextrose agar) culture solution for 20 to 24 hours respectively; then, centrifugating at 4,000g for 10min; and preparing the PS mixture from humidin and thallus preservative fluid by a ratio of 1:40 and further evenly mixing by a ratio of 1:1, wherein, the total concentration of viable bacteria in the finished product is 1*10 to 1*10 cfu / ml. Under the greenhouse condition, the control effect of the PS mixture on the bacterial wilt of solanaceae crops is 67.40%, the control effect thereof on the plague of solanaceae crops is over 77%, the control effect thereof on vegetable root knot nematode is 58.59%, and the effect of growth promotion is obvious; under the field condition, the control effect is stable, the yield can be increased by 50% to 80%; and at the same time, the PS mixture can prevent the soil from hardening.