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Biocontrol strain EWN23 for preventing and treating litchi downy blight and application thereof

A biocontrol strain, Phytophthora lychee technology, applied in the direction of application, bacteria, biocide, etc., can solve the problems of pathogen resistance, environmental pollution, etc., achieve cost saving, easy cultivation conditions, excellent biocontrol and preservation effect of ability

Inactive Publication Date: 2019-03-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effective measures for the control of litchi frost blight are mainly chemical control. However, due to the large-scale use of chemical pesticides that may induce environmental pollution and the problem of pathogenic drug resistance has become increasingly prominent, research and development of low-toxic, non-residue biological The use of source pesticides in the prevention and control of litchi diseases is of great significance for controlling the amount of pesticide residues, ensuring food safety and improving the international competitiveness of products

Method used

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  • Biocontrol strain EWN23 for preventing and treating litchi downy blight and application thereof
  • Biocontrol strain EWN23 for preventing and treating litchi downy blight and application thereof
  • Biocontrol strain EWN23 for preventing and treating litchi downy blight and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Source of the strain: EWN23 was isolated from the pericarp of "Yuan Nuo" in a litchi orchard in Huilai, Jieyang City, Guangdong Province.

[0029] Samples were collected in a litchi forest in Guangdong Province in July 2015. The five-point sampling method is adopted, and the interval between each point is at least 10m. The rhizosphere soil of three healthy plants was collected at each point. The collected samples were quickly put into numbered plastic bags and brought back to the laboratory for separation. The method is as follows: cut 3 g of the pericarp and add it to 27 mL of sterilized water (including sterilized glass beads), oscillate on a shaker at 180 rpm for 0.5 h, and let it stand for 5 min to obtain a 10-1 dilution. 10 -4 、10 -5 、10 -6 Three gradient 0.1mL plates were coated with LB, cultured at 28°C for 48h, and plates with 50-300 colonies were selected, counted and all colonies were picked, purified, and stored at -70°C in 40% glycerol for later use (Be...

Embodiment 2

[0040] Embodiment 2 bacterial agent preparation

[0041] (1) Cultivation of seed bacteria liquid: Inoculate EWN23 (GDMCC No.60111) into LB culture liquid, culture at 28°C and 200 rpm for 24 hours, and after 16 hours, take samples every 3 hours in the ultra-clean workbench and measure the OD value at 600nm During the measurement process, use the uninoculated culture solution to adjust to zero, until the OD value of the seed bacteria solution is between 0.5 and 0.8, the culture is ended, and the seed bacteria solution is obtained;

[0042] (2) Preparation of wet bacteria: ferment and culture the seed bacteria solution at a volume ratio of 1:500, shake and culture at 28°C and 200rpm for 24 hours to obtain a biocontrol agent with a concentration of viable bacteria of 1×10 8 ~10 9 CFU / mL.

Embodiment 3

[0043] Example 3 Evaluation of the indoor (LT, laboratory trial) disease prevention effect of biocontrol bacteria EWN23 on litchi fruit / leaf

[0044] The healthy fruits / leaves picked in the field are processed as follows: A. Dilute the stock solution of biocontrol bacteria to a final concentration of 5*10 7 CFU / mL spray fruit / dilute to final concentration 1*10 7 CFU / mL spray leaves; B. LB solution of the same dilution multiple. Set up 3 repetitions for each treatment, each repetition has 30 fruits / 5 branches, and the fruits / branches are placed in a fresh-keeping box (the bottom of the box is covered with sterilized filter paper, 18 cm in diameter, moistened with sterilized water, and the size of the fresh-keeping box is 323*220 *100mm, the manufacturer is Hualong Plastic Factory, Chancheng District, Foshan City). The bacterial solution and the control group were sprayed with 300mL for each treatment (3 repetitions). 24hpt (hours post treatment) spray inoculation of pathogen...

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Abstract

The invention discloses a biocontrol strain EWN23 for preventing and treating litchi downy blight and application thereof. The biocontrol strain EWN23 for preventing and treating the litchi downy blight is preserved at Guangdong Microbial Strain Preservation Center on November 15, 2016, and the preservation number of the strain is GDMCC No. 60111. The biocontrol strain EWN23 is used for preventingand treating the litchi downy blight and keeping the freshness of litchi. The bacillus amyloliquefaciens obtained by the invention is sourced from litchi endophytic bacteria and can be well planted on the surface of a litchi fruit; and when the biocontrol strain EWN23 is used to prevent and treat the disease of the litchi, the control of the disease is only reflected as the increment or reductionof numbers of a microorganism among specific populations, the type of the microorganism population on the litchi is not changed, the stability of the population is not influenced, in addition, sincethe biocontrol strain co-exists with the litchi for a long time and the biocontrol strain and the litchi can benefit each other, a series of existing mechanisms similar to the litchi can be formed, and when the biocontrol strain EWN23 is sprayed onto the surface of the litchi to prevent and treat the diseases, more safety and reliability in eating the litchi fruits and litchi processed products can be realized.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a biocontrol strain EWN23 for preventing and treating litchi frost blight and its application. Background technique [0002] Litchi (Litchi chinensis Sonn) is an important subtropical economic crop in the southern region. It is known as the "Chinese Precious Fruit" and has a high economic value. It is one of the most competitive fruits in the international market in my country. In normal temperature or low temperature storage after harvesting litchi, the fruit is easy to brown and rot, and the main diseases that cause fruit rot and deterioration include litchi anthracnose, sour rot, frost blight, etc. Among them, Peronophythora litchii Chen ex Ko et al .) caused the most severe frost blight damage (Kao et al., 1980; Qi Peikun et al., 1984). The fungus likes high humidity and can infect young leaves, flower spikes, young fruits, branches, and fruit stalks, resulting in flo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 姜子德郑丽习平根孔广辉
Owner SOUTH CHINA AGRI UNIV
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