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Bacterial strain XY21 preventing and curing glasshouse vegetable bacterial wilt

A XY21, bacterial wilt technology, applied in bacteria, microorganism-based methods, biocides, etc., can solve the problems of poor screening purpose and complex group formation, and achieve the effect of improving control effect, increasing income and saving expenses

Inactive Publication Date: 2008-03-12
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, because the composition groups of R. solanacearum strains are very complex, and other microorganisms used for biological control of R. solanacearum are generally screened from various soils, and typical or single strains of R. solanacearum are used as indicator bacteria, the purpose of screening is not strong. Antagonistic microorganisms also have certain limitations, resulting in no biocontrol agents that can successfully control different R. solanacearum bacteria in practical applications.

Method used

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  • Bacterial strain XY21 preventing and curing glasshouse vegetable bacterial wilt

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Embodiment Construction

[0018] 1 Identification of strains

[0019] A biocontrol bacterial strain XY21 for preventing and controlling bacterial wilt of greenhouse vegetables (Phytophthora capsici) is specially used for preventing and controlling bacterial wilt of greenhouse vegetables such as peppers, tomatoes, and eggplants. The contriver has carried out research from the plate antagonistic activity of XY21 bacterial strain, produces cellulase, protease, chitinase activity and produces siderophilic element assay, and has carried out greenhouse and field control effect research to it, finds that XY21 bacterial strain preparation has very good effect. Good disease prevention and production increase effects have great potential to be developed into commercial live bacteria biocontrol preparations.

[0020] The XY21 strain is Serratia sp.; isolated from Zhenjiang forest soil, XY21 produces a single colony on LB medium for 10 hours, and after 24 hours of culture, the colony is milky white, uniform in tex...

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Abstract

The invention provides a biologic bacterial strain XY21 for preventing and treating bacterial wilt in greenhouse vegetables, and pertains to the technical field for preventing and treating diseases in crops. The XY21 is verified to be Serratia sp., the culture preservation no. is CGMCC NO.2059. The XY21 bacterial strain expresses good colonization surrounding the roots of tomatoes. After soaking the culture for 14 days, the colonized volume of the bacterial strain surrounding a root of tomato tree is 5.05 is multiplied by 10<7>cfu / g soil. If the wet bacterial strain is mixed with a lab thallus-preservation solution in a proportion of 1:40 (g / ml), the bacterial strain can be stored 2-3 years under room temperature. Tests show that, by application XY21 bacterial strain in fields, the prevention effect is 64.84% and 79.80% respectively, the outputs of the diseased fields increased respectively 31.8% and 45.8%. The invention is of high prospective to be developed into commercial live-vaccine biologic preventing preparations.

Description

1. Technical field [0001] The invention relates to a single bacterial strain for preventing and curing greenhouse vegetable bacterial wilt, belongs to the technical field of crop disease prevention and treatment, and is specially used for the pollution-free prevention and treatment of vegetable bacterial wilt. 2. Technical background [0002] Bacterial wilt is an important plant soil-borne disease, which commonly occurs in tropical, subtropical and temperate regions and causes serious damage. Potatoes, tomatoes, and tobacco, the main crops in my country, are all damaged by this fungus, causing losses to varying degrees. At present, the planting area of ​​vegetables in protected areas in my country is constantly expanding. Because the temperature and humidity conditions in protected areas are suitable for the growth of R. solanacearum, and Ralstonia solanacearum has a wide range of hosts, it can infect most of the Solanaceae vegetables. Therefore, it is easy to cause continuo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02C12R1/425
Inventor 郭坚华薛庆云刘红霞
Owner NANJING AGRICULTURAL UNIVERSITY
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