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Bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof

A biocontrol strain, 1JN2 technology, applied in the field of agricultural biology, can solve the problems of pesticide residues, low control effect, pathogenic bacteria resistance, etc., and achieve the effect of saving expenses and benefiting export trade

Active Publication Date: 2012-07-25
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacterial fruit spot of watermelon is a devastating bacterial disease. It is not easy to be found during the field growth period, and the onset occurs suddenly and develops rapidly. Since no better method for seed treatment has been found, the chemical agents currently used in production There are agricultural streptomycin, which can kill the disease, phytomycin, bactericide, Ye Shuangqing, Garenon, copper succinate, etc. Although the chemical agents have a certain control effect on the disease, the control effect is not high, and Extensive use of chemical fungicides often causes environmental pollution, pesticide residues, and pathogenic bacteria resistance

Method used

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  • Bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof
  • Bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof
  • Bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A biocontrol strain 1JN2 for preventing and controlling bacterial fruit spot disease of watermelon is Bacillus axarquiensis, which is isolated from ginger stems in Laiwu City, Shandong Province. The specific separation, screening and identification methods are as follows:

[0018] 1 Isolation of biocontrol strain 1JN2

[0019] Cut off 3 g of ginger stems, soak in 1 mL of 1% sodium hypochlorite for 5 minutes, soak in 70% alcohol for 2 minutes, and wash with sterile water for 3 times. (Take 0.1 mL of the last cleaning solution and apply R 2 A, or add 0.1mL of the last cleaning solution to R 2 A culture medium, shake cultured at 30°C, and if it grows sterile after 48h, the surface is considered to be clean). Grind the surface-sterilized stems with a sterilized pestle, add 3 mL of sterile water, filter with sterile cotton cloth, dilute 10 times with sterile water, take 0.1 mL of LB plate, repeat 3 times for each point, and incubate at 28°C 48h, count, pick all colonies ...

Embodiment 2

[0031] Example 2 Preparation of Bacillus cereus 1JN2 biocontrol agent

[0032] The above-mentioned biocontrol strain 1JN2 (CGMCC NO.5731) for the prevention and treatment of watermelon bacterial fruit spot is shaken and cultured in LB medium at 28°C and 180rpm for 12-16h, then centrifuged at 6000rpm for 10min, diluted with sterilized water to prepare a bacterial agent. The total concentration of viable bacteria in the finished product is 1×10 9 -1×10 10 CFU / mL, the biocontrol agent is hereinafter referred to as Bacillus cereus 1JN2 biocontrol agent.

Embodiment 3

[0033] Example 3 Greenhouse growth promotion test of Bacillus cereus 1JN2

[0034]The germinated watermelon seeds were sown in pots with sterile soil, one seed per pot, 16 / 8h photoperiod. When the watermelon grows to the 6-leaf stage, the suspended Bacillus cereus 1JN2 biocontrol agent (prepared in Example 2, the same below) is treated with 10 8 The concentration of CFU / ml was sprayed on the surface of the leaves in the form of a mist with a manual sprayer, so that the leaves were wet and the inoculant did not flow down. Cultured in a greenhouse at 28°C and above 70% relative humidity. After 30 days of inoculation with biocontrol bacteria, the plants of the treatment group and the control group were taken respectively, and the soil at the roots was carefully removed. After cleaning, they were naturally air-dried. , the fresh weight of the plant. Dry weight of plants was measured after drying at 60 °C for 72 h.

[0035] Increase in biomass (%)=(dry weight of plants in treatm...

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Abstract

The invention belongs to the technical field of crop disease prevention and treatment and discloses a bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof. The bio-control bacteria strain 1JN2 is named as Bacillus axarquiensis in classification, and is filed at General Microbiological Center of China General Microbiological Culture Collection Committee with CGMCC No.5731 on January 16, 2012. The bio-control bacteria strain 1JN2 is cultured in LB (luria-bertani) broth at the temperature of 28 DEG C by vibration of 180rpm for 12-16 hours, centrifuged by 6000rpm for 10 minutes, diluted by sterilization water to prepare bactericide, and total concentration of viable bacteria in the finished bactericide is 1X10<9>-1X10<10>CFU / ml. The experiment results in a greenhouse indicates that the bio-control bactericide with the 1JN2 has evident growth acceleration effect to watermelons, biomass live weight is increased to 55.92% and evident prevention effect to disease of fruit blotches of the watermelons, disease incidence can be reduced by 50.13%.

Description

technical field [0001] The invention belongs to the technical field of agricultural biotechnology, and relates to a biocontrol strain 1JN2 for preventing and treating bacterial fruit spot of watermelon and its application. Background technique [0002] Bacterial fruit blotch of watermelon (BFB) is a dangerous quarantine disease on watermelon, also known as bacterial blotch, which was first discovered by Crall and Schenck in Florida in 1969. The symptoms of fruit spot disease are only described, but the identification of pathogenic bacteria is not done; so far, fruit spot disease has been reported in many provinces and cities in my country, such as Xinjiang, Shaanxi, Hebei, Shanxi, Jilin, Fujian, Gansu and other places. The disease mainly damages the fruit, causing watermelon fruit to rot, etc., which can cause devastating losses. Once the disease occurs, drug control is basically ineffective. And the infected seeds will become an important source of primary infection in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/07
Inventor 郭坚华蒋春号武芳
Owner NANJING AGRICULTURAL UNIVERSITY
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